基于mtDNA-COⅠ基因的山东省淡色库蚊种群遗传多样性分析

doi:10.11853/j.issn.1003.8280.2023.03.003

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (3): 298-302.DOI: 10.11853/j.issn.1003.8280.2023.03.003

基于mtDNA-COⅠ基因的山东省淡色库蚊种群遗传多样性分析

臧传慧¹, 李黎明², 刘烁², 公茂庆¹, 王文倩¹, 王奕婷¹, 娄紫薇¹, 类晶晶¹, 程鹏¹, 刘宏美¹

1. 山东第一医科大学(山东省医学科学院), 山东省寄生虫病防治研究所医学昆虫学部, 山东济宁 272033;
2. 济宁市疾病预防控制中心, 山东济宁 272033

收稿日期:2023-01-03 出版日期:2023-06-20 发布日期:2023-06-16
通讯作者: 刘宏美,E-mail:liuhmm163@163.com
作者简介:臧传慧,女,在读硕士,主要从事媒介蚊虫防治研究工作,E-mail::zch18853812382@163.com
基金资助:
国家自然科学基金（81871685）；山东省自然科学基金（ZR2020KH001，ZR2020MC048）

Genetic diversity of Culex pipiens pallens populations in Shandong province, China based on the mtDNA-COⅠgene

ZANG Chuan-hui¹, LI Li-ming², LIU Shuo², GONG Mao-qing¹, WANG Wen-qian¹, WANG Yi-ting¹, LOU Zi-wei¹, LEI Jing-jing¹, CHENG Peng¹, LIU Hong-mei¹

1. Shandong First Medical University & Shandong Academy of Medical Sciences, Department of Medical Entomology, Shandong Institute of Parasitic Diseases, Jining, Shandong 272033, China;
2. Jining City Center for Disease Control and Prevention, Jining, Shandong 272033, China

Received:2023-01-03 Online:2023-06-20 Published:2023-06-16
Supported by:
National Natural Science Foundation of China (No. 81871685); Natural Science Foundation of Shandong Province of China (No. ZR2020KH001, ZR2020MC048)

摘要/Abstract

摘要： 目的探讨山东省淡色库蚊种群遗传多样性、遗传分化和系统发育关系。方法从2020年9月－2022年9月,采集山东省菏泽、德州、聊城、青岛、烟台和日照共6个市的淡色库蚊。从单只雌性成蚊中分别提取基因组DNA,用PCR法扩增线粒体细胞色素C氧化酶亚基Ⅰ（mtDNA-COⅠ）基因序列并测序,获得的序列在GenBank上进行BLAST比对。应用BioEdit 7.0软件对测序结果比对分析；运用DnaSP v6软件分析种群的遗传多样性；采用Arlequin 3.5软件计算种群遗传分化系数和基因流；使用PopART 1.7软件构建单倍型网络图（TCS network法）。结果山东省6个淡色库蚊种群共获得423条序列,其长度均为603 bp。A+T平均含量占69.2%,符合线粒体DNA的AT偏向性。单倍型分析共获得8种单倍型,其中单倍型H01为优势单倍型。mtDNA-COⅠ序列分析结果显示,种群具有丰富的遗传多样性；分子变异等级分析表明淡色库蚊遗传分化主要来自于种群内部；部分种群间存在一定的遗传分化现象。中性检验结果显示：聊城和菏泽淡色库蚊种群近期经历过扩张。结论 mtDNA-COⅠ基因可以作为研究淡色库蚊种群遗传多样性的分子标志。青岛种群遗传发育与其他地理种群相比有一定特殊性。

关键词: 淡色库蚊, 遗传多样性, 山东省, 线粒体细胞色素C氧化酶亚基Ⅰ基因

Abstract: Objective To investigate the genetic diversity, genetic differentiation, and phylogenetic relationship of Culex pipiens pallens populations in Shangdong, China.Methods Cx. pipiens pallens populations were collected in Heze, Dezhou, Liaocheng, Qingdao, Yantai, and Rizhao, Shandong province from September 2020 to September 2022. Genomic DNA was extracted from a single female adult mosquito. The mitochondrial cytochrome c oxidase subunit Ⅰ (mtDNA-COⅠ) gene sequences were amplified by polymerase chain reaction and sequenced afterwards. The acquired sequences were compared with those in the Basic Local Alignment Search Tool on the GenBank. BioEdit 7.0 was used to compare and analyze the sequencing results. DnaSP v6 was used to analyze the genetic diversity of populations. Arlequin 3.5 was used to calculate the genetic differentiation coefficient and gene flow of populations. PopART 1.7 was used to construct the haplotype network (TCS network).Results A total of 423 sequences of 603 bp were obtained from 6 Cx. pipiens pallens populations in Shandong province. The average content of A+T bases was 69.2%, which was consistent with the AT bias of mitochondrial DNA. There were eight haplotypes in the haplotype analysis, of which haplotype H01 was the dominant haplotype. The results of mtDNA-COⅠ sequence analysis showed that the population had rich genetic diversity. The results of molecular variance analysis indicated that the genetic differentiation of Cx. pipiens pallens mainly came from within the population; there was some genetic differentiation among some populations. The results of neutrality test showed that the populations of Cx. pipiens pallens in Liaocheng and Heze experienced expansion recently.Conclusions The mtDNA-COⅠ gene can be used as a molecular marker to study the genetic diversity of Cx. pipiens pallens populations. The genetic development of Qingdao population is special compared with other geographical populations.

Key words: Culex pipiens pallens, Genetic diversity, Shandong province, mtDNA-COⅠ

中图分类号:

Q963

臧传慧, 李黎明, 刘烁, 公茂庆, 王文倩, 王奕婷, 娄紫薇, 类晶晶, 程鹏, 刘宏美. 基于mtDNA-COⅠ基因的山东省淡色库蚊种群遗传多样性分析[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 298-302.

ZANG Chuan-hui, LI Li-ming, LIU Shuo, GONG Mao-qing, WANG Wen-qian, WANG Yi-ting, LOU Zi-wei, LEI Jing-jing, CHENG Peng, LIU Hong-mei. Genetic diversity of Culex pipiens pallens populations in Shandong province, China based on the mtDNA-COⅠgene[J]. Chinese Journal of Vector Biology and Control, 2023, 34(3): 298-302.

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基于mtDNA-COⅠ基因的山东省淡色库蚊种群遗传多样性分析

Genetic diversity of Culex pipiens pallens populations in Shandong province, China based on the mtDNA-COⅠgene

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