中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (2): 119-121.

• 论著 • 上一篇    下一篇

滞育对家蝇幼虫蛋白质和核酸的影响

刘流贺莉芳刘晖晏容万启惠    

  1. 遵义医学院寄生虫学教研室(贵州 遵义563003)
  • 收稿日期:2008-10-09 出版日期:2009-04-20 发布日期:2009-04-20
  • 通讯作者: 万启惠,硕士生导师, Email: qhwan@sina.com
  • 作者简介:刘流(1976-),女,贵州六盘水人,硕士,主要从事寄生虫感染与免疫研究。
  • 基金资助:

    遵义医学院硕士启动基金[院发(2007)004]

The impact of diapause on the protein and nucleic acid of Musca domestica larva

 LIU Liu, HE Li-Fang, LIU Hui, YAN Rong, MO Qi-Hui   

  1. Zunyi Medical College, Zunyi, Guizhou 563003, China
  • Received:2008-10-09 Online:2009-04-20 Published:2009-04-20
  • Contact: WAN Qi-hui, Email: qhwan@sina.com

摘要:


  【摘要】 目的 观察家蝇3龄幼虫滞育前后血淋巴中蛋白质及组织匀浆脱氧核糖核酸(DNA)、核糖核酸(RNA)的变化,探讨家蝇幼虫滞育发生的生化机制。方法 低温诱导家蝇3龄幼虫滞育,经SDS?聚丙烯酰胺凝胶电泳观察非滞育组及各滞育组血淋巴蛋白组分变化;紫外分光光度计测定非滞育组及各滞育组RNA和DNA含量。结果 (1)滞育组和非滞育组家蝇幼虫蛋白条带数目无变化,只是滞育20 d组的蛋白含量明显高于非滞育组,其中以85 kd处最明显,光密度值为203 475.69。(2)各滞育组DNA均比非滞育组明显降低;滞育10 d组、15 d组RNA明显降低,滞育20 d 组则明显升高;RNA/DNA在滞育10 d组、15 d组明显降低分别为0.598、0.546 μg/mg,滞育20 d组则明显升高为1.337 μg/mg。结论 滞育家蝇幼虫体内可能无滞育关联蛋白(DAP)的存在,RNA/DNA比值的降低和升高可作为家蝇幼虫滞育开始及滞育结束的信号。

关键词: 家蝇幼虫, 滞育, 蛋白质, 核酸

Abstract:

  【Abstract】 Objective To observe the change of protein in the hemlymph and DNA and RNA in the tissue from the 3rd stage larva of Musca domestica  before and after diapause. To explore the biochemistry mechanism of M.domestica larvae diapause. Methods The diapause of  the 3rd stage larva of M.domestica  was inducted by low temperature.(1)The change of hemlymph protein in the diapause and the nondiapause groups were observed by polyacrylamide gel electrophoresis?SDS. (2) The RNA and DNA in the tissue of the diapause and the nondiapause groups were extracted and the contents were determined by ultraviolet spectrophotometer. Results (1) The numbers of protein straps in the diapause groups  were similar to that in nondiapause group.But the content of the protein in the groups after diapause for 20 d was obviously higher than that in the  nondiapause group. The optical density value was 203 475.69 at 85 kd.(2)The DNA content in all diapause groups was much lower than that of nondiapause group. The RNA content obviously decreased after diapause for 10 d and 15 d, but it obviously increased after diapause for 20 d. The contents of RNA and DNA after diapause for 10 d and 15 d decreased to 0.598, 0.546 μg/mg, but they increased to 1.337 μg/mg  after  diapause for 20 d.   Conclusion  Perhaps  there  was no diapause associated protein in diapause M.domestica larva. The decrease and increase of the proportion of RNA to DNA could be as the signal of beginning and ending of M.domestica larva diapause.

Key words: Musca domestica larva, Diapause, Protein, Nucleic acid

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