实验室品系家蝇对常用杀虫剂敏感基线的建立

doi:10.11853/j.issn.1003.8280.2023.06.002

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (6): 719-722.DOI: 10.11853/j.issn.1003.8280.2023.06.002

实验室品系家蝇对常用杀虫剂敏感基线的建立

张亚军¹, 赵春春¹, 冯星明², 王佳琳¹, 伦辛畅¹, 刘起勇¹, 孟凤霞¹

1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病溯源预警与智能决策全国重点实验室, WHO媒介生物监测与管理合作中心, 北京 102206;
2. 云南省地方病防治所, 云南大理 671000

收稿日期:2023-06-29 出版日期:2023-12-20 发布日期:2023-12-26
通讯作者: 孟凤霞,E-mail：mengfengxia@icdc.cn
作者简介:张亚军，女，在读硕士，主要从事媒介生物抗药性研究，E-mail：zyj1483@163.com
基金资助:
媒介生物监测与控制项目（102393220020020000012）；国家科技重大专项（2018ZX10101002-002）

Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides

ZHANG Ya-jun¹, ZHAO Chun-chun¹, FENG Xing-ming², WANG Jia-lin¹, LUN Xin-chang¹, LIU Qi-yong¹, MENG Feng-xia¹

1. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, WHO Collaborating Centre for Vector Surveillance and Management, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China;
2. Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan 671000, China

Received:2023-06-29 Online:2023-12-20 Published:2023-12-26
Supported by:
Vector Surveillance and Control Project (No. 102393220020020000012); National Science and Technology Major Project of China (No. 2018ZX10101002-002)

摘要/Abstract

摘要： 目的该研究旨在建立家蝇对6种常用杀虫剂的敏感基线,为我国家蝇抗药性系统监测和防控提供依据。方法利用点滴法测定本实验室常年饲养的家蝇敏感品系对4大类共6种杀虫剂（溴氰菊酯、高效氯氰菊酯、氯菊酯、敌敌畏、残杀威、呋虫胺）的敏感性,同时收集历史文献中的数据,以半数致死剂量（LD₅₀）的95%置信区间（95%CI）是否重叠来判断敏感性差异有无统计学意义,基于以上方法建立家蝇对这6种杀虫剂的敏感基线。结果供试的6种杀虫剂对家蝇的敏感基线依次为：溴氰菊酯（LD₅₀=0.002 236 μg/♀,y=13.76+5.19x）、高效氯氰菊酯（LD₅₀=0.003 692 μg/♀,y=9.34+3.84x）、氯菊酯（LD₅₀=0.007 152 μg/♀,y=10.03+4.67x）、敌敌畏（LD₅₀=0.074 20 μg/♀,y=5.02+4.44x）、残杀威（LD₅₀=0.415 9 μg/♀,y=0.93+2.43x）、呋虫胺（LD₅₀=0.103 6 μg/♀,y=6.31+6.41x）。结论测定了实验室敏感品系家蝇对6种常用杀虫剂的敏感水平,建立了敏感基线,为我国家蝇抗药性监测和抗性水平评估提供了参考依据。

关键词: 家蝇, 点滴法, 杀虫剂, 敏感基线

Abstract: Objective To establish the sensitive baseline of houseflies to six commonly used insecticides, so as to provide a basis for the monitoring and control of the insecticide resistance of houseflies.Methods The topical application method was used to test the sensitivity of laboratory susceptible housefly strains to six insecticides (deltamethrin, beta-cypermethrin, permethrin, dichlorvos, propoxur, and dinotefuran) of four categories. Data from previous literature were collected for comparison. The statistical significance of sensitivity differences was determined according to whether the 95% confidence interval of the median lethal dose (LD₅₀) overlapped. Based on the above steps, the baseline sensitivity of houseflies to the six insecticides was established.Results The baseline sensitivity of the six test insecticides against houseflies were as follows: deltamethrin (LD₅₀=0.002 236 μg/♀, y=13.76+5.19x), beta-cypermethrin (LD₅₀=0.003 692 μg/♀, y=9.34+3.84x), permethrin (LD₅₀=0.007 152 μg/♀, y=10.03+4.67x), dichlorvos (LD₅₀=0.074 20 μg/♀, y=5.02+4.44x), propoxur (LD₅₀=0.415 9 μg/♀, y=0.93+2.43x), and dinotefuran (LD₅₀=0.103 6 μg/♀, y=6.31+6.41x).Conclusion This study established the baseline sensitivity of houseflies through testing the sensitivity of laboratory susceptible strains to six commonly used insecticides, providing a reference for monitoring and assessing the resistance level of houseflies in China.

Key words: Housefly, Topical application, Insecticide, Baseline sensitivity

中图分类号:

张亚军, 赵春春, 冯星明, 王佳琳, 伦辛畅, 刘起勇, 孟凤霞. 实验室品系家蝇对常用杀虫剂敏感基线的建立[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 719-722.

ZHANG Ya-jun, ZHAO Chun-chun, FENG Xing-ming, WANG Jia-lin, LUN Xin-chang, LIU Qi-yong, MENG Feng-xia. Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides[J]. Chinese Journal of Vector Biology and Control, 2023, 34(6): 719-722.

参考文献

[1] Scott JG. Evolution of resistance to pyrethroid insecticides in Musca domestica[J]. Pest Manag Sci,2017,73（4）:716-722. DOI:10.1002/ps.4328.
[2] Hou YX,Liu ZW,Zhang YJ,et al. Resistance of Musca domestica to six insecticides commonly used in Hefei,China,2021[J]. Chin J Vector Biol Control,2023,34（1）:105-108. DOI:10.11853/j.issn.1003.8280.2023.01.019.（in Chinese）侯银续,刘志伟,张燕婕,等. 2021年合肥市家蝇对6种常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2023,34（1）:105-108. DOI:10.11853/j.issn.1003.8280.2023. 01.019.
[3] Li HL,Li YZ,Wu HY,et al. Introduction on roles of houseflies in transmission of animal infectious diseases[J]. Prog Vet Med,2017,38（2）:107-110. （in Chinese）李好磊,李叶珍,吴浩阳,等. 家蝇在动物疫病传播中的作用研究概况[J]. 动物医学进展,2017,38（2）:107-110.
[4] Scott JG,Leichter CA,Rinkevihc FD,et al. Insecticide resistance in house flies from the United States:Resistance levels and frequency of pyrethroid resistance alleles[J]. Pestic Biochem Physiol,2013,107（3）:377-384. DOI:10.1016/j.pestbp.2013. 10.006.
[5] Wu YY,Liu QM,Wang JN,et al. Resistance of Musca domestica to commonly used insecticides in Zhejiang province,China,2021[J]. Chin J Vector Biol Control,2023,34（1）:26-30. DOI:10.11853/j.issn.1003.8280.2023.01.005.（in Chinese）吴瑜燕,刘钦梅,王金娜,等. 2021年浙江省家蝇对常见卫生杀虫剂的抗药性研究[J]. 中国媒介生物学及控制杂志,2023,34（1）:26-30. DOI:10.11853/j.issn.1003.8280.2023.01.005.
[6] Liu QY, Liu XB, Chang N, et al. Advances and achievements in the surveillance and control of vectors and vector-borne diseases in China,2012-2021[J]. Chin J Vector Biol Control,2022,33（5）:613-621,654. DOI:10.11853/j.issn.1003.8280.2022.05. 001. （in Chinese）刘起勇,刘小波,常楠,等. 2012－2021年我国媒介生物及相关传染病监测控制进展及成效[J]. 中国媒介生物学及控制杂志,2022,33（5）:613-621,654. DOI:10.11853/j.issn.1003.8280. 2022.05.001.
[7] Wang HH,Lyu SL,Zhao R,et al. Establishment of the relative susceptible baselines of Spodoptera frugiperda （Lepidoptera:Noctuidae） larvae to commonly used insecticides[J]. Acta Entomol Sin,2021,64（12）:1427-1432. DOI:10.16380/j.kcxb. 2021.12.008.（in Chinese）王欢欢,吕圣兰,赵瑞,等. 草地贪夜蛾幼虫对常用杀虫剂相对敏感基线的建立[J]. 昆虫学报,2021,64（12）:1427-1432. DOI:10.16380/j.kcxb.2021.12.008.
[8] Zhai GR. Determination of resistance in houseflies and breeding of resistance[J]. Insect Knowl,1982（5）:47-48,42. （in Chinese）翟桂荣. 家蝇抗药性的测定及抗性的培育[J]. 昆虫知识,1982（5）:47-48,42.
[9] Zhang Y,Meng FX,Liu QY,et al. The susceptibility of female adult house fly （Musca domestica） at different eclosion days to deltamethrin[J]. Chin J Vector Biol Control,2005,16（2）:95-97. DOI:10.3969/j.issn.1003-4692.2005.02.005.（in Chinese）张莹,孟凤霞,刘起勇,等. 家蝇雌成虫对溴氰菊酯的敏感性研究[J]. 中国媒介生物学及控制杂志,2005,16（2）:95-97. DOI:10.3969/j.issn.1003-4692.2005.02.005.
[10] General Administration of Quality Supervision,Inspection and Quarantine of the People’s Republic of China,Standardization Administration of the People’s Republic of China. GB/T 26350-2010 Test methods of fly resistance to insecticides-The bioassay methods forMusca domestica[S]. Beijing:China Standard Press,2011. （in Chinese）中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会. GB/T 26350－2010 蝇类抗药性检测方法家蝇生物测定法[S]. 北京:中国标准出版社,2011.
[11] Liu Y,Zhang SH,Liang ZN,et al. Study on insecticide resistance difference of Aedes albopictus between adjacent sites[J]. Chin J Vector Biol Control,2020,31（4）:490-493. DOI:10.11853/j.issn.1003.8280.2020.04.024.（in Chinese）刘阳,张韶华,梁焯南,等. 相邻场所白纹伊蚊抗药性差异研究[J]. 中国媒介生物学及控制杂志,2020,31（4）:490-493. DOI:10.11853/j.issn.1003.8280.2020.04.024.
[12] Chen DP, Zhou YZ, Qian WP,et al. Study on the insecticide resistance of Musca domestica vicina and control strategies in Sichuan province[J]. Chin J Hyg Insect Equip,2011,17（3）:184-187. （in Chinese）陈东平,周忆昭,钱薇萍,等. 四川省5.12地震灾区家蝇抗药性水平研究[J]. 中华卫生杀虫药械,2011,17（3）:184-187.
[13] Qi YP,Fu XF,Zha YW,et al. Study on resistance of main vectors to commonly used insecticides in Jiaxing,China[J]. Chin J Vector Biol Control,2019,30（3）:337-340. DOI:10.11853/j.issn.1003.8280.2019.03.026.（in Chinese）亓云鹏,富小飞,查亦薇,等. 浙江省嘉兴市主要病媒生物对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2019,30（3）:337-340. DOI:10.11853/j.issn.1003.8280. 2019.03.026.
[14] Liu KH,Lyu GB,Jin ZJ,et al. Resistance of Musca domestica to 5 pesticides in Handan city[J]. J Med Pest Control,2015,31（12）:1381-1382,1385. DOI:10.7629/yxdwfz201512028.（in Chinese）刘凯华,吕广波,靳增军,等. 邯郸市家蝇对5种杀虫剂的抗药性研究[J]. 医学动物防制,2015,31（12）:1381-1382,1385. DOI:10.7629/yxdwfz201512028.
[15] Lyu W,Huo LX,Sun YX. Investigation of resistance of Musca domestica to insecticides in the four areas of Shaanxi province [J]. Chin J Vector Biol Control,2012,23（4）:314-316. （in Chinese）吕文,霍丽霞,孙养信. 陕西省不同地区家蝇抗药性调查[J]. 中国媒介生物学及控制杂志,2012,23（4）:314-316.
[16] Lu YH,Yang T,Gao XW. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi （Linnaeus） and Sitobion avenae （Fabricius）（Homoptera:Aphididae） by the method of residual film in glass tube [J]. Acta Entomol Sin,2009,52（1）:52-58. DOI:10.3321/j.issn:0454-6296.2009.01.008.（in Chinese）鲁艳辉,杨婷,高希武. 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立[J]. 昆虫学报,2009,52（1）:52-58. DOI:10.3321/j.issn:0454-6296.2009.01.008.
[17] Khan HAA. Characterization of permethrin resistance in a Musca domestica strain:Resistance development,cross-resistance potential and realized heritability [J]. Pest Manag Sci,2019,75（11）:2969-2974. DOI:10.1002/ps.5409.
[18] Shan C,Zhang Y,Ma Z,et al. Inheritance of propoxur resistance in a near-isogenic line of Musca domestica （Diptera:Muscidae） [J]. J Econ Entomol, 2016,109（2）:873-878. DOI:10.1093/jee/tow001.
[19] Liu ZQ,Yuan LF,Liao XJ,et al. Research progress of neonicotinoid insecticide dinotefuran[J]. Mod Agrochem,2021,20（1）:7-12. DOI:10.3969/j.issn.1671-5284.2021.01.002.（in Chinese）刘子琪,袁龙飞,廖先骏,等. 新烟碱类杀虫剂呋虫胺的研究进展[J]. 现代农药,2021,20（1）:7-12. DOI:10.3969/j.issn.1671-5284.2021.01.002.

实验室品系家蝇对常用杀虫剂敏感基线的建立

Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	侯银续, 张燕婕, 王文君, 仰凤桃, 刘小波, 孟凤霞, 徐庆华. 安徽省合肥市2021年登革热媒介白纹伊蚊对常用杀虫剂的抗药性水平[J]. 中国媒介生物学及控制杂志, 2023, 34(6): 749-753.
[2]	侯银续, 王斐, 安洲, 仝飞, 芮振宇, 台德运, 任东升, 孟凤霞, 吴磊, 徐庆华. 安徽省芜湖市2022年德国小蠊对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 612-616.
[3]	宋丙栋, 张艳, 闵玥, 陈学敏, 陈航, 孙楠, 陆墨原, 王冲, 张守刚. 南京市2022年蝇类生态学监测及家蝇对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 637-641.
[4]	武佳慧, 彭荟, 公茂庆. 媒介蚊虫杀虫剂抗性对其媒介能力影响的研究进展[J]. 中国媒介生物学及控制杂志, 2023, 34(5): 708-712.
[5]	赵奇, 樊金星, 张叶, 岳思宁, 刘吉起. 河南省家蝇种群对拟除虫菊酯类杀虫剂敏感性测定和击倒抗性基因突变研究[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 308-313.
[6]	华栋栋, 王磊, 肖迪, 李文玉, 周欣欣, 伦辛畅, 母群征, 刘起勇, 马伟, 孟凤霞. 白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 196-203.
[7]	兰学梅, 魏春, 朱进, 李俊明, 罗春海, 杨明东, 容艺函宇, 姜进勇. 中国-老挝登革热重点地区埃及伊蚊孳生习性及杀虫剂抗性水平比较研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 238-243.
[8]	吴丽群, 陈晓敏, 包继永, 周良才. 武汉市2019－2021年白纹伊蚊对常见卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2023, 34(1): 101-104.
[9]	侯银续, 刘志伟, 张燕婕, 仰凤桃, 王文君, 孟凤霞, 黄发源. 2021年合肥市家蝇对6种常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2023, 34(1): 105-108.
[10]	吴瑜燕, 刘钦梅, 王金娜, 李天奇, 罗明宇, 龚震宇. 2021年浙江省家蝇对常见卫生杀虫剂的抗药性研究[J]. 中国媒介生物学及控制杂志, 2023, 34(1): 26-30.
[11]	王丹, 陈丹丹, 田冬冬, 徐秀平, 周敬祝, 师伟芳, 梁文琴. 贵州省铜仁市白纹伊蚊抗药性及代谢酶活性监测研究[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 787-792.
[12]	江汉鹏, 饶志云, 凌春燕, 杨兴华, 谢军. 江西省抚州市2021年白纹伊蚊生态学、抗药性监测及卫生杀虫剂使用状况调查[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 828-833.
[13]	吴群, 孙定炜, 李善干, 曾雪霞, 曾林海, 王绍萍, 吴德雷, 魏家佳, 黎灵刚, 陈成荣, 刘莹, 陈言. 海南省三亚市中华按蚊抗药性及抗性相关基因突变调查[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 666-671.
[14]	陈秀洁, 杨小军, 开文龙, 师灿南, 王义冠, 赵春春, 伦辛畅, 孟凤霞. 杀虫剂稀释倍数和雾滴粒径对白纹伊蚊控制效果的影响[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 748-752.
[15]	韦凌娅, 孔庆鑫, 曹阳, 王慧敏, 沈林海, 王英红. 杭州市家蝇高效氯氰菊酯抗性品系的建立[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 458-461.