2022—2023年三峡库区重庆段小兽携带鼠传病原体现况调查

doi:10.11853/j.issn.1003.8280.2024.06.019

中国媒介生物学及控制杂志 ›› 2024, Vol. 35 ›› Issue (6): 728-733.DOI: 10.11853/j.issn.1003.8280.2024.06.019

2022—2023年三峡库区重庆段小兽携带鼠传病原体现况调查

王玲^1,2, 肖汉森^1,2, 何亚明^1,2, 赵婷^1,2, 李志峰^1,2, 黄为^1,2

1. 重庆市疾病预防控制中心微生物检验所/消毒与媒介生物控制所, 重庆 400707;
2. 重庆市高致病性病原微生物重点实验室, 重庆 400707

收稿日期:2024-01-05 出版日期:2024-12-20 发布日期:2024-12-20
通讯作者: 黄为,E-mail:hw827@163.com
作者简介:王玲,女,副主任技师,研究方向为病原微生物检验,E-mail:787704919@qq.com
基金资助:
重庆市自然科学基金面上项目（CSTB2022 NSCQ-MSX0572）；重庆市英才计划（cstc2022ycjh-bgzxm0251）；重庆市科卫联合医学科研项目（2022MSXM078）

An investigation of rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area, China, 2022-2023

WANG Ling^1,2, XIAO Han-sen^1,2, HE Ya-ming^1,2, ZHAO Ting^1,2, LI Zhi-feng^1,2, HUANG Wei^1,2

1. Department of Microbiological Detection/Department of Disinfection and Vector Control, Chongqing Center for Disease Control and Prevention, Chongqing 400707, China;
2. Chongqing Municipal Key Laboratory for High Pathogenic Microbes, Chongqing 400707, China

Received:2024-01-05 Online:2024-12-20 Published:2024-12-20
Supported by:
Chongqing Natural Science Foundation General Project (No. CSTB2022 NSCQ-MSX0572);Chongqing Talent Plan (No. cstc2022ycjh-bgzxm0251);Chongqing Science and Health Joint Medical Research Foundation (No. 2022MSXM078)

摘要/Abstract

摘要： 目的调查三峡库区重庆段小兽5种鼠传病原体的携带情况,为库区鼠源疾病防控提供科学指导。方法 2022－2023年,在三峡库区重庆段居住区、耕作地及重点行业3类生境使用夹（笼）夜法捕获小型兽类,提取肝、脾、肾组织的核酸样本,采用实时荧光定量PCR（qPCR）检测鼠疫耶尔森菌、巴尔通体、伯氏疏螺旋体、钩端螺旋体（钩体）、恙虫病东方体；采集心脏血,采用血清学方法检测血清中鼠疫F1抗体、伯氏疏螺旋体IgG抗体、钩体血清抗体。运用Excel 2016和SPSS 22.0软件进行数据整理分析,病原体感染率的比较采用χ²检验或Fisher确切概率法进行统计分析。结果共捕获小型兽类10种361只,黄胸鼠和褐家鼠为优势鼠种,占比分别为53.46%和12.47%。动物肝、肾、脾组织检测结果显示,钩体阳性9份（2.50%）、巴尔通体阳性17份（4.71%）,未检出鼠疫耶尔森菌、伯氏疏螺旋体、恙虫病东方体。小兽病原体总感染率为6.65%；黄胸鼠存在钩体和巴尔通体复合感染,复合感染率为0.55%。动物血清抗体检测结果显示,伯氏疏螺旋体IgG抗体阳性2份（0.80%）,钩体血清抗体阳性4份（1.59%）；鼠疫F1抗体均为阴性。结论三峡库区重庆段小兽存在钩体、巴尔通体、伯氏疏螺旋体的感染,应加强三峡库区重庆段小兽携带鼠传病原体监测,积极开展鼠类防控工作,保障当地人群公共健康。

关键词: 三峡库区, 小型兽类, 钩端螺旋体, 巴尔通体, 伯氏疏螺旋体

Abstract: Objective To investigate the prevalence of five rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area, China, so as to provide scientific guidance for the prevention and control of rodent-borne diseases in the region. Methods From 2022 to 2023, small mammals were captured using the night snap (cage) trapping method in residential areas, cultivated lands, and key industries in the Chongqing section of the Three Gorges Reservoir area. Liver, spleen, and kidney tissues were collected to extract nucleic acids for the detection of Yersinia pestis, Bartonella, Borrelia burgdorferi, Leptospira, and Orientia tsutsugamushi using quantitative real-time PCR (qPCR). Blood samples from the heart were collected to detect Y.pestis F1 antibody, B. burgdorferi IgG antibody, and Leptospira serum antibody using serological methods. Excel 2016 and the SPSS 22.0 softwares were used for data processing and analysis. The pathogen infection rates of small mammals were compared using the Chi-square test or Fisher's exact test. Results A total of 361 small mammals belonging to 10 species were captured, with Rattus tanezumi and R. norvegicus being the dominant species, accounting for 53.46% and 12.47%, respectively. Detection of liver, spleen, and kidney tissues showed that 9 samples (2.50%) were positive for Leptospira and 17 samples (4.71%) were positive for Bartonella. No samples tested positive for Y. pestis, B. burgdorferi, or O. tsutsugamushi. The overall pathogen infection rates of small mammals were 6.65%. Co-infection of Leptospira and Bartonella was detected in R. tanezumi, and the co-infection rate was 0.55%. Animal serum antibody test results showed that 2 samples (0.80%) were positive for B. burgdorferi IgG antibody, 4 samples (1.59%) were positive for Leptospira antibody, and no samples were positive for Y. pestis F1 antibody. Conclusions Small mammals in the Chongqing section of the Three Gorges Reservoir area carry a variety of pathogens including Leptospira, Bartonella, and B. burgdorferi. Surveillance of rodent-borne pathogens carried by small mammals in this region should be enhanced and rodent prevention and control measures should be actively implemented to safeguard the public health of local residents.

Key words: Three Gorges Reservoir area, Small mammal, Leptospira, Bartonella, Borrelia burgdorferi

中图分类号:

R183
R37

王玲, 肖汉森, 何亚明, 赵婷, 李志峰, 黄为. 2022—2023年三峡库区重庆段小兽携带鼠传病原体现况调查[J]. 中国媒介生物学及控制杂志, 2024, 35(6): 728-733.

WANG Ling, XIAO Han-sen, HE Ya-ming, ZHAO Ting, LI Zhi-feng, HUANG Wei. An investigation of rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area, China, 2022-2023[J]. Chinese Journal of Vector Biology and Control, 2024, 35(6): 728-733.

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2022—2023年三峡库区重庆段小兽携带鼠传病原体现况调查

An investigation of rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area, China, 2022-2023

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