多杀巴斯德菌感染的流行及致病特征研究进展

doi:10.11853/j.issn.1003.8280.2025.01.023

摘要/Abstract

摘要： 随着人类与家养和野生动物接触的日益频繁,人兽共患传染病的发生也愈发普遍。多杀巴斯德菌作为其中的一种重要病原体,其在动物群体中的高度流行性和对人类健康的潜在威胁不容忽视。该文回顾了多杀巴斯德菌的鉴定与分类方法,详细分析了其在动物和人类中的流行情况和宿主特异性,以及感染后的病理变化和免疫反应。该文为全面了解多杀巴斯德菌的基本特征及传播规律、优化防治多杀巴斯德菌感染提供参考。

关键词: 多杀巴斯德菌, 人兽共患病, 宿主特异性

Abstract: As human contact with domestic and wild animals increases, zoonotic infectious diseases are becoming increasingly common. Pasteurella multocida, as one of the important pathogens, cannot be ignored due to its high prevalence in animal populations and potential threat to human health. This paper reviews the identification and classification methods of P. multocida and analyzes in detail its prevalence and host specificity in animals and humans, as well as the pathological changes and immune responses after infection. This paper offers a reference for fully understanding the basic characteristics and transmission rules of P. multocida and optimizing the prevention and treatment of P. multocida infection.

Key words: Pasteurella multocida, Zoonosis, Host specificity

中图分类号:

R516.8

吕东月, 吴畏畏, 秦帅, 景怀琦, 王鑫. 多杀巴斯德菌感染的流行及致病特征研究进展[J]. 中国媒介生物学及控制杂志, 2025, 36(1): 137-143.

LYU Dong-yue, WU Wei-wei, QIN Shuai, JING Huai-qi, WANG Xin. Research progress on prevalence and pathogenic characteristics of Pasteurella multocida infection[J]. Chinese Journal of Vector Biology and Control, 2025, 36(1): 137-143.

参考文献

[1] Smith E, Miller E, Aguayo JM, et al. Genomic diversity and molecular epidemiology of Pasteurella multocida[J]. PloS One, 2021, 16（4）：e0249138. DOI：10.1371/journal.pone.0249138.
[2] Peng Z, Lin L, Wang X, et al. The public health concern of Pasteurella multocida should not be ignored[J]. Lancet Microbe, 2022, 3（8）：e560. DOI：10.1016/S2666-5247（22）00152-5.
[3] Haprper M, Boyce JD, Adler B. Pasteurella multocida pathogenesis：125 years after Pasteur[J]. FEMS Microbiol Lett, 2006, 265（1）：1-10. DOI：10.1111/j.1574-6968.2006.00442.x.
[4] Hunt Gerardo S, Citron DM, Claros MC, et al. Pasteurella multocida subsp. multocida and P. multocida subsp. septica differentiation by PCR fingerprinting and alpha-glucosidase activity[J]. J Clin Microbiol, 2001, 39（7）：2558-2564. DOI：10.1128/JCM.39.7.2558-2564.2001.
[5] Carter GR. Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types[J]. Am J Vet Res, 1955, 16（60）：481-484.
[6] Townsend KM, Boyce JD, Chung JY, et al. Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system[J]. J Clin Microbiol, 2001, 39（3）：924-929. DOI：10.1128/JCM.39.3.924-929.2001.
[7] Peng Z, Wang G, Wu GJ, et al. Isolation and identification of Pasteurella multocida from swine[J]. Chin Vet Sci, 2015, 45（9）：943-951. DOI：10.16656/j.issn.1673-4696.2015.09.011.（in Chinese）彭忠, 王庚, 吴广敬, 等.猪源多杀性巴氏杆菌的分离与鉴定[J]. 中国兽医科学, 2015, 45（9）：943-951. DOI：10.16656/j.issn.1673-4696.2015.09.011.
[8] Arumugam ND, Ajam N, Blackall PJ, et al. Capsular serotyping of Pasteurella multocida from various animal hosts：A comparison of phenotypic and genotypic methods[J]. Trop Biomed, 2011, 28（1）：55-63.
[9] Duan R, Lyu D, Qin S, et al. Pasteurella multocida strains of a novel capsular serotype and lethal to Marmota himalayana on Qinghai-Tibet plateau in China[J]. Int J Med Microbiol, 2024, 314：151597. DOI：10.1016/j.ijmm.2024.151597.
[10] Harper M, John M, Turni C, et al. Development of a rapid multiplex PCR assay to genotype Pasteurella multocida strains by use of the lipopolysaccharide outer core biosynthesis locus[J]. J Clin Microbiol, 2015, 53（2）：477-485. DOI：10.1128/JCM.02824-14.
[11] García-Alvarez A, Vela AI, San Martín E, et al. Characterization of Pasteurella multocida associated with ovine pneumonia using multi-locus sequence typing （MLST） and virulence-associated gene profile analysis and comparison with porcine isolates[J]. Vet Microbiol, 2017, 204： 180-187. DOI：10.1016/j.vetmic.2017.04.015.
[12] Moustafa AM, Bennett MD, Edwards J, et al. Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis[J]. Res Vet Sci, 2013, 95（3）：986-990. DOI：10.1016/j.rvsc.2013.07.003.
[13] Peng Z, Wang X, Zhou R, et al. Pasteurella multocida：Genotypes and genomics[J]. Microbiol Mol Biol Rev, 2019, 83（4）：e00014-e00019. DOI：10.1128/MMBR.00014-19.
[14] Soni S, Chahar M, Priyanka, et al. Identification of Aztreonam as a potential antibacterial agent against Pasteurella multocida sialic acid binding protein：A combined in silico and in-vitro analysis[J]. Microb Pathog, 2023, 185：106398. DOI：10.1016/j.micpath.2023.106398.
[15] Wu MC, Lo YT, Wu HC, et al. Cross-protection of recombinant Pasteurella multocida toxin proteins against atrophic rhinitis in mice[J]. Res Vet Sci, 2021, 137：138-143. DOI：10.1016/j.rvsc.2021.05.002.
[16] Tatum FM, Yersin AG, Briggs RE. Construction and virulence of a Pasteurella multocida fhaB2 mutant in turkeys[J]. Microb Pathog, 2005, 39（1-2）：9-17. DOI：10.1016/j.micpath.2005.05.003.
[17] Yu C, Sizhu S, Luo Q, et al. Genome sequencing of a virulent avian Pasteurella multocida strain GX-Pm reveals the candidate genes involved in the pathogenesis[J]. Res Vet Sci, 2016, 105：23-27. DOI：10.1016/j.rvsc.2016.01.013.
[18] Wilkie IW, Harper M, Boyce JD, et al. Pasteurella multocida：Diseases and pathogenesis[J]. Curr Top Microbiol Immunol, 2012, 361：1-22. DOI：10.1007/82_2012_216.
[19] Kim J, Kim JW, Oh SI, et al. Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea[J]. BMC Vet Res, 2019, 15（1）：119. DOI：10.1186/s12917-019-1861-5.
[20] Ziagham A, Gharibi D, Mosallanejad B, et al. Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates[J]. Vet Med Sci, 2024, 10（3）：e1424. DOI：10.1002/vms3.1424.
[21] Ujvári B, Weiczner R, Deim Z, et al. Characterization of Pasteurella multocida strains isolated from human infections[J]. Comp Immunol Microbiol Infect Dis, 2019, 63：37-43. DOI：10.1016/j.cimid.2018.12.008.
[22] Piorunek M, Brajer-Luftmann B, Walkowiak J. Pasteurella multocida infection in humans[J]. Pathogens, 2023, 12（10）：1210. DOI：10.3390/pathogens12101210.
[23] Harris PJ, Osswald MB. Pasteurella multocida epiglottitis：A review and report of a new case with associated chronic lymphocytic leukemia[J]. Ear Nose Throat J, 2010, 89（12）：E4. DOI：10.1177/014556131008901202.
[24] Wade T, Booy R, Teare EL, et al. Pasteurella multocida meningitis in infancy:A lick may be as bad as a bite[J]. Eur J Pediatr, 1999, 158（11）：875-878. DOI：10.1007/s004310051232.
[25] Kumar A, Devlin HR, Vellend H. Pasteurella multocida meningitis in an adult：Case report and review[J]. Rev Infect Dis, 1990, 12（3）：440-448. DOI：10.1093/clinids/12.3.440.
[26] Kobayaa H, Souki RR, Trust S, et al. Pasteurella multocida meningitis in newborns after incidental animal exposure[J]. Infect Dis J, 2009, 28（10）：928-929. DOI：10.1097/INF.0b013e3181a81f0f.
[27] Laupland KB, Stewart AG, Edwards F, et al. Pasteurella species bloodstream infections in Queensland, Australia, 2000-2019[J]. Eur J Clin Microbiol Infect Dis. 2022, 41（4）：609-614. DOI：10.1007/s10096-022-04411-w.
[28] Chatelier E, Mahieu R, Hamel JF, et al. Pasteurella bacteraemia：Impact of comorbidities on outcome, based on a case series and literature review[J]. Int J Infect Dis, 2020, 92：89-96. DOI：10.1016/j.ijid.2020.01.003.
[29] Piorunek M, Brajer-Luftmann B, Trafas T, et al. Lower respiratory infection in humans caused by Pasteurella multocida[J]. Respir Physiol Neurobiol, 2023, 315：104091. DOI：10.1016/j.resp.2023.104091.
[30] Kuechly H, Gupta R, Kurkowski S, et al. Culture negative Pasteurella multocida confirmed prosthetic hip infection using next-generation sequencing[J]. J Orthop Case Rep, 2024, 14（3）：50-54. DOI：10.13107/jocr.2024.v14.i03.4284.
[31] Honnorat E, Seng P, Savini H, et al. Prosthetic joint infection caused by Pasteurella multocida：A case series and review of literature[J]. BMC Infect Dis, 2016, 16（1）：435. DOI：10.1186/s12879-016-1763-0.
[32] Porter RS, Hay CM. Pasteurella endocarditis：A case report and statistical analysis of the literature[J]. Case Rep Infect Dis, 2020, 2020：8890211. DOI：10.1155/2020/8890211.
[33] Rachina SA, Mukhina NV, Cherkasova NA, et al. Unexpected clinical case of Pasteurella multocida infectious endocarditis in a patient with iv drug abuse：Why epidemiological history matters[J]. Germs, 2022, 12（1）：130-136. DOI：10.18683/germs.2022.1316.
[34] Hanson J, Etemady-Deylamy A, Frisby J, et al. Femoral artery aneurysm with large hematoma from Pasteurella：Case report and literature review[J]. BMC Infect Dis, 2022, 22（1）：170. DOI：10.1186/s12879-022-07136-5.
[35] Jeng EI, Acosta G, Martin TD, et al. Pasteurella multiocida infection resulting in a descending thoracic aorta mycotic pseudoaneurysm[J]. J Card Surg, 2020, 35（8）：2070-2072. DOI：10.1111/jocs.14776.
[36] Yang BF, Li NZ, Zou LX, et al. Identification and partial biological characteristics of six serotype A Pasteurella multocida from beef cattle[J]. Chin J Prev Vet Med, 2014, 36（6）：487-489. DOI：10.3969/j.issn.1008-0589.2014.06.17.（in Chinese）杨宝凤, 李能章, 邹灵秀, 等. 6株牛源A型多杀性巴氏杆菌的分离与鉴定[J]. 中国预防兽医学报, 2014, 36（6）：487-489. DOI：10.3969/j.issn.1008-0589.2014.06.17.
[37] Li N, Long Q, Du H, et al. High and low-virulent bovine Pasteurella multocida capsular type A isolates exhibit different virulence gene expression patterns in vitro and in vivo[J]. Vet Microbiol, 2016, 196：44-49. DOI：10.1016/j.vetmic.2016.10.017.
[38] Li JF, Yuan JL, Zhao Q, et al. Porcine cytotoxicity of recombinant Pasteurella multocida toxin and establishment of mouse （Mus musculus） pathological model [J]. J Agric Biotech, 2023, 31（1）：213-222. DOI：10.3969/j.issn.1674-7968.2023.01.021.（in Chinese）李金峰, 袁建林, 赵勤, 等. 重组多杀性巴氏杆菌毒素的猪细胞毒性及其小鼠病理模型的建立[J]. 农业生物技术学报, 2023, 31（1）：213-222. DOI：10.3969/j.issn.1674-7968.2023.01.021.
[39] Du QJ. Study on the dynamie variation in ducklings by artificially infected with Pasteurella multocida anatis [D]. Chengdu：Sichuan Agricultural University, 2007.（in Chinese）都启晶. 鸭源多杀性巴氏杆菌在人工感染雏鸭体内的动态研究[D]. 成都：四川农业大学, 2007.
[40] Mbuthia PG, Njagi LW, Nyaga PN, et al. Pasteurella multocida in scavenging family chickens and ducks：Carrier status, age susceptibility and transmission between species[J]. Avian Pathol, 2008, 37（1）：51-57. DOI：10.1080/03079450701784891.
[41] Wilson BA, Ho M. Pasteurella multocida：From zoonosis to cellular microbiology[J]. Clin Microbiol Rev, 2013, 26（3）：631-655. DOI：10.1128/CMR.00024-13.
[42] Bäumler A, Fang FC. Host specificity of bacterial pathogens[J]. Cold Spring Harb Perspect Med, 2013, 3（12）：a010041. DOI：10.1101/cshperspect.a010041.
[43] Peng Z, Liang W, Wang F, et al. Genetic and phylogenetic characteristics of Pasteurella multocida isolates from different host species[J]. Front Microbiol, 2018, 9：1408. DOI：10.3389/fmicb.2018.01408.
[44] E-Kobon T, Leeanan R, Pannoi S, et al. OmpA protein sequence-based typing and virulence-associated gene profiles of Pasteurella multocida isolates associated with bovine haemorrhagic septicaemia and porcine pneumonic pasteurellosis in Thailand[J]. BMC Vet Res, 2017, 13（1）：243. DOI：10.1186/s12917-017-1157-6.
[45] Nefedchenko AV, Glotova TI, Glotov AG, et al. Prevalence of different OmpH-types among Pasteurella multocida isolated from lungs of calves with respiratory problems[J]. Microb Pathog, 2017, 104：184-189. DOI：10.1016/j.micpath.2017.01.034.
[46] Fang C, Li RC, Wei R, et al. Identification, virulent factors detection and pathogenesis investigation of capsule serotype F Pasteurella multocida isolated from pigs[J]. Chin J Prev Vet Med, 2017, 39（3）：210-214. DOI：10.3969/j.issn.1008-0425.2017.03.10.（in Chinese）方超, 李润成, 魏榕, 等. 猪源荚膜血清F型多杀性巴氏杆菌的分离鉴定、毒力基因检测与致病性研究[J]. 中国预防兽医学报, 2017, 39（3）：210-214. DOI：10.3969/j.issn.1008-0425.2017.03.10.
[47] Sahoo M, Baloni S, Thakor JC, et al. Localization of Pasteurella multocida antigens in the brains of pigs naturally infected with pasteurellosis revealing a newer aspect of pathogenesis[J]. Microb Pathog, 2020, 140：103968. DOI：10.1016/j.micpath.2020.103968.
[48] Confer AW. Update on bacterial pathogenesis in BRD[J]. Anim Health Res Rev, 2009, 10（2）：145-148. DOI：10.1017/S1466252309990193.
[49] Shafarin MS, Zamri-Saad M, Khairani BS, et al. Pathological changes in the respiratory tract of goats infected by Pasteurella multocida B：2[J]. J Comp Pathol, 2009, 140（2-3）：194-197. DOI：10.1016/j.jcpa.2008.10.005.
[50] El-Demerdash AS, Mowafy RE, Fahmy HA, et al. Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt[J]. World J Microbiol Biotechnol, 2023, 39（12）：335. DOI：10.1007/s11274-023-03774-2.
[51] Kharb S, Charan S. Mouse model of haemorrhagic septicaemia：dissemination and multiplication of Pasteurella multocida B：2 in vital organs after intranasal and subcutaneous challenge in mice[J]. Vet Res Commun, 2013, 37（1）：59-63. DOI：10.1007/s11259-012-9547-5.
[52] Wu X, Zeng Z, Tian H, et al. The important role of NLRP6 inflammasome in Pasteurella multocida infection[J]. Vet Res, 2022, 53（1）：81. DOI：10.1186/s13567-022-01095-0.
[53] Mathy NL, Mathy JPD, Lee RP, et al. Pathological and immunological changes after challenge infection with Pasteurella multocida in naive and immunized calves[J]. Vet Immunol Immunopathol, 2002, 85（3-4）：179-188. DOI：10.1016/s0165-2427（01）00427-5.
[54] Pabst T, Joncourt R, Shumilov E, et al. Analysis of IL-6 serum levels and CAR T cell-specific digital PCR in the context of cytokine release syndrome[J]. Exp Hematol, 2020, 88：7-14.e3. DOI：10.1016/j.exphem.2020.07.003.
[55] Cron RQ, Goyal G, Chatham WW. Cytokine storm syndrome[J]. Annu Rev Med, 2023, 74：321-337. DOI：10.1146/annurev-med-042921-112837.
[56] Wang H, Wang LL, Zhao SJ, et al. IL-10：A bridge between immune cells and metabolism during pregnancy[J]. J Reprod Immunol, 2022, 154：103750. DOI：10.1016/j.jri.2022.103750.
[57] Wang F, Wang X, Ai W, et al. Three novel immunogenic proteins determined through 2-Dimensional electrophoresis and mass spectrometry with immune serum confer protection against challenge with porcine Pasteurella multocida in mouse models[J]. Res Vet Sci, 2021, 136：303-309. DOI：10.1016/j.rvsc.2021.03.013.
[58] Liu M, Huang HM, Wu JM, et al. Isolation and identification of rabbit's Pasteurella multocida and its antimicrobial resistance[J]. J South Agric, 2010, 41（11）：1219-1222. DOI：10.3969/j.issn.2095-1191.2010.11.020.（in Chinese）刘梅, 黄红梅, 吴健敏, 等. 兔多杀性巴氏杆菌的分离鉴定及耐药性研究[J]. 广西农业科学, 2010, 41（11）：1219-1222. DOI：10.3969/j.issn.2095-1191.2010.11.020.
[59] Roy Chowdhury P, Alhamami T, Venter H, et al. Identification and evolution of ICE-PmuST394：A novel integrative conjugative element in Pasteurella multocida ST394[J]. J Antimicrob Chemother, 2024, 79（4）：851-858. DOI：10.1093/jac/dkae040.
[60] Zhao G, Tang Y, Dan R, et al. Pasteurella multocida activates apoptosis via the FAK-AKT-FOXO1 axis to cause pulmonary integrity loss, bacteremia, and eventually a cytokine storm[J]. Vet Res, 2024, 55（1）：46. DOI：10.1186/s13567-024-01298-7.
[61] He F, Qiu Y, Wu X, et al. Slc6a13 deficiency attenuates Pasteurella multocida infection-induced inflammation via glycine-inflammasome signaling[J]. J Innate Immun, 2023, 15（1）：107-121. DOI：10.1159/000525089.
[62] Guo M, Zhang J, Li M, et al. NLRC3 negatively regulates Pasteurella multocida-induced NF-κB signaling in rabbits[J]. Dev Comp Immunol, 2024, 150：105078. DOI：10.1016/j.dci.2023.105078.
[63] Zhao G, Tang Y, Liu X, et al. Pasteurella multocida activates Rassf1-Hippo-Yap pathway to induce pulmonary epithelial apoptosis[J]. Vet Res, 2024, 55（1）：31. DOI：10.1186/s13567-024-01285-y.
[64] Sun J, Huang Y, Li X, et al. Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages[J]. Appl Microbiol Biotechnol, 2024, 108（1）：238. DOI：10.1007/s00253-024-13060-2.
[65] Thanasarasakulpong A, Poolperm P, Tangjitjaroen W, et al. Comparison of the effect of two purification methods on the immunogenicity of recombinant outer membrane protein H of Pasteurella multocida serovar A：1[J]. Vet Med Int, 2016, 2016：2579345. DOI：10.1155/2016/2579345.
[66] Zhou G, Tian J, Tian Y, et al. Recombinant-attenuated Salmonella enterica serovar choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge[J]. BMC Vet Res, 2023, 19（1）：128. DOI：10.1186/s12917-023-03679-0.
[67] Zhu W, Zhou B, Chen L, et al. Combinations but not a single PlpE epitope induces host protective immunity against Pasteurella multocida[J]. Infect Immun, 2023, 91（3）：e0027222. DOI：10.1128/iai.00272-22.
[68] Chen N, Jiang D, Liu Y, et al. Preparation of Escherichia coli ghost of anchoring bovine Pasteurella multocida OmpH and its immunoprotective effect[J]. BMC Vet Res, 2023, 19（1）：192. DOI：10.1186/s12917-023-03743-9.
[69] Wang Z, Wang M, Wang F, et al. Dendritic cell targeting peptide plus Salmonella FliCd flagellin fused outer membrane protein H （OmpH） demonstrated increased efficacy against infections caused by different Pasteurella multocida serogroups in mouse models[J]. Vaccine, 2024, 42（12）：3075-3083. DOI：10.1016/j.vaccine.2024.04.020.
[70] Yang X, Fu Q, Zhang W, et al. Overexpression of Pasteurella multocida OmpA induces transcriptional changes and its possible implications for the macrophage polarization[J]. Microb Pathog, 2023, 183：106212. DOI：10.1016/j.micpath.2023.106212.
[71] Lv Q, Shang Y, Bi H, et al. Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis[J]. Microbes Infect, 2023：105235. DOI：10.1016/j.micinf.2023.105235.
[72] Wang Z, Li L, Liu P, et al. Role of aspartate ammonia-lyase in Pasteurella multocida[J]. BMC Microbiol, 2020, 20（1）：369. DOI：10.1186/s12866-020-02049-2