东北部分地区蜱携带病原体的调查研究

doi:10.11853/j.issn.1003.8280.2024.03.020

摘要/Abstract

摘要： 目的了解东北3省蜱类携带病原体情况,为蜱传疾病的防控提供参考依据。方法 2012-2013年、2016年在东北11个市（县）采集蜱标本,采用聚合酶链式反应（PCR）法检测斑点热群立克次体（SFGR）和巴尔通体,采用巢式PCR法检测新埃立克体、查菲埃立克体和土拉弗朗西斯菌（土拉菌）,采用实时荧光PCR法检测伯氏疏螺旋体和微小巴贝虫。结果 SFGR、查菲埃立克体、土拉菌、伯氏疏螺旋体和微小巴贝虫检出率分别为9.10%、4.10%、0.70%、14.95%和1.87%,差异有统计学意义（χ²=42.808,P<0.001）,伯氏疏螺旋体和SFGR检出率较高,未检出新埃立克体和巴尔通体。不同蜱种中SFGR、伯氏疏螺旋体及微小巴贝虫的阳性率差异有统计学意义（χ²=12.291,P=0.016；χ²=23.409,P<0.001；χ²=11.592,P=0.028）。结论东北地区蜱类携带多种病原体,以伯氏疏螺旋体、SFGR感染较普遍,不同蜱种携带病原体种类有差异,提示该地区应加强蜱媒病原体监测及调查,为蜱媒传染病的预防与控制提供依据。

关键词: 蜱, 蜱媒病原体, 聚合酶链式反应, 东北地区

Abstract: Objective To investigate the pathogens carried by ticks in three northeastern provinces of China and provide reference basis for the prevention and control of tick borne diseases.Methods From 2012 to 2013 and in 2016, tick specimens were collected from 11 counties and cities of northeastern China. Spotted fever group rickettsiae (SFGR) and Bartonella by PCR were tested, Candidatus Neoehrlichia mikurensis, Ehrlichia chaffeensis, and Francisella tularensis by nested PCR, and Borrelia burgdorferi and Babesia microti by quantitative real-time PCR.Results The detection rate was 9.10% for SFGR, 4.10% for E. chaffeensis, 0.70% for F. tularensis, 14.95% for Bor. burgdorferi, and 1.87% for Bab. microti, with a significant difference (χ²=42.808, P<0.001). Bor. burgdorferi and SFGR were most frequently detected, while Candidatus Neoehrlichia mikurensis and Bartonella were not found. There were significant differences between tick species in the positive rates of SFGR (χ²=12.291, P=0.016), Bor. burgdorferi (χ²=23.409, P<0.001), and Bab. microti (χ²=11.592, P=0.028).Conclusions Ticks carry a variety of pathogens in northeast China, in which Bor. burgdorferi and SFGR are more common, and pathogen species differ between tick species, suggesting that these areas should strengthen tick-borne pathogen surveillance to inform the prevention and control of tick-borne diseases.

Key words: Tick, Tick-borne pathogen, Polymerase chain reaction, Northeast China

中图分类号:

黄冠鹏, 吴琼, 马悠馨, 毛恩荣, 黄潇杨, 王卓, 赵洪明, 冷国双, 李兵, 王猷金, 吴益民. 东北部分地区蜱携带病原体的调查研究[J]. 中国媒介生物学及控制杂志, 2024, 35(3): 369-373.

HUANG Guan-peng, WU Qiong, MA You-xin, MAO En-rong, HUANG Xiao-yang, WANG Zhuo, ZHAO Hong-ming, LENG Guo-shuang, LI Bing, WANG You-jin, WU Yi-min. An investigation of tick-borne pathogens in parts of northeast China[J]. Chinese Journal of Vector Biology and Control, 2024, 35(3): 369-373.

参考文献

[1] Boulanger N,Boyer P,Talagrand-Reboul E,et al. Ticks and tick-borne diseases[J]. Med Mal Infect,2019,49（2）：87-97. DOI：10.1016/j.medmal.2019.01.007.
[2] Chen Z,Yang XJ. Systematics and taxonomy of Ixodida[M]. Beijing： Science Press, 2021：126. （in Chinese）陈泽,杨晓军. 蜱的系统分类学[M]. 北京：科学出版社,2021：126.
[3] Parola P,Paddock CD,Raoult D. Tick-borne rickettsioses around the world：Emerging diseases challenging old concepts[J]. Clin Microbiol Rev,2005,18（4）：719-756. DOI：10.1128/CMR.18. 4.719-756.2005.
[4] Zhou MH,Chen HN. A review on emerging tick-borne pathogens in China[J]. Chin J Hyg Insect Equip,2019,25（3）：193-198. DOI：10.19821/j.1671-2781.2019.03.001.（in Chinese）周明浩,陈红娜. 我国新发蜱媒病原体研究概述[J]. 中华卫生杀虫药械,2019,25（3）：193-198. DOI：10.19821/j.1671-2781. 2019.03.001.
[5] Fang LQ,Liu K,Li XL,et al. Emerging tick-borne infections in Chinese mainland：An increasing public health threat[J]. Lancet Infect Dis,2015,15（12）：1467-1479. DOI：10.1016/s1473-3099（15）00177-2.
[6] Paziewska A,Harris PD,Zwolińska L,et al. Recombination within and between species of the Alpha proteobacterium Bartonella infecting rodents[J]. Microb Ecol,2011,61（1）：134-145. DOI：10.1007/s00248-010-9735-1.
[7] Li H,Jiang JF,Tang F,et al. Wide distribution and genetic diversity of “Candidatus Neoehrlichia mikurensis” in rodents from China[J]. Appl Environ Microbiol,2013,79（3）：1024-1027. DOI：10.1128/AEM.02917-12.
[8] Zhang XC,Zhang LX,Li WH,et al. Ehrlichiosis and zoonotic anaplasmosis in suburban areas of Beijing,China[J]. Vector Borne Zoonotic Dis,2012,12（11）：932-937. DOI：10.1089/vbz.2012.0961.
[9] Fulop M,Leslie D,Titball R. A rapid,highly sensitive method for the detection of Francisella tularensis in clinical samples using the polymerase chain reaction[J]. Am J Trop Med Hyg,1996,54（4）：364-366. DOI：10.4269/ajtmh.1996.54.364.
[10] Wang Z,Wang JW,Yu M,et al. Investigation on Candidatus Neoehrlichia mikurensis in rodents collected in forest area in the northeastern region of China[J]. Chin J Zoonoses,2019,35（4）：330-333. DOI：10.3969/j.issn.1002-2694.2019.00.032.（in Chinese）王卓,王建伟,于淼,等. 东北边境地区啮齿动物中新埃立克体感染调查及groEL基因序列分析[J]. 中国人兽共患病学报,2019,35（4）：330-333. DOI：10.3969/j.issn.1002-2694.2019. 00.032.
[11] Huang GP,Wu Q,Wang Z,et al. Babesia microti infestation in rodents and ticks collected from northeastern China[J]. Chin J Hyg Insect Equip,2023,29（3）：242-244. DOI：10.19821/j.1671-2781.2023.03.014.（in Chinese）黄冠鹏,吴琼,王卓,等. 东北部分地区啮齿动物和蜱中微小巴贝斯虫感染调查[J]. 中华卫生杀虫药械,2023,29（3）：242-244. DOI：10.19821/j.1671-2781.2023.03.014.
[12] Wang Z,Wu Q,Jin MS,et al. An investigation of Bartonella infection in rodents in the Changbai Mountain in China and its genotype analysis[J]. Chin J Vector Biol Control,2021,32（5）：594-598. DOI：10.11853/j.issn.1003.8280.2021.05.017.（in Chinese）王卓,吴琼,金美松,等. 长白山地区野生啮齿动物巴尔通体感染调查及基因型分析[J]. 中国媒介生物学和控制杂志,2021,32（5）：594-598. DOI：10.11853/j.issn.1003.8280.2021. 05.017.
[13] Li JX,Zhao X,Jin GX,et al. Investigation of free ticks carrying CRT and compound infection with SFTSV in Yanbian area of Jilin Province[J]. Chin J Dis Control Prev,2019,23（5）：561-566,572. DOI：10.16462/j.cnki.zhjbkz.2019.05.014.（in Chinese）李基旭,赵鑫,金光星,等. 吉林延边地区蜱复合感染新塔拉塞维奇立克次体及SFTSV调查研究[J]. 中华疾病控制杂志,2019,23（5）：561-566,572. DOI：10.16462/j.cnki.zhjbkz.2019. 05.014.
[14] Wu YM,Zhang ZQ,Wang HJ,et al. Investigation on the epidemiology of far-east tick-borne spotted fever in the northeastern area of China[J]. Chin J Epidemiol,2008,29（12）：1173-1175. DOI：10.3321/j.issn：0254-6450.2008.12.001.（in Chinese）吴益民,张志强,王洪军,等. 中国东北地区远东蜱传斑点热流行病学调查[J]. 中华流行病学杂志,2008,29（12）：1173-1175. DOI：10.3321/j.issn：0254-6450.2008.12.001.
[15] Zhang AQ,Shen ZY,Lei YR,et al. Research progress on epidemiology of Lyme disease[J]. Chin J Hyg Insect Equip,2020,26（1）：79-83. DOI：10.19821/j.1671-2781.2020.01.020.（in Chinese）张爱勤,沈兆媛,雷韫睿,等. 莱姆病流行病学及其防治研究现状[J]. 中华卫生杀虫药械,2020,26（1）：79-83. DOI：10. 19821/j.1671-2781.2020.01.020.
[16] Li H,Jiang JF,Liu W,et al. Human infection with Candidatus Neoehrlichia mikurensis,China[J]. Emerg Infect Dis,2012,18（10）：1636-1639. DOI：10.3201/eid1810.120594.
[17] Jiang JF,Zheng YC,Jiang RR,et al. Epidemiological,clinical,and laboratory characteristics of 48 cases of “Babesia venatorum” infection in China：A descriptive study[J]. Lancet Infect Dis,2015,15（2）：196-203. DOI：10.1016/S1473-3099（14）71046-1.
[18] Shen B,Li JX,Huang B,et al. An investigation of tick species in Changbai Mountain area of Jilin Province,China[J]. Chin J Vector Biol Control,2020,31（6）：667-671. DOI：10.11853/j.issn.1003.8280.2020.06.008.（in Chinese）沈博,李基旭,黄飚,等. 吉林省长白山区蜱类调查研究[J]. 中国媒介生物学及控制杂志,2020,31（6）：667-671. DOI：10.11853/j.issn.1003.8280.2020.06.008.
[19] Wang Z,Wang JW,Yu M,et al. Molecular epidemiological studies on spotted fever group rickettsiae in ticks from Northeastern,China[J]. Chin J Vector Biol Control,2018,29（4）：344-347. DOI：10.11853/j.issn.1003.8280.2018.04.005.（in Chinese）王卓,王建伟,于淼,等. 东北地区蜱传斑点热群立克次体的分子流行病学研究[J]. 中国媒介生物学及控制杂志,2018,29（4）：344-347. DOI：10.11853/j.issn.1003.8280.2018.04.005.
[20] Fan DH,Li M,Xu HF,et al. The situation of mice and ticks infected by Babesia microtic[J]. Chin J Hyg Insect Equip,2012,18（1）：48-50. DOI：10.19821/j.1671-2781.2012.01.020.（in Chinese）范东辉,李明,徐翮飞,等. 鼠与蜱感染人致病性巴贝虫状况的初步研究[J]. 中华卫生杀虫药械,2012,18（1）：48-50. DOI：10.19821/j.1671-2781.2012.01.020.
[21] Wang YH,Mao LL,Peng Y,et al. Francisella tularensis was detected in Haemaphysalis longicornis from Liaoning Province[J]. Chin J Hyg Insect Equip,2016,27（6）：529-532. DOI：10.11853/j.issn.1003.8280.2016.06.001.（in Chinese）王艳华,毛玲玲,彭遥,等. 在辽宁省长角血蜱中检测出土拉弗朗西斯菌[J]. 中国媒介生物学及控制杂志,2016,27（6）：529-532. DOI：10.11853/j.issn.1003.8280.2016.06.001.
[22] Dong X,Chen XP,Liu N,et al. Co-circulation of multiple species of Rickettsiales bacteria in one single species of hard ticks in Shenyang,China[J]. Ticks Tick Borne Dis,2014,5（6）：727-733. DOI：10.1016/j.ttbdis.2014.05.011.
[23] Wang QY,Liu FM,Fu SH,et al. Investigation of tick-borne pathogens in Heilongjiang border area[J]. Chin J Exp Clin Virol,2019,33（5）：504-508. DOI：10.3760/cma.j.issn.1003-9279. 2019.05.013.（in Chinese）王倩莹,刘凤明,付士红,等. 黑龙江边境地区蜱虫携带病原体调查[J]. 中华实验和临床病毒学杂志,2019,33（5）：504-508. DOI：10.3760/cma.j.issn.1003-9279.2019.05.013.
[24] Rar VA,Livanova NN,Panov VV,et al. Genetic diversity of Anaplasma and Ehrlichia in the Asian part of Russia[J]. Ticks Tick Borne Dis,2010,1（1）：57-65. DOI：10.1016/j.ttbdis.2010. 01.002.
[25] Karnath C,Obiegala A,Speck S,et al. Detection of Babesia venatorum,Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis in Ixodes persulcatus ticks from Mongolia[J]. Ticks Tick Borne Dis,2016,7（2）：357-360. DOI：10.1016/j.ttbdis.2015.12.007.
[26] Ju WD,Wang YM,Xu N,et al. Investigation on Bartonella infection in rodents and ticks at Heihe airport in Heilongjiang Province[J]. Port Health Control,2018,23（2）：60-63. DOI：10.3969/j.issn.1008-5777.2018.02.019.（in Chinese）鞠文东,王艳梅,徐宁,等. 黑龙江黑河口岸鼠类及蜱类携带巴尔通体的调查[J]. 口岸卫生控制,2018,23（2）：60-63. DOI：10.3969/j.issn.1008-5777.2018.02.019.
[27] Yin XP,Zhao SS,Tiao YH,et al. Detection of Bartonella from rodent-harboring fleas and free-living ticks at Alashankou pass,China-Kazakhstan border[J]. Chin J Vector Biol Control,2017,28（4）：350-353. DOI：10.11853/j.issn.1003.8280.2017.04.011.（in Chinese）尹小平,赵姗姗,田延河,等. 中国-哈萨克斯坦边境阿拉山口口岸鼠体蚤及游离蜱感染巴尔通体调查[J]. 中国媒介生物学及控制杂志,2017,28（4）：350-353. DOI：10.11853/j.issn. 1003.8280.2017.04.011.