内蒙古长爪沙鼠鼠疫自然疫源地巴尔通体感染情况调查

doi:10.11853/j.issn.1003.8280.2024.04.021

中国媒介生物学及控制杂志 ›› 2024, Vol. 35 ›› Issue (4): 500-507.DOI: 10.11853/j.issn.1003.8280.2024.04.021

内蒙古长爪沙鼠鼠疫自然疫源地巴尔通体感染情况调查

刘洋¹, 李建云², 刘芳², 张喆¹, 张娇¹

1. 包头医学院公共卫生学院, 内蒙古包头 014000;
2. 内蒙古自治区疾病预防控制中心鼠疫防控科, 内蒙古呼和浩特 010000

收稿日期:2024-03-11 出版日期:2024-08-20 发布日期:2024-08-27
通讯作者: 李建云,E-mail:hhhtlijianyun@163.com
作者简介:刘洋,男,在读硕士,主要从事鼠传病原体的研究工作,E-mail:1481257952@qq.com
基金资助:
内蒙古自治区鼠疫防控关键技术研究(2021ZD0006);二连浩特地区重点鼠传疾病和鼠类DNA条形码的调查研究(2018MS08059);国家重点研发计划项目(2021YFC1200200)

An investigation of Bartonella infections in the natural foci of Meriones unguiculatus plague in Inner Mongolia Autonomous Region, China

LIU Yang¹, LI Jian-yun², LIU Fang², ZHANG Zhe¹, ZHANG Jiao¹

1. School of Public Health, Baotou Medical College, Baotou, Inner Mongolia 014000, China;
2. Department of Plague Prevention and Control, Inner Mongolia Autonomous Region Center for Disease Control and Prevention, Hohhot, Inner Mongolia 010000, China

Received:2024-03-11 Online:2024-08-20 Published:2024-08-27
Supported by:
Research on Key Technologies for Plague Prevention and Control in Inner Mongolia Autonomous Region (No. 2021ZD0006); Investigation and Research on Key Rodent-borne Diseases and Rodent DNA Barcoding in Erenhot Area (No. 2018MS08059); National Key R&D Program of China (No. 2021YFC1200200)

摘要/Abstract

摘要： 目的调查巴尔通体在内蒙古自治区（内蒙古）长爪沙鼠鼠疫自然疫源地鼠类动物种群中的分布及流行特征,并研究巴尔通体的遗传多样性及其系统发育进化关系,为预防控制巴尔通体的人间感染提供基础数据。方法在内蒙古乌兰察布市四子王旗和杭锦旗郊区采用夹夜法采集鼠类,核酸提取试剂盒提取鼠类啮齿动物肝脏组织样本DNA,应用PCR对样本核酸gltA、ftsZ、ITS基因进行检测,电泳阳性PCR产物测序,获得序列使用MEGA 11.0软件进行序列分析和构建系统进化树,分析确定病原体的种类。Fisher确切概率检验分析不同鼠种巴尔通体感染率的差异。结果 2021—2022年,共采集鼠类175只,形态学鉴定为7种。经gltA基因检测,巴尔通体阳性率为42.86%（75/175）。内蒙古长爪沙鼠鼠疫疫源地鼠类中携带的巴尔通体为沙鼠巴尔通体（Bartonella gerbillinarum）、念珠菌巴尔通体（Candidatus B. negeviensis）、克拉斯诺夫巴尔通体（B. krasnovii）、格拉汉姆巴尔通体（B. grahamii）、汉赛巴尔通体（B. henselae）和B. washoensis,共计6种。长爪沙鼠、达乌尔黄鼠、黄兔尾鼠、黑线仓鼠、五趾跳鼠、子午沙鼠等6种检出阳性的鼠类间巴尔通体感染率差异有统计学意义（P=0.001）。结论此次研究检测到的巴尔通体阳性率较高,物种丰富,包含可感染人的格拉汉姆巴尔通体和汉赛巴尔通体,且存在感染宿主特异性。

关键词: 巴尔通体, 内蒙古, 遗传进化, 鼠类

Abstract: Objective To investigate the distribution and epizootic characteristics of Bartonella in rodents population at the natural foci of Meriones unguiculatus plague in the Inner Mongolia Autonomous Region (Inner Mongolia), China, and study the genetic diversity and evolutionary relationship of Bartonella, so as to provide basic data for the prevention and control of Bartonella infection in humans. Methods In the suburbs of Siziwang Banner and Hangjin Banner of Ulanqab, Inner Mongolia, rodents were collected by night snap trapping. DNA was extracted from rodent liver tissue samples using nucleic acid extraction kits. Polymerase chain reaction (PCR) was used to detect the gltA, ftsZ, and ITS genes. The positive PCR products were sequenced for sequence analysis and phylogenetic analysis for pathogen identification with MEGA 11.0 software. The Fisher's exact test was used to compare the Bartonella infection rates of different rodent species. Results From 2021 to 2022, a total of 175 rodents of seven species (by morphological identification) were collected. The positive rate of Bartonella by gltA gene detection was 42.86% (75/175). These rodents carried six species of Bartonella: B. gerbillinarum, Candidatus B. negeviensis, B. krasnovii, B. grahamii, B. henselae, and B. washoensis. The Bartonella infection rate differed significantly between the 6 species of rodents of M. unguiculatus, Citellus dauricus, Eolagurus luteus, Cricetulus barabensis, Allactaga sibirica, and M. meridianus (P=0.001). Conclusion Bartonella infections are prevalent in this study area, involving a variety of species such as B. grahamii and B. henselae that can infect humans and have host specificity.

Key words: Bartonella, Inner Mongolia, Genetic evolution, Rodent

中图分类号:

R183

刘洋, 李建云, 刘芳, 张喆, 张娇. 内蒙古长爪沙鼠鼠疫自然疫源地巴尔通体感染情况调查[J]. 中国媒介生物学及控制杂志, 2024, 35(4): 500-507.

LIU Yang, LI Jian-yun, LIU Fang, ZHANG Zhe, ZHANG Jiao. An investigation of Bartonella infections in the natural foci of Meriones unguiculatus plague in Inner Mongolia Autonomous Region, China[J]. Chinese Journal of Vector Biology and Control, 2024, 35(4): 500-507.

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内蒙古长爪沙鼠鼠疫自然疫源地巴尔通体感染情况调查

An investigation of Bartonella infections in the natural foci of Meriones unguiculatus plague in Inner Mongolia Autonomous Region, China

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