关键词: 家蝇, 高效氯氰菊酯, 抗药性, 抗性选育

Abstract:

Objective　To determine the mechanisms of development and hereditary patterns of the resistance against pyrethroids in Musca domestica using beta?cypermethrin-resistant strain domestically produced by selective breeding, providing scientific  basis  for  the establishment of practical management strategies to restrain the development of insecticide resistance for effective control of houseflies.  Methods　Beta-cypermethrin of median lethal  concentration  applied  to  each  generation  of  susceptible  M. domestica  strains, the resistance selective breeding was performed in compliance with the National Standard  GB 13917.1-13917.8－92, Laboratory efficacy test methods and criterions of public health insecticides for pesticide registration. Bioassay methods were utilized to determine the LD50 of generations of M. domestica from which the resistance index was generated. Results　Continuous affection of beta?cypermethrin on twenty?two generations of selective breeding gave arise to rapid development of strong resistance in susceptible M. domestica strains, resulting in a resistant line with the resistance index of 104.23 folds. Conclusion　The occurrence and development of resistance in M. domestica was the result of the directional selection favoring traits of resistance genes exerted by the insecticide. The stronger the selection pressure is, the faster the resistance develops. Different methods of insecticide application pose significant influence on selective breeding for resistance.

Key words: Musca domestica, Beta-cypermethrin, Insecticide resistance, Selective breeding for resistance