中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (3): 246-248.

• 论著 • 上一篇    下一篇

长春龙嘉国际机场口岸汉坦病毒基因型研究

贺晨1,邵丽筠2,黄新功2,于雅琴1,刘娅1   

  1. 1 吉林大学公共卫生学院(长春 130021); 2 吉林出入境检验检疫局
  • 收稿日期:2009-02-02 出版日期:2009-06-20 发布日期:2009-06-20
  • 作者简介:贺晨(1981-),男, 甘肃临洮人,博士研究生,从事卫生检疫工作。

Study on genotype of Hantavirus at Changchun Longjia international airport

 HE Chen, SHAO Li-Jun, HUANG Xin-Gong, XU Ya-Qin, LIU Ya   

  1. Jilin Entry?Exit Inspection and Quarantine  Bureau, Changchun, Jilin 130021, China
  • Received:2009-02-02 Online:2009-06-20 Published:2009-06-20

摘要:

  【摘要】 目的 研究长春龙嘉国际机场口岸地区鼠间汉坦病毒(HV)基因型差异。方法 2006年4月至2007年3月,采用夹日法捕捉啮齿类动物,用免疫荧光法检测阳性标本,应用反转录-聚合酶链反应(RT-PCR)扩增技术对特异性核苷酸序列进行扩增并进行测序分析。结果 共捕获鼠类195只,10只携带HV。其中4只为汉滩型病毒(HTNV),6只为汉城型病毒(SEOV)。基因测序分析发现,此次研究中的HV发生了一定变异,但并未发现新的亚型。结论 长春龙嘉国际机场口岸宿主动物所携带的HV基因型包括HTN型和SEO型,对该地区肾综合征出血热的防控有重要指导意义。

关键词: 口岸, 汉坦病毒, 基因

Abstract:

  【Abstract】 Objective To study the difference of Hantavirus genotype among rats at Changchun Longjia international airport. Methods From Apr. 2006 to Mar. 2007, rodents were captured by snap trap (mouse clamp). Samples were detected by direct immunofluorescence technique. The partial M fragments were amplified with nested reverse transcription polymerase chain reaction (RT-PCR) using Hantavirus genotype-specific primers, and its sequence was analyzed. Results There were 195 mice captured, and about 10 mice took the Hantavirus. There were 4 Hantaan viruses and 6 Seoul viruses. Nucleotide sequence homology analysis showed that there was some mutation in the Hantavirus and no found new sub-genotype. Conclusion There are Hantaan viruses and Seoul viruses at Changchun Longjia international airport, which is valuable for the prevention and control of hemorrhagic fever with renal syndrome.

Key words: Port, Hantavirus, Gene

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