河南省禹州地区白纹伊蚊击倒抗性基因突变检测

doi:10.11853/j.issn.1003.8280.2023.03.004

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (3): 303-307.DOI: 10.11853/j.issn.1003.8280.2023.03.004

河南省禹州地区白纹伊蚊击倒抗性基因突变检测

母群征¹, 华栋栋², 李文玉³, 周欣欣⁴, 伦辛畅¹, 李贵昌¹, 王君¹, 宋秀平¹, 刘起勇¹, 孟凤霞¹

1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
2. 山东大学公共卫生学院, 山东济南 250000;
3. 宁夏回族自治区疾病预防控制中心, 宁夏银川 750000;
4. 北京市大兴区疾病预防控制中心, 北京 102600

收稿日期:2023-02-13 出版日期:2023-06-20 发布日期:2023-06-16
通讯作者: 孟凤霞,E-mail:mengfengxia@icdc.cn
作者简介:母群征,男,在读硕士,主要从事病媒生物及相关传染病控制工作,E-mail:15165480590@163.com
基金资助:
国家科技重大专项（2018ZX101010002-002）

Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan province, China

MU Qun-zheng¹, HUA Dong-dong², LI Wen-yu³, ZHOU Xin-xin⁴, LUN Xin-chang¹, LI Gui-chang¹, WANG Jun¹, SONG Xiu-ping¹, LIU Qi-yong¹, MENG Feng-xia¹

1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
2. School of Public Health, Shandong University, Ji'nan, Shandong 250000, China;
3. Ningxia Center for Disease Prevention and Control, Yinchuan, Ningxia 750000, China;
4. Daxing District Center for Disease Control and Prevention of Beijing, Beijing 102600, China

Received:2023-02-13 Online:2023-06-20 Published:2023-06-16
Supported by:
National Science and Technology Major Project of China (No. 2018ZX101010002-002)

摘要/Abstract

摘要： 目的了解河南省禹州地区白纹伊蚊击倒抗性基因突变情况,为白纹伊蚊防控提供依据。方法 2020和2022年9月中旬在禹州地区利用双层叠帐法捕获蚊虫；依据形态特征鉴定蚊虫种类,提取白纹伊蚊DNA,通过测序法对白纹伊蚊击倒抗性基因进行检测。结果本次调查每年捕获40只共计80只白纹伊蚊。测序结果表明白纹伊蚊3个击倒抗性基因位点均有发现。1016位点存在由密码子GTA编码的缬氨酸（Val,V）突变为GGA编码的甘氨酸（Gly,G）；1532位点存在由密码子ATC编码的异亮氨酸（Ile,I）突变为ACC编码的苏氨酸（Thr,T）；1534位点突变等位基因型最多,包括由密码子TTC（Phe,F）编码的苯丙氨酸突变为TCC编码的丝氨酸（Ser,S）或TTA/CTC编码的亮氨酸（Leu,L）；3个突变位点共包括10种基因型。3个突变位点均是以野生型纯合子为主,野生/突变型杂合子次之,突变型纯合子所占比例最低。共得到11种组合基因型,其中单个位点野生/突变杂合型组合基因型频率最高,为55.00%,3个位点的野生纯合型组合基因型频率为28.75%；双位点野生/突变杂合型基因型频率为8.75%,单个位点突变纯合型基因型频率最低,为7.50%。结论首次在禹州地区开展白纹伊蚊击倒抗性基因突变检测,发现其基因突变率高且突变情况复杂,今后应在该地区开展深入调查研究。

关键词: 白纹伊蚊, 击倒抗性基因, 禹州市

Abstract: Objective To investigate the knockdown resistance gene mutations of Aedes albopictus in Yuzhou, Henan province, China, and to provide a basis for the control of Ae. albopictus.Methods The double-layered mosquito net method was used to capture mosquitoes in Yuzhou in mid-September of 2020 and 2022. Mosquito species was identified based on morphological characteristics. The DNA of Ae. albopictus mosquitoes was extracted for knockdown resistance gene detection by sequencing.Results A total of 80 Ae. albopictus mosquitoes (40 in each year) were captured. The sequencing results showed that three knockdown resistance gene loci were found mutant in Ae. albopictus. The 1016 locus mutated from valine (Val, V) encoded by the codon GTA to glycine (Gly, G) encoded by GGA. The 1532 locus mutated from ATC-encoded isoleucine (Ile, I) to ACC-encoded threonine (Thr, T). Mutations at 1534 were most frequent, from TTC-encoded phenylalanine (Phe, F) to TCC-encoded serine (Ser, S) or Leu (Leu, L) encoded by TTA/CTC. There were 10 genotypes in total for the three mutant sites. For all the three mutant sites, wild-type homozygotes were predominant, followed by wild/mutant heterozygotes and then mutant homozygotes. There were a total of 11 combination genotypes: the frequency of wild/mutant heterozygous combination genotypes at single loci was 55.00%, which was highest; the frequency of wild homozygous combination genotypes at three loci was 28.75%; the frequency of wild/mutant heterozygous genotypes at two loci was 8.75%; and the frequency of mutant homozygous genotypes at single loci was 7.50%, which was lowest.Conclusions It was the first time to carry out knockdown resistance gene mutation detection in Ae. albopictus in Yuzhou, discovering that the mutations were frequent and complex, which requires further research in this area.

Key words: Aedes albopictus, Knockdown resistance gene, Yuzhou

中图分类号:

S443

母群征, 华栋栋, 李文玉, 周欣欣, 伦辛畅, 李贵昌, 王君, 宋秀平, 刘起勇, 孟凤霞. 河南省禹州地区白纹伊蚊击倒抗性基因突变检测[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 303-307.

MU Qun-zheng, HUA Dong-dong, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, LI Gui-chang, WANG Jun, SONG Xiu-ping, LIU Qi-yong, MENG Feng-xia. Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan province, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(3): 303-307.

参考文献

[1] Wu TT,Wu ZD,Li YP. Dengue fever and dengue virus in the People's Republic of China[J]. Rev Med Virol,2022,32(1):e2245. DOI:10.1002/rmv.2245.
[2] Lun XC,Wang YG,Zhao CC,et al. Epidemiological characteristics and temporal-spatial analysis of overseas imported dengue fever cases in outbreak provinces of China,2005-2019[J]. Infect Dis Poverty,2022,11(1):12. DOI:10.1186/s40249-022-00937-5.
[3] Yue YJ,Liu QY,Liu XB,et al. Dengue fever in Mainland China,2005-2020:A descriptive analysis of dengue cases and Aedes data[J]. Int J Environ Res Public Health,2022,19(7):3910. DOI:10.3390/ijerph19073910.
[4] 赵春春. 我国白纹伊蚊抗药性及kdr基因分布研究[D]. 北京:中国疾病预防控制中心,2019.Zhao CC. Study on insectcides resistance and kdr gene distribution of Aedes albopictus in China[D]. Beijing:Chinese Center for Disease Control and Prevention,2019. (in Chinese)
[5] 孟凤霞,王义冠,冯磊,等. 我国登革热疫情防控与媒介伊蚊的综合治理[J]. 中国媒介生物学及控制杂志,2015,26(1):4-10. DOI:10.11853/j.issn.1003.4692.2015.01.002.Meng FX,Wang YG,Feng L,et al. Review on dengue prevention and control and integrated mosquito management in China[J]. Chin J Vector Biol Control,2015,26(1):4-10. DOI:10.11853/j.issn.1003.4692.2015.01.002.(in Chinese)
[6] Dalpadado R,Gunathilaka N,Amarasinghe D,et al. A challenge for a unique dengue vector control programme:Assessment of the spatial variation of insecticide resistance status amongst Aedes aegypti and Ae. albopictus populations in Gampaha district,Sri Lanka[J]. Biomed Res Int,2021,2021:6619175. DOI:10.1155/2021/6619175.
[7] Kasai S,Caputo B,Tsunoda T,et al. First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam),2016:A new emerging threat to controlling arboviral diseases[J]. Euro Surveill,2019,24(5):1700847. DOI:10.2807/1560-7917.ES.2019.24.5.1700847.
[8] Auteri M,La Russa F,Blanda V,et al. Insecticide resistance associated with kdr mutations in Aedes albopictus:An update on worldwide evidences[J]. Biomed Res Int,2018,2018:3098575. DOI:10.1155/2018/3098575.
[9] 周欣欣,李芬,段文波,等. 白纹伊蚊电压门控钠离子通道基因克隆及其生物信息学分析[J]. 中国媒介生物学及控制杂志,2021,32(6):672-679. DOI:10.11853/j.issn.1003.8280. 2021.06.004.Zhou XX,Li F,Duan WB,et al. Cloning and bioinformatics analysis of voltage-gated sodium channel gene of Aedes albopictus[J]. Chin J Vector Biol Control,2021,32(6):672-679. DOI:10.11853/j.issn.1003.8280.2021.06.004.(in Chinese)
[10] Kasai S,Ng LC,Lam-Phua SG,et al. First detection of a putative knockdown resistance gene in major mosquito vector,Aedes albopictus[J]. Jpn J Infect Dis,2011,64(3):217-221.
[11] 朱彩英. 中国白纹伊蚊击倒抗性基因突变及检测技术研究[D]. 北京:中国疾病预防控制中心,2020. DOI:10.27511/d.cnki.gzyyy.2020.000116.Zhu CY. Study on knockdown resistance gene mutation and detection technique of Aedes albopictus in China[D]. Beijing:Chinese Center for Disease Control and Prevention,2020. DOI:10.27511/d.cnki.gzyyy.2020.000116.(in Chinese)
[12] Wu YY,Liu QM,Qi YP,et al. Knockdown resistance (kdr) mutations I1532T and F1534S were identified in Aedes albopictus field populations in Zhejiang province,central China[J]. Front Cell Infect Microbiol,2021,11:702081. DOI:10.3389/fcimb. 2021.702081.
[13] Zhou XJ,Yang C,Liu N,et al. Knockdown resistance (kdr) mutations within seventeen field populations of Aedes albopictus from Beijing China:First report of a novel V1016G mutation and evolutionary origins of kdr haplotypes[J]. Parasit Vectors,2019,12(1):180. DOI:10.1186/s13071-019-3423-x.
[14] 马红霞,杜燕华,黄学勇,等. 河南省2013年登革热暴发的登革热病毒基因组序列测定及分析[J]. 中华流行病学杂志,2015,36(10):1185-1186. DOI:10.3760/cma.j.issn.0254-6450. 2015.10.032.Ma HX,Du YH,Huang XY,et al. Analysis of the genome sequences of dengue virus caused an outbreak of dengue fever in Henan province,2013[J]. Chin J Epidemiol,2015,36(10):1185-1186. DOI:10.3760/cma.j.issn.0254-6450.2015.10.032.(in Chinese)
[15] 杜燕华,王若琳,李东晓,等. 河南省2018年输入性登革热的病原监测分析[J]. 中华流行病学杂志,2020,41(4):542-546. DOI:10.3760/cma.j.cn112338-20190614-00437.Du YH,Wang RL,Li DX,et al. Surveillance on the pathogen of imported dengue fever in Henan province,2018[J]. Chin J Epidemiol,2020,41(4):542-546. DOI:10.3760/cma.j.cn112338-20190614-00437.(in Chinese)
[16] 王福春. 我国登革热流行概况与预防控制措施研究进展[J]. 职业与健康,2018,34(12):1717-1721. DOI:10.13329/j.cnki.zyyjk.2018.0392.Wang FC. Epidemic situation and prevention and control measures of dengue fever in China[J]. Occup Health,2018,34(12):1717-1721. DOI:10.13329/j.cnki.zyyjk.2018.0392.(in Chinese)
[17] 王永亮,张勤,苗丽,等. 河南口岸81例输入性登革热流行病学分析及防控对策[J]. 口岸卫生控制,2017,22(5):49-52. DOI:10.3969/j.issn.1008-5777.2017.05.017.Wang YL,Zhang Q,Miao L,et al. Epidemiological analysis and prevention measures of 81 imported dengue cases at Henan port[J]. Port Health Control,2017,22(5):49-52. DOI:10.3969/j.issn.1008-5777.2017.05.017.(in Chinese)
[18] 王永亮,李帅,高志华. 河南口岸3例输入性登革热实验室鉴定及回顾调查[J]. 口岸卫生控制,2018,23(3):53-55,58. DOI:10.3969/j.issn.1008-5777.2018.03.015.Wang YL,Li S,Gao ZH. Laboratory identification of 3 imported dengue fever cases together with their retrospective investigation in Henan port[J]. Port Health Control,2018,23(3):53-55,58. DOI:10.3969/j.issn.1008-5777.2018.03.015.(in Chinese)
[19] 赵宁,郭玉红,吴海霞,等. 2019年全国媒介蚊虫监测报告[J]. 中国媒介生物学及控制杂志,2020,31(4):395-400,406. DOI:10.11853/j.issn.1003.8280.2020.04.003.Zhao N,Guo YH,Wu HX,et al. National vector surveillance report on mosquitoes in China,2019[J]. Chin J Vector Biol Control,2020,31(4):395-400,406. DOI:10.11853/j.issn. 1003.8280.2020.04.003.(in Chinese)
[20] 汪峰,李洁,赵奇,等. 2014-2020年河南省禹州市登革热媒介伊蚊监测结果[J]. 河南预防医学杂志,2022,33(7):529-531. DOI:10.13515/j.cnki.hnjpm.1006-8414.2022.07.013.Wang F,Li J,Zhao Q,et al. Surveillance results of dengue vector Aedes larvae density in Yuzhou of Henan in 2014-2020[J]. Henan J Prev Med,2022,33(7):529-531. DOI:10.13515/j.cnki.hnjpm.1006-8414.2022.07.013.(in Chinese)
[21] 赵春春,周欣欣,李文玉,等. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究[J]. 中国媒介生物学及控制杂志,2022,33(1):30-37. DOI:10.11853/j.issn.1003.8280. 2022.01.006.Zhao CC,Zhou XX,Li WY,et al. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020[J]. Chin J Vector Biol Control,2022,33(1):30-37. DOI:10.11853/j.issn.1003. 8280.2022.01.006.(in Chinese)
[22] 唐振强,岳思宁,赵奇. 2021年河南省白纹伊蚊对杀虫剂抗药性监测[J]. 河南预防医学杂志,2022,33(2):116-118. DOI:10.13515/j.cnki.hnjpm.1006-8414.2022.02.009.Tang ZQ,Yue SN,Zhao Q. Monitoring of insecticide resistance of Aedes albopictus in Henan province in 2021[J]. Henan J Prev Med,2022,33(2):116-118. DOI:10.13515/j.cnki.hnjpm.1006-8414.2022.02.009.(in Chinese)
[23] 韦凌娅,孔庆鑫,王慧敏. 双层叠帐法与BG-trap诱捕器法在登革热应急蚊媒监测中的捕蚊效果比较[J]. 中国媒介生物学及控制杂志,2019,30(1):65-68. DOI:10.11853/j.issn.1003. 8280.2019.01.014.Wei LY,Kong QX,Wang HM. Effectiveness of double mosquito net and BG-trap for emergency vector surveillance during dengue fever epidemics:A comparative study[J]. Chin J Vector Biol Control,2019,30(1):65-68. DOI:10.11853/j.issn.1003.8280. 2019.01.014.(in Chinese)
[24] 陆宝麟,苏龙. 中国伊蚊鉴定手册[M]. 北京:科学出版社,1987:55-56.Lu BL,Su L. Aedes identification manual in China[M]. Beijing:Science Press,1987:55-56. (in Chinese)
[25] 王永亮,李新民,郝宗宇,等. 河南首次输入性登革热暴发流行控制的思考[J]. 河南预防医学杂志,2016,27(5):321-324. DOI:10.13515/j.cnki.hnjpm.1006-8414.2016.05.001.Wang YL,Li XM,Hao ZY,et al. A outbreak of the first imported dengue fever epidemical control in Henan[J]. Henan J Prev Med,2016,27(5):321-324. DOI:10.13515/j.cnki.hnjpm.1006-8414.2016.05.001.(in Chinese)
[26] Chen HM,Zhou QM,Dong HW,et al. The pattern of kdr mutations correlated with the temperature in field populations of Aedes albopictus in China[J]. Parasit Vectors,2021,14(1):406. DOI:10.1186/s13071-021-04906-z.
[27] Plernsub S,Saingamsook J,Yanola J,et al. Additive effect of knockdown resistance mutations,S989P,V1016G and F1534C,in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand[J]. Parasit Vectors,2016,9(1):417. DOI:10.1186/s13071-016-1713-0.
[28] Smith LB,Kasai S,Scott JG. Pyrethroid resistance in Aedes aegypti and Ae. albopictus:Important mosquito vectors of human diseases[J]. Pestic Biochem Physiol,2016,133:1-12. DOI:10.1016/j.pestbp.2016.03.005.
[29] Gao JP,Chen HM,Shi H,et al. Correlation between adult pyrethroid resistance and knockdown resistance (kdr) mutations in Aedes albopictus (Diptera:Culicidae) field populations in China[J]. Infect Dis Poverty,2018,7(1):86. DOI:10.1186/s40249-018-0471-y.
[30] Wei Y,Zheng XL,He S,et al. Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China[J]. Parasit Vectors,2021,14(1):609. DOI:10.1186/s13071-021-05095-5.
[31] Zulfa R,Lo WC,Cheng PC,et al. Updating the insecticide resistance status of Aedes aegypti and Ae. albopictus in Asia:A systematic review and meta-analysis[J]. Trop Med Infect Dis,2022,7(10):306. DOI:10.3390/tropicalmed7100306.

河南省禹州地区白纹伊蚊击倒抗性基因突变检测

Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan province, China

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[11]	李世豪, 王伟, 张静, 李培羽, 李金越, 秦娜. 天津市2019－2021年登革热媒介白纹伊蚊监测分析[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 820-822.
[12]	杨新艳, 钟汶兵, 林怡, 蔡芳, 刘义, 邝芝正. 海口市美舍河沿岸2021年登革热媒介白纹伊蚊监测分析[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 823-827.
[13]	江汉鹏, 饶志云, 凌春燕, 杨兴华, 谢军. 江西省抚州市2021年白纹伊蚊生态学、抗药性监测及卫生杀虫剂使用状况调查[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 828-833.
[14]	曹阳, 韦凌娅, 金慧, 王慧敏, 孔庆鑫. 实验室温度对常规保存白纹伊蚊卵的孵化效果影响研究[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 661-665.
[15]	陈秀洁, 杨小军, 开文龙, 师灿南, 王义冠, 赵春春, 伦辛畅, 孟凤霞. 杀虫剂稀释倍数和雾滴粒径对白纹伊蚊控制效果的影响[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 748-752.