猪种布鲁氏菌生物1型野毒株和2号菌苗的鉴别诊断

中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (3): 237-239.

猪种布鲁氏菌生物1型野毒株和2号菌苗的鉴别诊断

姜海，崔步云，赵鸿雁，朴东日，李兰玉

中国疾病预防控制中心传染病预防控制所人兽共患病室（北京 102206）

收稿日期:2009-03-26 出版日期:2009-06-20 发布日期:2009-06-20
通讯作者: 崔步云，Email: cuibuyun@icdc.cn
作者简介:姜海（1974-），男，博士，主要从事布病分子生物学研究。
基金资助:
国家高技术研究发展计划（“863”项目）（2007AA02Z410）

Differential diagnosis of Brucella suis biovar 1 wild strain and S2 vaccine strain

JIANG Hai, CUI Bu-Yun, ZHAO Hong-Yan, PIAO Dong-Ri, LI Lan-Yu

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received:2009-03-26 Online:2009-06-20 Published:2009-06-20
Contact: CUI Bu-yun, Email: cuibuyun@icdc.cn

摘要/Abstract

摘要：

　【摘要】　目的　寻找猪种布鲁氏菌生物1型野毒株（1330S）和猪种布鲁氏菌2号菌株（S2）的鉴别诊断方法。方法　采用牛、羊、绵羊、猪种型聚合酶链反应（AMOS?PCR）、脉冲场凝胶电泳（PFGE）和多位点可变数量串联重复序列（MLVA）3种方法对21株1330S菌株进行鉴别。结果　AMOS?PCR和PFGE方法未能区分两类菌株；MLVA方法发现1330S和S2菌株在Bru9和Bru16两位点存在差异。结论　MLVA方法可以作为两类菌株鉴别诊断的方法之一。

关键词: 猪种布鲁氏菌生物1型, 菌苗株, 鉴别诊断

Abstract:

　　【Abstract】　Objective　To study the method to identify Brucella suis biovar 1 wild strain and S2 vaccine strain. Methods A total of 21 strains of B.suis biovar 1 wild strain were analyzed by AMOS-PCR, PFGE and multiple-locus variable-number tandem-repeat analysis (MLVA). Results　B.suis biovar 1 and S2 could be discriminated by MLVA Bru9 and Bru16. Conclusion　The MLVA assay can be applied to identification of wild strains and vaccine strain and can be proposed to as a complement of classical biotyping methods.

Key words: Brucella suis biovar 1, Vaccine strain, Differential diagnosis

中图分类号:

R516.7

姜海，崔步云，赵鸿雁，朴东日，李兰玉. 猪种布鲁氏菌生物1型野毒株和2号菌苗的鉴别诊断[J]. 中国媒介生物学及控制杂志, 2009, 20(3): 237-239.

JIANG Hai, CUI Bu-Yun, ZHAO Hong-Yan, PIAO Dong-Ri, LI Lan-Yu. Differential diagnosis of Brucella suis biovar 1 wild strain and S2 vaccine strain[J]. Chines Journal of Vector Biology and Control, 2009, 20(3): 237-239.