中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (4): 303-306.

• 论著 • 上一篇    下一篇

氯氟氰菊酯对德国小蠊保护酶活性的影响及新型防治策略研究

马义1,霍新北2,马民3,洪岸1   

  1. 1 暨南大学生物工程研究所(广州 510632); 2 山东省疾病预防控制中心病媒生物防制所; 3 暨南大学医学院
  • 收稿日期:2009-04-10 出版日期:2009-08-20 发布日期:2009-08-20
  • 通讯作者: 洪岸,Email: tha@jnu.edu.cn
  • 作者简介:马义(1973-),男,山东人,博士,主要从事细胞工程及其基因结构研究。
  • 基金资助:

    山东省卫生厅科技发展计划项目(HZ 0990)

Effect of lambda?cyhalothrin on protective enzyme in Blattella germanica and its new control measure

 MA Yi, HUO Xin-Bei, MA Min, HONG An   

  1. Bio-engineering Institute of Jinan University, Guangzhou, Guangdong 510632, China
  • Received:2009-04-10 Online:2009-08-20 Published:2009-08-20
  • Contact: HONG An, Email: tha@jnu.edu.cn

摘要:

  【摘要】 目的 通过研究高效氯氟氰菊酯对德国小蠊超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)2种保护酶活性的影响,进一步探讨高效氯氟氰菊酯的作用机制,为德国小蠊的防治工作提供理论基础和方法参考。方法 参照国标GB 13917.1-13917.8-92 《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氟氰菊酯,用酶活性测定试剂盒测定德国小蠊体内2种保护酶活性的时间变化。结果 不同浓度的高效氯氟氰菊酯对德国小蠊SOD和GPX的诱导作用存在时效上的不同,诱导强度与高效氯氟氰菊酯的浓度呈一定的正相关关系,144 h内SOD和GPX的酶活性均呈现“先升高—后降低—再升高” 的趋势。结论 低致死浓度高效氯氟氰菊酯对德国小蠊SOD和GPX有明显的诱导作用,SOD和GPX活性的升高与德国小蠊对高效氯氟氰菊酯等拟除虫菊酯类杀虫剂的抗药性及耐药性增强有关。

关键词: 德国小蠊, 高效氯氟氰菊酯, 超氧化物歧化酶, 谷胱甘肽过氧化物酶

Abstract:

  【Abstract】 Objective To further explore the action mechanism of lambda?cyhalothrin by researching the effect of it to Superoxide dismutase (SOD) and Glutathione peroxidase (GPX) of Blattella germanica, and to provide theoretical basis and useful methods for the control and prevention of B.germanica. Methods Lambda?cyhalothrin with different concentrations was sprayed on B.germanica according to the method of GB 13917.1-13917.8-92, and the activities of SOD and GPX were determined by kits.  Results The induction of lambda?cyhalothrin with different concentrations to SOD and GPX varied with time, and the induction effect had a positive relationship with the concentrations of lambda?cyhalothrin. The relative activities of SOD and GPX appeared the trend of “rise?decrease?rise” within 144 h. Conclusion The lower concentration of lambda?cyhalothrin has obvious induction to SOD and GPX, and the increase of SOD and GPX activity has the relationship to the resistance and tolerance of cockroach to lambda?cyhalothrin.

Key words: Blattella germanica, Lambda-cyhalothrin, Superoxide dismutase, Glutathione peroxidase

中图分类号: