山西省长治市宠物犬人兽共患病病原菌及巴贝虫感染状况调查

doi:10.11853/j.issn.1003.8280.2024.02.018

中国媒介生物学及控制杂志 ›› 2024, Vol. 35 ›› Issue (2): 232-236.DOI: 10.11853/j.issn.1003.8280.2024.02.018

山西省长治市宠物犬人兽共患病病原菌及巴贝虫感染状况调查

崔永洁¹, 刘益萍², 崔佳², 饶华祥³, 栗冬梅⁴, 于娟²

1. 长治市潞州区动物疫病预防控制中心, 山西长治 046000;
2. 长治医学院基础医学部, 山西长治 046000;
3. 长治医学院公共卫生与预防医学系, 山西长治 046000;
4. 传染病溯源预警与智能决策全国重点实验室, 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 北京 102206

收稿日期:2023-09-13 出版日期:2024-04-20 发布日期:2024-05-09
通讯作者: 于娟,E-mail:yujuan2006008@163.com;栗冬梅,E-mail:lidongmei@icdc.cn
作者简介:崔永洁,男,高级兽医师,主要从事动物疫病防治研究,E-mail:842032053@qq.com
基金资助:
山西省基础研究计划项目(20210302124299);长治医学院学术技术带头人项目(XS202103)

Prevalence of infections with zoonotic bacterial pathogens and Babesia in pet dogs in Changzhi, Shanxi Province, China

CUI Yong-jie¹, LIU Yi-ping², CUI Jia², RAO Hua-xiang³, LI Dong-mei⁴, YU Juan²

1. Luzhou District Center for Animal Disease Control and Prevention, Changzhi, Shanxi 046000, China;
2. Department of Basic Medical Sciences, Changzhi Medical College, Changzhi, Shanxi 046000, China;
3. Department of Public Health and Preventive Medicine, Changzhi Medical College, Changzhi, Shanxi 046000, China;
4. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received:2023-09-13 Online:2024-04-20 Published:2024-05-09

摘要/Abstract

摘要： 目的了解山西省长治市巴尔通体、伯氏疏螺旋体、嗜吞噬细胞无形体、致病性钩端螺旋体(钩体)及巴贝虫等5类人兽共患病病原菌在宠物犬中的感染状况。方法收集宠物犬抗凝血,采用实时荧光定量PCR(qPCR)法进行巴尔通体、伯氏疏螺旋体、嗜吞噬细胞无形体、钩体等细菌性病原体的检测,采用普通PCR法进行巴贝虫的检测,并对阳性PCR产物进行序列测定和分析。组间感染率的差异比较采用Fisher确切概率法。结果共收集98份宠物犬血液标本,结果显示巴尔通体阳性率为4.08 %(4/98),伯氏疏螺旋体阳性率为3.06 %(3/98),嗜吞噬细胞无形体阳性率为7.14 %(7/98),巴贝虫阳性率为3.06 %(3/98),钩体未检出。不同性别、犬龄以及是否接种疫苗的犬只中,各病原体检出率差异均无统计学意义(Fisher确切概率法,P>0.05)。有2只宠物犬存在共感染现象,具体为巴尔通体和嗜吞噬细胞无形体共感染1只,伯氏疏螺旋体和嗜吞噬细胞无形体共感染1只。2份巴贝虫阳性标本测序成功,遗传进化分析显示均为吉氏巴贝虫。结论山西省长治市宠物犬感染多种人兽共患病病原体,具有感染人和其他动物的潜在风险,须做好宠物犬只的管理和疫病防控。

关键词: 宠物犬, 巴尔通体, 伯氏疏螺旋体, 嗜吞噬细胞无形体, 致病性钩端螺旋体, 巴贝虫

Abstract: Objective To investigate the prevalence of five types of infections with zoonotic bacterial pathogens (Bartonella, Borrelia burgdorferi, Anaplasma phagocytophilum, Leptospira, and Babesia) in pet dogs in Changzhi, Shanxi Province, China.Methods Anticoagulant blood samples from pet dogs were collected to detect Babesia (by conventional PCR) and Bartonella, Bor. burgdorferi, A. phagocytophilum, and Leptospira (by quantitative PCR). Positive PCR products were sequenced for analysis. The infection rates between groups were compared using Fisher's exact test.Results A total of 98 blood samples from pet dogs were collected, with 4 of 98 samples (4.08 %) positive for Bartonella, 3 of 98 (3.06 %) positive for Bor. burgdorferi, 7 of 98 (7.14 %) positive for A. phagocytophilum, 3 of 98 (3.06 %) positive for Babesia, and none positive for Leptospira. There was no significant difference in the detection rate of any pathogen between dogs of different sexes, ages, and vaccination status (Fisher's exact test, P>0.05). Two pet dogs had mixed infections, with Bartonella and A. phagocytophilum in one dog and Bor. burgdorferi and A. phagocytophilum in the other one. Two samples positive for Babesia were sequenced successfully, and phylogenetic analysis showed that they were both Bab. gibsoni.Conclusions Multiple zoonotic bacterial pathogens are prevalent in pet dogs in Changzhi, Shanxi Province, which may infect humans and animals. It is necessary to strengthen the management of pet dogs and their epidemic diseases in this area.

Key words: Pet dog, Bartonella, Borrelia burgdorferi, Anaplasma phagocytophilum, Leptospira interrogans, Babesia

中图分类号:

崔永洁, 刘益萍, 崔佳, 饶华祥, 栗冬梅, 于娟. 山西省长治市宠物犬人兽共患病病原菌及巴贝虫感染状况调查[J]. 中国媒介生物学及控制杂志, 2024, 35(2): 232-236.

CUI Yong-jie, LIU Yi-ping, CUI Jia, RAO Hua-xiang, LI Dong-mei, YU Juan. Prevalence of infections with zoonotic bacterial pathogens and Babesia in pet dogs in Changzhi, Shanxi Province, China[J]. Chinese Journal of Vector Biology and Control, 2024, 35(2): 232-236.

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山西省长治市宠物犬人兽共患病病原菌及巴贝虫感染状况调查

Prevalence of infections with zoonotic bacterial pathogens and Babesia in pet dogs in Changzhi, Shanxi Province, China

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