湖南省长沙市内五区白纹伊蚊电压门控钠离子通道基因突变研究

doi:10.11853/j.issn.1003.8280.2024.04.004

中国媒介生物学及控制杂志 ›› 2024, Vol. 35 ›› Issue (4): 405-410.DOI: 10.11853/j.issn.1003.8280.2024.04.004

湖南省长沙市内五区白纹伊蚊电压门控钠离子通道基因突变研究

朱彩英¹, 赵春春², 王纯玉³, 陈建勇¹, 肖珊¹, 王文强¹, 孟凤霞²

1. 长沙市疾病预防控制中心消毒与病媒生物防制科, 湖南长沙 410000;
2. 传染病溯源预警与智能决策全国重点实验室, 中国疾病预防控制中心传染病预防控制所媒介生物控制室, WHO媒介生物监测与管理合作中心, 北京 102206;
3. 辽宁省疾病预防控制中心, 辽宁沈阳 110000

收稿日期:2023-11-01 出版日期:2024-08-20 发布日期:2024-08-27
通讯作者: 孟凤霞,E-mail:mengfengxia@icdc.cn
作者简介:朱彩英,女,硕士,医师,主要从事病媒生物防制研究,E-mail:zhucaiying_ivy@163.com
基金资助:
湖南省卫生健康委科研计划课题(202212053446)

A study on voltage-gated sodium channel gene mutations in Aedes albopictus in five urban districts of Changsha, Hunan Province, China

ZHU Cai-ying¹, ZHAO Chun-chun², WANG Chun-yu³, CHEN Jian-yong¹, XIAO Shan¹, WANG Wen-qiang¹, MENG Feng-xia²

1. Department of Disinfection and Vector Control, Changsha Center for Disease Control and Prevention, Changsha, Hunan 410000, China;
2. Department of Vector Biology and Control, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
3. Liaoning Center for Disease Control and Prevention, Shenyang, Liaoning 110000, China

Received:2023-11-01 Online:2024-08-20 Published:2024-08-27
Supported by:
Scientific Research Project of Hunan Provincial Health Commission (No. 202212053446)

摘要/Abstract

摘要： 目的检测长沙市内五区登革热媒介白纹伊蚊野外种群电压门控钠离子通道（VGSC）基因的基因型及分布特点,了解其击倒抗性情况,为白纹伊蚊科学防控提供依据。方法 2023年6—7月在长沙市内五区采集白纹伊蚊成蚊,经形态学鉴定后,用75%乙醇溶液浸泡,并于-20 ℃冻存备用。对白纹伊蚊单只样本提取基因组DNA,扩增VGSC基因目的片段,对目的片段进行测序分析,确定VGSC基因V1016、I1532和F1534单位点突变及多位点组合突变情况。结果共采集白纹伊蚊成蚊样本184份（只）,3个位点均测序成功的样本共计180份（只）。检测发现VGSC基因V1016与F1534位点存在突变。V1016位点有2种等位基因,即野生型GTA/V（占比74.72%）和突变型GGA/G（25.28%）；有3种基因型,分别是野生型纯合子V/V（58.33%）、野生/突变型杂合子V/G（32.78%）和突变型纯合子G/G（8.89%）。I1532位点未发现突变个体,只检测到1种等位基因和基因型,即野生型ACC/I（100%）和野生型纯合子I/I（100%）。F1534位点检测到3种等位基因,为野生型TTC/F（23.62%）、突变型TCC/S（39.44%）和TGC/C（36.94%）；有6种基因型,即野生型纯合子F/F（6.67%）、野生/突变型杂合子F/S（21.67%）和F/C（12.22%）,突变型纯合子S/S（14.44%）和C/C（16.67%）,突变型杂合子S/C（28.33%）。结论长沙市中心城区白纹伊蚊VGSC基因V1016和F1534位点突变率高且突变基因型丰富多样,从分子层面说明其对拟除虫菊酯类杀虫剂已产生抗药性,提示应科学合理使用杀虫剂,并加强抗药性监测,从而延缓抗药性发展。

关键词: 白纹伊蚊, 拟除虫菊酯类杀虫剂, 击倒抗性, 长沙市

Abstract: Objective To investigate the genotypes of the voltage-gated sodium channel (VGSC) gene and their distribution in field populations of Aedes albopictus in the "inner five districts" of Changsha, Hunan Province, China for information on their knockdown resistance levels, so as to provide a basis for scientific control of Ae. albopictus. Methods Adult Ae. albopictus mosquitoes were collected from five districts in the urban area of Changsha from June to July 2023, and then were soaked in 75% ethanol and stored at -20 ℃ after morphological identification. The genomic DNA of every single mosquito was extracted to amplify and sequence the target fragment of the VGSC gene to determine single-locus and multi-locus mutations at V1016, I1532, and F1534 of the VGSC gene. Results A total of 184 Ae. albopictus mosquitoes were collected, and the three loci were all successfully sequenced for 180 of the 184 samples. Mutations were detected at the V1016 and F1534 loci. At V1016, two alleles were detected (wild-type GTA/V, 74.72%; and mutant GGA/G, 25.28%); and three genotypes were found (wild-type homozygote V/V, 58.33%; wild/mutant heterozygote V/G, 32.78%; and mutant homozygote G/G, 8.89%). No mutant individuals were found at the I1532 locus, and only one allele (wild-type ACC/I, 100%) and one genotype (wild-type homozygote I/I, 100%) were detected. At F1534, there were three alleles (wild-type TTC/F, 23.62%; mutant TCC/S, 39.44%; and mutant TGC/C, 36.94%) and six genotypes [wild-type homozygote F/F (6.67%); wild/mutant heterozygotes F/S (21.67%) and F/C (12.22%); mutant homozygotes S/S (14.44%) and C/C (16.67%); and mutant heterozygote S/C (28.33%)]. Conclusions Ae. albopictus mosquitoes in central urban districts of Changsha harbor frequent mutations and diverse genotypes at V1016 and F1534 in the VGSC gene, indicating the presence of resistance to pyrethroids at the molecular level, highlighting the necessity of scientific and reasonable use of insecticides and intensive resistance surveillance to delay the development of insecticide resistance.

Key words: Aedes albopictus, Pyrethroids, Knockdown resistance, Changsha

中图分类号:

朱彩英, 赵春春, 王纯玉, 陈建勇, 肖珊, 王文强, 孟凤霞. 湖南省长沙市内五区白纹伊蚊电压门控钠离子通道基因突变研究[J]. 中国媒介生物学及控制杂志, 2024, 35(4): 405-410.

ZHU Cai-ying, ZHAO Chun-chun, WANG Chun-yu, CHEN Jian-yong, XIAO Shan, WANG Wen-qiang, MENG Feng-xia. A study on voltage-gated sodium channel gene mutations in Aedes albopictus in five urban districts of Changsha, Hunan Province, China[J]. Chinese Journal of Vector Biology and Control, 2024, 35(4): 405-410.

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湖南省长沙市内五区白纹伊蚊电压门控钠离子通道基因突变研究

A study on voltage-gated sodium channel gene mutations in Aedes albopictus in five urban districts of Changsha, Hunan Province, China

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