中国媒介生物学及控制杂志

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基因工程灭蚊幼蓝藻的现场初步观察

阎歌1; 刘相萍1; 张晓静1; 江洪涛1; 甄天民1; 王怀位1; 孔任秋1; 徐旭东1; 郝延玉2; 程义亮1(指导)   

  1. 1.山东省寄生虫病防治研究所(济宁,272133);2.山东省平阴县卫生防疫站
  • 出版日期:1996-04-20 发布日期:1996-04-20

Preliminary Field Observation on Genetically Engineered Larvicidal Cyanobacterium

Yan Ge;Liu Xiang-ping;Zhang Xiao-jing;et al   

  1. Shandong Institute of Parasitic Diseases,Jinning Shandong 272133
  • Online:1996-04-20 Published:1996-04-20

摘要: 将转入球形芽孢杆菌毒蛋白基因表达质粒的工程藻Anabaena subtropic(pDc26)大量培养后,应用于山东省平阴县进行现场观察。结果表明:工程藻在9.41×105cells/ml浓度以上时,48h可杀死99~100%蚊幼虫;在9.13×104cells/ml浓度时,4d可杀死99%以上蚊幼虫,连续观察2个多月,工程藻仍有明显杀蚊效果,实验组最多14条/勺幼虫,而对照组210条/勺左右。观察还发现,工程藻对库蚊幼虫杀灭效果最好,其次为伊蚊,但高浓度时,对伊蚊仍有明显杀灭效果;由于按蚊幼虫密度太低,未能观察。此结果比单独使用球形芽孢杆菌杀灭蚊幼虫有明显提高,持续时间也延长,这将为蚊虫的持续控制提供一个新的有力武器。

关键词: 基因工程藻, 球形芽孢杆菌, 现场, 效果

Abstract: After Anabaena subtropic (pDC26) of engineered larvicidal cyanobacterium was cultured in the large,its larvicidal effect was observed in the fileds of Pingyin county of Shandong Province,The results showed that A.subtropic(pDC26) had high toxicity against the larvae of Culex pipiens and Aedes albopictus,it killed 99 100% larvae in 48hrs at the concentration of 9.41×105,and 8.95×106,and above 99% in 72h at 9.13×104. It had obviously toxicity against the mosquito larvae continuously for two months,The effect of toxicity against the larvae of Culex pipiens was better than that of Aedes albopictus.Therefore the genetically engineered cyanobacteria opened up new possibilities for mosquito control.