实验室条件下长角血蜱胚胎发育过程

doi:10.11853/j.issn.1003.8280.2023.06.003

摘要/Abstract

摘要： 目的在实验室条件下观察两性和孤雌生殖方式长角血蜱的胚胎发育过程,为该种群数量动态预测和控制提供依据。方法在温度为（26±1） ℃,相对湿度为（75±5）%,光周期（L∶D）为16 h∶8 h条件下,应用胚胎固定和4',6-二脒基-2-苯基吲哚（DAPI）染色技术对长角血蜱胚胎发育过程进行观察描述。组间差异比较采用独立样本t检验。结果孤雌生殖长角血蜱胚胎发育历期[（24.6±1.7） d]显著长于两性生殖长角血蜱[（22.5±3.5） d]（t=2.699,P=0.010）。两种生殖种群胚胎发育过程无明显差异。胚胎发育过程大致分为：发育1 d,初期胚胎能够识别到细胞核；发育4 d,细胞数量显著增加,胚盘出现；发育6 d,胚胎体节和腹侧沟出现；发育7 d,能够明显区分出头部、胸部、生长区,3对附肢（1L～3L）原基清晰,第4附肢（4L）明显短于前3对附肢,发育12 d,腹侧沟关闭,第4附肢开始退化,另外3对附肢变大,胚胎收缩到前腹区,背侧开始闭合；发育16 d,背侧闭合继续发生,头部的螯肢定位发生改变；发育20 d,背侧闭合完成,胚胎开始孵化。结论明确了长角血蜱胚胎在实验室条件下的发育过程,为该蜱数量动态的预测和控制提供了依据。

关键词: 长角血蜱, 胚胎发育, 胚胎固定, 4', 6-二脒基-2-苯基吲哚染色, 形态变化

Abstract: Objective To study the embryonic development of bisexual and parthenogenetic Haemaphysalis longicornis, so as to provide a basis for dynamic prediction and control of this species.Methods The embryonic development process of H. longicornis was recorded after embryo fixation and 4',6-diamidino-2-phenylindole staining under laboratory conditions (temperature, [26±1] ℃; relative humidity, [75±5]%; photoperiod, 16L∶8D). The independent samples t-test was used for comparison of differences between groups.Results The embryonic developmental period of parthenogenetic ticks ([24.6±1.7] d) was significantly longer than that of bisexual ticks ([22.5±3.5] d) (t=2.699, P=0.010). There was no significant difference in embryonic development between the two reproductive populations. The embryonic development process was generally as following: on day 1, the nucleus could be observed in the early embryo; on day 4, the number of cells increased significantly, and the blastoderm appeared; on day 6, the embryonic body segments and ventral furrow appeared; on day 7, the head, thorax, and growth zone could be clearly distinguished, the anlagen of the three pairs of legs (1L-3L) became clear, and the fourth leg pair (4L) was significantly shorter than the first three leg pairs; on day 12, the ventral furrow closed, the fourth pair of legs began to degenerate, the other three pairs of legs became larger, the embryo contracted to the anterior ventral region, and the dorsal region began to close; on day 16, the dorsal region continued to close, and the position of the chelicerae on the head changed; on day 20, dorsal closure was completed, and the embryo began to hatch.Conclusion This study presented the embryonic developmental process of H. longicornis under laboratory conditions, providing a reference for dynamic prediction and control of H. longicornis populations.

Key words: Haemaphysalis longicornis, Embryonic development, Embryo fixation, 4',6-Diamidino-2-phenylindole staining, Morphological change

中图分类号:

陈凯丽, 杨晨, 张雨凡, 李思思, 刘敬泽, 张艳凯. 实验室条件下长角血蜱胚胎发育过程[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 723-727.

CHEN Kai-li, YANG Chen, ZHANG Yu-fan, LI Si-si, LIU Jing-ze, ZHANG Yan-kai. The embryonic development process of Haemaphysalis longicornis under laboratory conditions[J]. Chinese Journal of Vector Biology and Control, 2023, 34(6): 723-727.

参考文献

[1] Zhao JW,Wang HY,Wang Y. Regional distribution profiles of tick-borne pathogens in China[J]. Chin J Vector Biol Control,2012,23（5）:445-448. （in Chinese）赵俊伟,王环宇,王英. 中国蜱传病原体分布研究概况[J]. 中国媒介生物学及控制杂志,2012,23（5）:445-448.
[2] Anderson JF,Magnarelli LA. Biology of ticks[J]. Infect Dis Clin N Am,2008,22（2）:195-215. DOI:10.1016/j.idc.2007.12.006.
[3] Nava S,Martínez JG,Arreguez GA,et al. Ticks （Acari:Argasidae,Ixodidae） from Middle and pre-Hispanic Late Holocene associated with human activities in northwestern Argentina[J]. Ticks Tick-Borne Dis,2013,4（1/2）:167-169. DOI:10.1016/j.ttbdis.2012.10.037.
[4] Rosà R,Pugliese A. Effects of tick population dynamics and host densities on the persistence of tick-borne infections[J]. Math Biosci,2007,208（1）:216-240. DOI:10.1016/j.mbs.2006. 10.002.
[5] Wu HX,Liu XB,Yue YJ,et al. National surveillance report on ticks in China,2019[J]. Chin J Vector Biol Control,2020,31（4）:417-422. DOI:10.11853/j.issn.1003.8280.2020.04.007.（in Chinese）吴海霞,刘小波,岳玉娟,等. 2019年全国蜱类监测报告[J]. 中国媒介生物学及控制杂志,2020,31（4）:417-422. DOI:10.11853/j.issn.1003.8280.2020.04.007.
[6] Liu XF,Zhou ZB,Wang W,et al. Influence of weather factors on questing activity of Haemaphysalis longicornis[J]. Sci Silv Sin,2020,56（6）:68-75. DOI:10.11707/j.1001-7488.20200607.（in Chinese）柳絮飞,周在豹,王巍,等. 气象因子对长角血蜱寻觅活动的影响[J]. 林业科学,2020,56（6）:68-75. DOI:10.11707/j.1001-7488.20200607.
[7] Dyczko D,Kiewra D,Kolanek A,et al. The influence of local environmental factors in southwestern Poland on the abundance of Ixodes ricinus and prevalence of infection with Borrelia burgdorferi s.l. and B. miyamotoi[J]. Parasitol Res,2022,121（6）:1575-1585. DOI:10.1007/s00436-022-07493-9.
[8] Stafford III KC,Williams SC,Molaei G. Integrated pest management in controlling ticks and tick-associated diseases[J]. J Integr Pest Manag,2017,8（1）:28. DOI:10.1093/jipm/pmx018.
[9] Li ZX,Liu GY,Tian ZC,et al. Effects of different feeding mode and temperature on biological characteristics of Haemaphysalis longicornis[J]. Prog Vet Med,2010,31（5）:26-31. DOI:10. 16437/j.cnki.1007-5038.2010.05.016.（in Chinese）李知新,刘光远,田占成,等. 不同饲喂方式和温度对长角血蜱甘肃株生物学特性的影响[J]. 动物医学进展,2010,31（5）,26-31. DOI:10.16437/j.cnki.1007-5038.2010.05.016.
[10] Yano Y,Shiraishi S,Uchida TA. Effects of temperature on development and growth in the tick,Haemaphysalis longicornis[J]. Exp Appl Acarol,1987,3（1）:73-78. DOI:10.1007/BF01200415.
[11] Magdaş C,Magdaş VA,Mihalca AD,et al. Laboratory development of Dermacentor marginatus ticks （Acari:Ixodidae） at two temperatures[J]. Exp Appl Acarol,2015,67（2）:309-315. DOI:10.1007/s10493-015-9942-z.
[12] Zhao L,Li J,Cui XM,et al. Distribution of Haemaphysalis longicornis and associated pathogens:Analysis of pooled data from a China field survey and global published data[J]. Lancet Planet Health,2020,4（8）:e320-e329. DOI:10.1016/S2542-5196（20）30145-5.
[13] Chen Z,Yang XJ,Bu FJ,et al. Morphological,biological and molecular characteristics of bisexual and parthenogenetic Haemaphysalis longicornis[J]. Vet Parasitol,2012,189（2/4）:344-352. DOI:10.1016/j.vetpar.2012.04.021.
[14] Santos VT,Ribeiro L,Fraga A,et al. The embryogenesis of the tick Rhipicephalus （Boophilus） microplus:The establishment of a new chelicerate model system[J]. Genesis,2013,51（12）:803-818. DOI:10.1002/dvg.22717.
[15] Zheng HY,Yu ZJ,Chen Z,et al. Development and biological characteristics of Haemaphysalis longicornis （Acari:Ixodidae） under field conditions[J]. Exp Appl Acarol,2011,53（4）:377-388. DOI:10.1007/s10493-010-9415-3.
[16] Zhang TT,Qiu ZX,Li Y,et al. The mRNA expression and enzymatic activity of three enzymes during embryonic development of the hard tick Haemaphysalis longicornis[J]. Parasit Vectors,2019,12（1）:96. DOI:10.1186/s13071-019-3360-8.
[17] Wang D,Li MM,Ma JY,et al. Effects of temperature on cathepsin B,cathepsin D and acid phosphatase during embryo development of the hard tick Haemaphysalis longicornis[J]. Exp Appl Acarol,2023,89（1）:105-115. DOI:10.1007/s10493-022-00774-z.
[18] Liu QY. Impact of climate change on vector-borne diseases and related response strategies in China:Major research findings and recommendations for future research[J]. Chin J Vector Biol Control,2021,32（1）:1-11. DOI:10.11853/j.issn.1003.8280. 2021.01.001.（in Chinese）刘起勇. 气候变化对中国媒介生物传染病的影响及应对:重大研究发现及未来研究建议[J]. 中国媒介生物学及控制杂志,2021,32（1）:1-11. DOI:10.11853/j.issn.1003.8280.2021. 01.001.
[19] Friesen KJ,Dixon M,Lysyk TJ. Embryo development and morphology of the rocky mountain wood tick （Acari:Ixodidae）[J]. J Med Entomol,2016,53（2）:279-289. DOI:10.1093/jme/tjv193.
[20] da Silva JN,Simas DLR,Soares AR,et al. Glucose metabolomic profile during embryogenesis in the tick Rhipicephalus microplus[J]. Metabolomics,2021,17（9）:79. DOI:10.1007/s11306-021-01830-2.
[21] Zhang RL,Liu WJ,Zhang KX,et al. Developmental transcriptomics throughout the embryonic developmental process of Rhipicephalus turanicus reveals stage-specific gene expression profiles[J]. Parasit Vectors,2022,15（1）:89. DOI:10.1186/s13071-022-05214-w.
[22] Seixas A,Oliveira P,Termignoni C,et al. Rhipicephalus （Boophilus） microplus embryo proteins as target for tick vaccine[J]. Vet Immunol Immunopathol,2012,148（1/2）:149-156. DOI:10.1016/j.vetimm.2011.05.011.
[23] Nuss A,Sharma A,Gulia-Nuss M. Genetic manipulation of ticks:A paradigm shift in tick and tick-borne diseases research[J]. Front Cell Infect Microbiol,2021,11:678037. DOI:10.3389/fcimb.2021.678037.