浙江省舟山群岛部分地区2021年汉坦病毒宿主调查与病原学分析

doi:10.11853/j.issn.1003.8280.2023.03.008

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (3): 326-330.DOI: 10.11853/j.issn.1003.8280.2023.03.008

浙江省舟山群岛部分地区2021年汉坦病毒宿主调查与病原学分析

柴能明¹, 谭启龙², 舒纪为², 叶凌², 张彤杰², 李世波³, 张森⁴

1. 岱山县第一人民医院感染科, 浙江舟山 316200;
2. 岱山县疾病预防控制中心传染病预防与控制科, 浙江舟山 316200;
3. 温州医科大学附属舟山医院感染科, 浙江舟山 316000;
4. 舟山市定海区疾病预防控制中心, 浙江舟山 316000

收稿日期:2023-01-12 出版日期:2023-06-20 发布日期:2023-06-16
通讯作者: 李世波,E-mail:lsb0398@126.com
作者简介:柴能明,男,副主任医师,主要从事感染性疾病诊治及流行病学研究,E-mail:327970726@qq.com;谭启龙,男,主管医师,主要从事传染病防制工作,E-mail:339550466@qq.com
基金资助:
浙江省自然科学基金（LY21H100002）；舟山市科技计划项目（2021C31089）

Host animal investigation and etiological analysis of Hantavirus in some areas of Zhoushan Islands, Zhejiang province, China, 2021

CHAI Neng-ming¹, TAN Qi-long², SHU Ji-wei², YE Ling², ZHANG Tong-Jie², LI Shi-bo³, ZHANG Sen⁴

1. Department of Infectious Disease, Daishan First People's Hospital, Zhoushan, Zhejiang 316200, China;
2. Department of Infectious Disease Conirol and Prevention, Daishan Center for Disease Control and Prevention, Zhoushan, Zhejiang 316200, China;
3. Department of Infectious Disease, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, Zhejiang 316000, China;
4. Dinghai Center for Disease Control and Prevention of Zhoushan, Zhoushan, Zhejiang 316000, China

Received:2023-01-12 Online:2023-06-20 Published:2023-06-16
Supported by:
Natural Science Foundation of Zhejiang Province of China (No. LY21H100002); Science and Technology Plan Project of Zhoushan (No. 2021C31089)

摘要/Abstract

摘要： 目的了解浙江省舟山群岛部分地区汉坦病毒宿主动物的种群分布、带病毒情况和病毒基因特征,为防制肾综合征出血热（HFRS）提供依据。方法用夹夜法在舟山群岛的定海和岱山片区捕获小兽,进行种类鉴定,用χ²检验分析鼠密度及鼠种构成；取小兽肺,实时荧光定量聚合酶链式反应检测汉坦病毒核酸,巢式PCR扩增M基因并测定序列,进行基因分型和同源性分析。结果 2021年浙江省舟山群岛定海和岱山片区平均鼠密度为3.42％,野外鼠密度为1.71％,居民区鼠密度为6.16％；捕获小兽6种,野外优势鼠种为黑线姬鼠,居民区优势鼠种为褐家鼠；岱山县本岛、岱山县渔山岛和定海区本岛鼠密度（χ²=108.468,P<0.001）,居民区和野外鼠密度（χ²=68.935,P<0.001）,以及岱山县本岛、岱山县渔山岛和定海区本岛不同鼠种鼠密度（χ²=37.749,P<0.001）之间差异均有统计学意义。13份小兽肺标本检测到汉坦病毒RNA,阳性率为6.70%；成功扩增并测序了3份汉坦病毒RNA提取物M段,3个M基因片段同源性在96.90%～97.10%,属于首尔型病毒（SEOV） S3亚型。结论舟山群岛存在以褐家鼠为传染源的HFRS疫源地,流行型别为SEOV S3亚型；环境改变可能带来自然疫源地变化。

关键词: 宿主动物, 测序分析, 流行病学调查, 监测

Abstract: Objective To investigate the population distribution, virus-carrying status, and virus gene characteristics of host animals for Hantavirus in some areas of Zhoushan Islands, Zhejiang province, China, and to provide evidence for prevention and control of hemorrhagic fever with renal syndrome (HFRS).Methods Small mammals were captured using the night trapping method in Dinghai and Daishan areas of Zhoushan Islands, followed by species identification. The rodent density and species composition were analyzed using the Chi-square test. The small mammal’ lungs were collected to detect the nucleic acid of Hantavirus by quantitative real-time PCR. The M gene was amplified by nested PCR and sequenced for genotyping and homology analysis.Results In Dinghai and Daishan areas of Zhoushan Islands in 2021, the average rodent density was 3.42%, with 1.71% in the wild and 6.16% in residential areas. Six species of small mammals were captured. The dominant species was Apodemus agrarius in the wild and Rattus norvegicus in residential areas. The density of rodents was statistically different between Daishan Island, Yushan Island, and Dinghai Island (χ²=108.468, P<0.001), between the wild and residential areas (χ²=68.935, P<0.001), and between different species (χ²=37.749, P<0.001). Hantavirus RNA was detected in 13 small mammals lung specimens, with a positive rate of 6.70%. The M fragments of three Hantavirus RNA extracts were successfully amplified and sequenced. The homology of the three M fragments was 96.90%-97.10%, belonging to Seoul virus (SEOV, S3 subtype).Conclusions There are HFRS foci with R. norvegicus as the source of infection in Zhoushan Islands, where SEOV S3 subtype is the epidemic strain. Environmental changes may alter natural foci.

Key words: Host animal, Sequencing analysis, Epidemiological investigation, Surveillance

中图分类号:

R446.5

柴能明, 谭启龙, 舒纪为, 叶凌, 张彤杰, 李世波, 张森. 浙江省舟山群岛部分地区2021年汉坦病毒宿主调查与病原学分析[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 326-330.

CHAI Neng-ming, TAN Qi-long, SHU Ji-wei, YE Ling, ZHANG Tong-Jie, LI Shi-bo, ZHANG Sen. Host animal investigation and etiological analysis of Hantavirus in some areas of Zhoushan Islands, Zhejiang province, China, 2021[J]. Chinese Journal of Vector Biology and Control, 2023, 34(3): 326-330.

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浙江省舟山群岛部分地区2021年汉坦病毒宿主调查与病原学分析

Host animal investigation and etiological analysis of Hantavirus in some areas of Zhoushan Islands, Zhejiang province, China, 2021

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