中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (4): 335-338.

• 论著 • 上一篇    下一篇

广藿香精油对白纹伊蚊的生物活性研究

曾冬琴1,彭映辉1,陈飞飞1,张云1,秦巧慧1,黄谊2   

  1. 1 中南林业科技大学生命科学与技术学院(长沙 410004);2 湖南省疾病预防控制中心
  • 收稿日期:2009-04-06 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 彭映辉,Email: pengyh999@163.com
  • 作者简介:曾冬琴(1984-),女,湖北人,硕士研究生,主要从事蚊虫防制及植物源农药研究工作。
  • 基金资助:

    湖南省自然科学基金(08JJ3028); 湖南省教育厅高等学校科学研究重点项目(06A083); 中南林业科技大学研究生科技创新基金(2007sx07); 湖南省研究生科技创新基金

Bioassay of Pogostemon cablin (Blanoa) Benth oil against Aedes albopictus Skuse

 ZENG Dong-Qin, PENG Ying-Hui, CHEN Fei-Fei, ZHANG Yun, QIN Qiao-Hui, HUANG Yi   

  1.  School of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
  • Received:2009-04-06 Online:2009-08-20 Published:2009-08-20
  • Contact: PENG Ying-hui, Email: pengyh999@163.com

摘要:

  【摘要】 目的 测定广藿香精油对白纹伊蚊的生物活性,筛选出一种对蚊虫具有驱杀活性的植物精油。方法 在实验室条件下,用浸液法测定水蒸汽蒸馏提取的广藿香精油对白纹伊蚊各龄期幼虫及蛹的毒杀活性;用改良三角瓶法测定其对白纹伊蚊成蚊的熏杀活性;采用个体涂肤有效保护时间测定法测试其对白纹伊蚊的驱避活性。结果 (1)广藿香精油对白纹伊蚊各龄期幼虫均有较强的毒杀活性,对1~4龄期幼虫及蛹24 h的LC50值分别是20、34、59、60和187 μg/ml;(2)广藿香精油对白纹伊蚊成蚊的熏杀快速有效,在18、36、54 μg/cm3三种熏蒸剂量下,对白纹伊蚊熏杀的KT50值分别是14.692、16.528和14.807 min;(3)以1.5、2.0和2.5 mg/cm2剂量的广藿香精油涂抹人体皮肤,其完全保护时间分别是(2.70±0.12) h、(4.40±0.10) h和(6.00±0.44) h。结论  广藿香精油对白纹伊蚊幼虫及成蚊均有较好的生物活性,具有研发成环保型蚊虫驱杀剂的潜力。

关键词: 广藿香, 精油, 白纹伊蚊, 生物活性

Abstract:

  【Abstract】 Objective To test the bioactivity of essential oil extracted from Pogostemon cablin (Blanoa) Benth against Aedes albopictus Skuse, so as to find a new kind of mosquito repellent. Methods Essential oil was extracted from P.cablin by steam distillation. The bioassay against larvae and pupae of Ae. albopictus was carried out by immersion method. The adulticidal of P.cablin against Ae.albopictus was investigated by modified flask method. The repellent activity of pure P.cablin oil to Ae. albopictus was  assayed  by  topical  bioassay method  on  the  skin.  Results (1) P.cablin  oil  had  strong  toxicity  to  larvae  and  pupae  of Ae.albopictus. The LC50  values  of  P.cablin  oil  to Ⅰ, Ⅱ, Ⅲ, Ⅳ instar  larvae  and  pupae of  Ae.albopictus were 20, 34, 59, 60 and 187 μg/ml after treatment for 24 h, respectively. (2) The fumigation efficacy of P.cablin oil to adult Ae.albopictus was quick and efficient, and the KT50 values against Ae.albopictus were 14.692, 16.528 and 14.807 min at the dosages of 18, 36 and 54 μg/cm3, respectively. (3) The 100% protection time offered by P.cablin oil on the skin were (2.70±0.12) h, (4.40±0.10) h and (6.00±0.44) h at dosages of 1.5, 2.0 and 2.5 mg/cm2, respectively. Conclusion P.cablin oil has good bioactivity to Ae.albopictus, which is a kind of potential environment?friendly mosquito?repellent.

Key words: Pogostemon cablin (Blanoa) Benth, Essential oil, Aedes albopictus, Bioassay

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