黑龙江省漠河口岸地区2019－2022年鼠类及其携带病原监测结果分析

doi:10.11853/j.issn.1003.8280.2023.06.004

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (6): 728-732.DOI: 10.11853/j.issn.1003.8280.2023.06.004

黑龙江省漠河口岸地区2019－2022年鼠类及其携带病原监测结果分析

于波¹, 刘星媛², 王一平³, 牛磊⁴, 魏子桐², 王强⁵, 梁慧杰⁶

1. 哈尔滨海关技术中心检验部, 黑龙江哈尔滨 151000;
2. 中华人民共和国漠河海关, 黑龙江漠河 165300;
3. 珲春国际旅行卫生保健中心, 吉林珲春 133300;
4. 中华人民共和国抚远海关, 黑龙江抚远 156500;
5. 上海国际旅行卫生保健中心, 上海 200335;
6. 黑龙江国际旅行卫生保健中心检验部, 黑龙江哈尔滨 151000

收稿日期:2023-04-26 出版日期:2023-12-20 发布日期:2023-12-26
通讯作者: 梁慧杰,E-mail：xcbyy03@163.com
作者简介:于波，男，工程师，从事病媒生物监测与控制工作，E-mail：474618777@qq.com
基金资助:
上海市科委项目（22015810600）；海关总署科研项目（2022HK086）

Surveillance and analysis of rodents and pathogens carried by them at Mohe Port in Heilongjiang Province, China, in 2019-2022

YU Bo¹, LIU Xing-yuan², WANG Yi-ping³, NIU Lei⁴, WEI Zi-tong², WANG Qiang⁵, LIANG Hui-jie⁶

1. Department of Medical Laboratory, Harbin Customs Technical Center, Harbin, Heilongjiang 151000, China;
2. Mohe Customs of the People’s Republic of China, Mohe, Heilongjiang 165300, China;
3. Hunchun International Travel Health Care Center, Hunchun, Jilin 133300, China;
4. Fuyuan Customs of the People’s Republic of China, Fuyuan, Heilongjiang 156500, China;
5. Shanghai International Travel Health Care Center, Shanghai 200335, China;
6. Department of Medical Laboratory, Heilongjiang International Travel Health Care Center, Harbin, Heilongjiang 151000, China

Received:2023-04-26 Online:2023-12-20 Published:2023-12-26
Supported by:
Project of Shanghai Municipal Science and Technology Commission (No. 22015810600); Scientific Research Project of General Administration of Customs (No. 2022HK086)

摘要/Abstract

摘要： 目的掌握黑龙江省漠河口岸鼠的种类、分布、季节消长及携带病原体情况,为该口岸深入开展鼠类及相关传染病防治研究提供参考和依据。方法采用夹夜法于2019－2022年每年的5－8月开展鼠密度监测,每月连续监测3 d。应用巢式PCR和普通PCR方法,对采集的鼠类样本开展斑点热群立克次体（SFGR）、鼠疫耶尔森菌、伯氏疏螺旋体等11种鼠源病原体检测。采用χ²检验比较鼠类密度和鼠类携带病原体阳性率差异。结果捕获鼠类1目3科6属7种共208只。黑线姬鼠为该地区优势种,占35.58%；朝鲜姬鼠次之,占16.83%。共检出6种病原体,总阳性率50.48%；其中SFGR阳性率最高（40.38%）,其次为汉坦病毒（11.54%）和伯氏疏螺旋体（3.85%）。病原体复合感染鼠20只,占总阳性数的19.05%,其中3种病原体复合感染鼠1只。结论漠河口岸鼠类携带汉坦病毒、伯氏疏螺旋体和SFGR等多种病原体,应重点加强该地区的鼠类及鼠源疾病的预警监测。

关键词: 口岸, 鼠类, 病原, 监测

Abstract: Objective To investigate the species, distribution, seasonal variation, and pathogens of rodents at Mohe Port in Heilongjiang Province in China, so as to provide a reference for in-depth research on rodent control and the prevention and treatment of related infectious diseases in this port.Methods The night snap trapping method was used to conduct the surveillance of rodent density from May to August in each year from 2019 to 2022, for three consecutive days each month. Nested PCR and ordinary PCR methods were used to detect 11 pathogens in the samples of rodents collected, including spotted fever group Rickettsia (SFGR), Yersinia pestis, and Borrelia burgdorferi. The Chi-square test was used to compare the differences in rodent density and the positive rate of pathogens carried by the rodents.Results A total of 208 rodents of 7 species belonging to 1 order, 3 families, and 6 genera were captured. Apodemus agrarius was the dominant species in this region, accounting for 35.58%, followed by A. peninsulae, accounting for 16.83%. A total of 6 pathogens were detected, with an overall positive rate of 50.48%, among which SFGR had the highest positive rate of 40.38%, followed by Hantavirus (11.54%) and B. burgdorferi (3.85%). There were 20 rodents with co-infection of pathogens, accounting for 19.05% of the total number of rodents with positive pathogens, among which one rodent was infected with 3 pathogens.Conclusion The rodents at Mohe Port carry a variety of pathogens such as Hantavirus, B. burgdorferi, and SFGR, and it is necessary to strengthen the early warning and surveillance of rodents and rodent-borne diseases in this area.

Key words: Port, Rodent, Pathogen, Surveillance

中图分类号:

R184.3
S443

于波, 刘星媛, 王一平, 牛磊, 魏子桐, 王强, 梁慧杰. 黑龙江省漠河口岸地区2019－2022年鼠类及其携带病原监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 728-732.

YU Bo, LIU Xing-yuan, WANG Yi-ping, NIU Lei, WEI Zi-tong, WANG Qiang, LIANG Hui-jie. Surveillance and analysis of rodents and pathogens carried by them at Mohe Port in Heilongjiang Province, China, in 2019-2022[J]. Chinese Journal of Vector Biology and Control, 2023, 34(6): 728-732.

参考文献

[1] Zheng ZM,Jiang ZK,Chen AG. Rodents zoology[M]. 2nd ed. Shanghai:Shanghai Jiao Tong University Press,2012:143-191. （in Chinese）郑智民,姜志宽,陈安国. 啮齿动物学[M]. 2版. 上海:上海交通大学出版社,2012:143-191.
[2] Zhang JW. The identification atlas of medical vectors in Chinese points of entry[M]. Tianjin:Tianjin Science and Technology Publishing House,2015:1-78. （in Chinese）张际文. 中国国境口岸医学媒介生物鉴定图谱[M]. 天津:天津科学技术出版社,2015:1-78.
[3] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China. SN/T 2616-2010 Rapid detection ofYersinia pestisand Hantavirus on rodents at frontier port[S]. Beijing:Standards Press of China,2010. （in Chinese）中华人民共和国国家质量监督检验检疫总局. SN/T 2616―2010 国境口岸鼠携带鼠疫杆菌和汉坦病毒快速检测方法[S]. 北京:中国标准出版社,2010.
[4] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China. SN/T 4616-2016 Detection with PCR forBartonellaat frontier port[S]. Beijing:Standards Press of China,2017. （in Chinese）中华人民共和国国家质量监督检验检疫总局. SN/T 4616―2016 国境口岸巴尔通体PCR检测方法[S]. 北京:中国标准出版社,2017.
[5] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China. SN/T 4611-2016 Nested PCR detecting method for spotted fever group rickettsiae at frontier port[S]. Beijing:Standards Press of China,2017. （in Chinese）中华人民共和国国家质量监督检验检疫总局. SN/T 4611―2016 国境口岸斑点热群立克次体巢式PCR检测方法[S]. 北京:中国标准出版社,2017.
[6] He TJ, Wang Q, He Yet al., Half nested PCR cloning human Bocavirus complete genome from clinical specimen[J]. Int J Lab Med, 2014, 35（1）: 3-5. DOI: 10.3969/j.issn.1673-4130.2014. 01.002. 何涛君,王琼,何英,等. 采用半巢式PCR技术从临床标本中克隆人类博卡病毒的全基因[J]. 国际检验医学杂志,2014,35（1）:3-5. DOI: 10.3969/j.issn.1673-4130.2014.01.002.
[7] Shih CM,Chao LL. Genetic analysis of the outer surface protein C gene of Lyme disease spirochaetes （Borrelia burgdorferi sensu lato） isolated from rodents in Taiwan[J]. J Med Microbiol,2002,51（4）:318-325. DOI:10.1099/0022-1317-51-4-318.
[8] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China. SN∕T 3741.1-2013 Detection for pathogens of rodents at frontier ports-Part 1:Detection for pathogenicLeptospira[S]. Beijing:Standards Press of China,2014. （in Chinese）中华人民共和国国家质量监督检验检疫总局. SN/T 3741.1―2013 国境口岸鼠类携带病原体检测方法第1部分:致病性钩端螺旋体PCR检测方法[S]. 北京:中国标准出版社,2014.
[9] Chu FK. Rapid and sensitive PCR-based detection and differentiation of aetiologic agents of human granulocytotropic and monocytotropic ehrlichiosis[J]. Mol Cell Probes,1998,12（2）:93-99. DOI:10.1006/mcpr.1998.0150.
[10] Lee JH,Park HS,Jong WJ,et al. Identification of the Coxiella sp. detected from Haemaphysalis longicornis ticks in Korea[J]. Microbiol Immunol,2004,48（2）:125-130. DOI:10.1111/j.1348-0421.2004.tb03498.x.
[11] Regnery RL,Spruill CL,Plikaytis BD. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes[J]. J Bacteriol,1991,173（5）:1576-1589. DOI:10.1128/jb.173.5.1576-1589.1991.
[12] Zhang Q,Liu YX,Gao Y,et al. Study on the molecular epidemiology regarding the natural infection of Orientia tsutsugamushi in 4 species of dominant chiggers collected in various seasons from the foci of Shandong province[J]. Chin J Epidemiol,2006,27（7）:600-603. DOI:10.3760/j.issn:0254-6450.2006.07.012.（in Chinese）张倩,刘运喜,高媛,等. 山东地区不同季节优势恙螨自然感染恙虫病东方体的分子流行病学调查[J]. 中华流行病学杂志,2006,27（7）:600-603. DOI:10.3760/j.issn:0254-6450. 2006.07.012.
[13] Ju WD,Cheng C,Fu WM,et al. Investigation of spotted fever group Rickettsia in Heilongjiang Port[J]. Acta Parasitol Med Entomol Sin,2015,22（1）:37-43. DOI:10.3969/j.issn.1005-0507.2015.01.007.（in Chinese）鞠文东,程成,付维明,等. 黑龙江口岸斑点热群立克次体调查研究[J]. 寄生虫与医学昆虫学报,2015,22（1）:37-43. DOI:10.3969/j.issn.1005-0507.2015.01.007.
[14] Liang HJ,Wang DW,Wang YM,et al. Analysis of special surveillance on rodents at border ports in Heilongjiang province from 2019 to 2020[J]. China Port Sci Technol,2021,3（10）:23-29. DOI:10.3969/j.issn.1002-4689.2021.10.004.（in Chinese）梁慧杰,汪东伟,王玉梅,等. 黑龙江省边境口岸2019－2020年鼠形动物专项监测分析[J]. 中国口岸科学技术,2021,3（10）:23-29. DOI:10.3969/j.issn.1002-4689.2021.10.004.
[15] Zhan L,Cao WC,Chu CY,et al. Tick-borne agents in rodents,China,2004-2006[J]. Emerg Infect Dis,2009,15（12）:1904-1908. DOI:10.3201/eid1512.081141.
[16] Zuo SY,Tang K,Zheng YC,et al. DNA detection and sequence analysis of spotted fever group rickettsiae in rodents from Heilongjiang forest region[J]. J Cent South Univ:Med Sci,2013,38（5）:443-447. DOI:10.3969/j.issn.1672-7347.2013. 05.002.（in Chinese）左双燕,唐琨,郑元春,等. 黑龙江林区野鼠中斑点热群立克次体的核酸检测与序列分析[J]. 中南大学学报:医学版,2013,38（5）:443-447. DOI:10.3969/j.issn.1672-7347.2013.05.002.
[17] Zhang JZ,Fan MY. Research achievements of spotted fever in China[J]. Chin J Epidemiol,1999,20（2）:71-73. DOI:10.3760/j.issn:0254-6450.1999.02.002.（in Chinese）张健之,范明远. 中国斑点热的研究成就[J]. 中华流行病学杂志,1999,20（2）:71-73. DOI:10.3760/j.issn:0254-6450.1999. 02.002.
[18] Cao XM,Hu KX,Wang J,et al. A survey of rodent and rodent-borne ectozoa in ports between China and Russia[J]. Acta Parasitol Med Entomol Sin,2011,18（4）:230-235. DOI:10.3969/j.issn.1005-0507.2011.04.007.（in Chinese）曹晓梅,胡孔新,王静,等. 中俄双边口岸鼠类及体表寄生虫调查[J]. 寄生虫与医学昆虫学报,2011,18（4）:230-235. DOI:10.3969/j.issn.1005-0507.2011.04.007.
[19] Zhao YQ,Cheng P,Gong MQ,et al. Summary of mouse-spread diseases and measures to control them[J]. J Pathog Biol,2010,5（5）:378-380. DOI:10.13350/j.cjpb.2010.05.005.（in Chinese）赵玉强,程鹏,公茂庆,等. 鼠传播疾病及鼠类的防制概述[J]. 中国病原生物学杂志,2010,5（5）:378-380. DOI:10.13350/j.cjpb.2010.05.005.
[20] Gutiérrez R,Morick D,Cohen C,et al. The effect of ecological and temporal factors on the composition of Bartonella infection in rodents and their fleas[J]. ISME J,2014,8（8）:1598-1608. DOI:10.1038/ismej.2014.22.
[21] Bai Y,Kosoy MY,Maupin GO,et al. Discovery of Bartonella species in rodents in Yunnan[J]. Chin J Zoonoses,2002,18（3）:5-9. DOI:10.3969/j.issn.1002-2694.2002.03.001.（in Chinese）白瑛,Kosoy MY,Maupin GO,等. 首次证实巴尔通体在我国云南鼠群中流行[J]. 中国人兽共患病杂志,2002,18（3）:5-9. DOI:10.3969/j.issn.1002-2694.2002.03.001.
[22] Li DM,Xu AL,Song XP,et al. Bartonella distribution in naturally infected rodent tissues[J]. Chin J Vector Biol Control,2021,32（2）:181-187. DOI:10.11853/j.issn.1003.8280.2021. 02.012.（in Chinese）栗冬梅,徐爱玲,宋秀平,等. 巴尔通体在自然感染的啮齿动物组织中的分布[J]. 中国媒介生物学及控制杂志,2021,32（2）:181-187. DOI:10.11853/j.issn.1003.8280.2021.02.012.
[23] Yu D,Yin JX. Progress in research of natural infection status of Bartonella in rodents[J]. Dis Surveill,2018,33（1）:15-19. DOI:10.3784/j.issn.1003-9961.2018.01.005.（in Chinese）俞丹,尹家祥. 鼠形动物自然感染巴尔通体的研究进展[J]. 疾病监测,2018,33（1）:15-19. DOI:10.3784/j.issn.1003-9961. 2018.01.005.

黑龙江省漠河口岸地区2019－2022年鼠类及其携带病原监测结果分析

Surveillance and analysis of rodents and pathogens carried by them at Mohe Port in Heilongjiang Province, China, in 2019-2022

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	金彬彬, 周倩倩, 韦凌娅, 段恬筱, 王英红, 许永杰, 金慧. 杭州市2017－2021年蝇类生态学监测结果及其与气象因素的相关性分析[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 733-738.
[2]	王莉燕, 周慧, 陈忠兵, 方春福, 曹国平, 朱建英, 张英, 龚震宇. 世界灌溉工程遗产浙江省龙游县姜席堰2019－2021年病媒生物监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 744-748.
[3]	侯银续, 张燕婕, 王文君, 仰凤桃, 刘小波, 孟凤霞, 徐庆华. 安徽省合肥市2021年登革热媒介白纹伊蚊对常用杀虫剂的抗药性水平[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 749-753.
[4]	肖珊, 陈建勇, 林斌, 龙建勋, 彭莱, 朱彩英. 自回归积分移动平均模型在长沙市蝇密度预测中的应用[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 788-793.
[5]	孙钦同, 刘言, 韩英男, 胡巨凤, 王学军, 刘文杰, 曹馨月, 赖世宏, 何倩, 景晓. 气象因素对济南市城区蚊密度影响及滞后效应分析[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 799-803,813.
[6]	刘道华, 金郁, 李启扬, 杨荣笙, 汪天平, 操治国. 安徽省休宁县2019－2021年并殖吸虫中间宿主监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 617-621.
[7]	赵怡双, 菅越, 尤晓杰, 王敏雅, 陈财荣. 浙江省台州市2017－2022年媒介蚊虫生态学监测及病毒携带状况调查[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 622-627.
[8]	肖汉森, 何亚明, 涂涛田, 张应, 王政, 季恒青. 重庆市2021－2022年鼠传病原体监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 628-632.
[9]	刘福生, 薛培苗, 黄喜平, 王宝忠, 郑梅. 内蒙古乌海市2013－2022年鼠疫监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 633-636.
[10]	郭承德, 秦萌, 韩立亮, 付和平, 刘宝玉, 苏元红, 肖彩虹, 阿拉塔, 蔺永生, 段锐, 王登云, 乔靖然. 内蒙古西部农区基于智能物联网的鼠害监测系统待机时间及影响因素分析[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 686-690.
[11]	陈红, 周洲, 刘洪霞. 基于网格化的诱蚊诱卵器法与人诱停落法监测白纹伊蚊对照研究[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 451-456.
[12]	戈斌, 张海兵, 刘洪霞, 周瑛, 刘清, 易可华, 黄涛. 上海市奉贤区2011－2021年蝇类生态学监测分析[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 462-466.
[13]	刘俊, 林晨, 元永艇, 于思雨, 刘婧怡, 李慧慧, 冯磊, 顾盈培, 刘汉昭. 上海市浦东新区餐饮场所蜚蠊侵害及携带肠道病原体分析[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 467-471.
[14]	周良才, 周仲瑾, 王莹, 吴太平, 吴丽群, 汤伟峰. 第七届世界军人运动会病媒生物监测与控制效果评估[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 485-490.
[15]	金彬彬, 韦凌娅, 金慧, 曹阳, 王英红, 孔庆鑫. 杭州市2017－2021年登革热媒介白纹伊蚊生态学监测结果分析[J]. 中国媒介生物学及控制杂志, 2023, 34(4): 491-496.