白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究

doi:10.11853/j.issn.1003.8280.2023.02.009

中国媒介生物学及控制杂志 ›› 2023, Vol. 34 ›› Issue (2): 196-203.DOI: 10.11853/j.issn.1003.8280.2023.02.009

白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究

华栋栋^1,2, 王磊², 肖迪², 李文玉², 周欣欣², 伦辛畅², 母群征², 刘起勇², 马伟¹, 孟凤霞²

1. 山东大学齐鲁医学院公共卫生学院, 山东济南 250102;
2. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 北京 102206

收稿日期:2022-11-28 出版日期:2023-04-20 发布日期:2023-04-26
通讯作者: 孟凤霞,E-mail：mengfengxia@icdc.cn;马伟,E-mail：weima@sdu.edu.cn
作者简介:华栋栋,女,在读硕士,主要从事白纹伊蚊代谢抗性机制研究,E-mail：hdd20205093@163.com
基金资助:
国家科技重大专项（2018ZX10101002-002）；国家自然科学基金（82073615）

A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China

HUA Dong-dong^1,2, WANG Lei², XIAO Di², LI Wen-yu², ZHOU Xin-xin², LUN Xin-chang², MU Qun-zheng², LIU Qi-yong², MA Wei¹, MENG Feng-xia²

1. School of Public Health, Cheeloo College Medicine, Shandong University, Ji'nan, Shangdong 250102, China;
2. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received:2022-11-28 Online:2023-04-20 Published:2023-04-26
Supported by:
National Science and Technology Major Project of China (No. 2018ZX10101002-002);National Natural Science Foundation of China (No. 82073615)

摘要/Abstract

摘要： 目的对白纹伊蚊昌平种群高效氯氰菊酯抗性个体与敏感个体之间的差异表达蛋白进行生物信息学分析，研究白纹伊蚊高效氯氰菊酯代谢抗性机制，为白纹伊蚊抗药性以及杀虫剂的合理使用提供理论依据。方法通过生物学测定筛选出白纹伊蚊昌平种群高效氯氰菊酯抗性个体与敏感个体，提取总蛋白进行液相色谱串联质谱（LC-MS/MS）分析，通过生物信息学分析找到差异表达蛋白，并在mRNA水平上进行验证。结果生物信息学分析共鉴定到1 861个蛋白，其中差异表达蛋白有383个，在抗性个体体内上调表达的有213个，下调表达的170个。选择京都基因与基因组百科全书（KEGG）通路富集分析中显著性P<0.05的功能通路中与药物代谢相关的8个差异表达蛋白进行验证，鉴定到丙氨酸氨基转移酶、尿苷酸-胞苷酸（UMP-CMP）激酶、谷胱甘肽S-转移酶（GST）在抗性个体体内上调表达。结论白纹伊蚊抗性个体与敏感个体之间存在差异表达蛋白，其中丙氨酸氨基转移酶、UMP-CMP激酶、GST在抗性群体中上调表达，可能与高效氯氰菊酯代谢抗性相关。

关键词: 白纹伊蚊, 杀虫剂抗性, 比较蛋白质组学

Abstract: Objective To perform bioinformatics analysis of differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Aedes albopictus population in Changping, Beijing, China, to study the metabolic mechanism of beta-cypermethrin resistance in Ae. albopictus, and to provide theoretical a basis for the rational use of insecticides according to the drug resistance of Ae. albopictus. Methods Beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus population in Changping were selected by biological assay. Total proteins were extracted for LC-MS/MS analysis. Differentially expressed proteins were identified by bioinformatics analysis and confirmed at the mRNA level. Results A total of 1 861 proteins were identified by bioinformatics analysis, of which 383 were differentially expressed, including 213 up-regulated and 170 down-regulated proteins in resistant individuals. Eight differentially expressed proteins related to drug metabolism according to KEGG pathway enrichment analysis (P<0.05) were selected for validation at the mRNA level. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase were up-regulated in resistant individuals. Conclusions There are differentially expressed proteins between beta-cypermethrin-resistant and -sensitive individuals of Ae. albopictus. Alanine aminotransferase, UMP-CMP kinase, and glutathione S-transferase are up-regulated in resistant individuals, which may be associated with beta-cypermethrin resistance.

Key words: Aedes albopictus, Insecticide resistance, Comparative proteomics

中图分类号:

华栋栋, 王磊, 肖迪, 李文玉, 周欣欣, 伦辛畅, 母群征, 刘起勇, 马伟, 孟凤霞. 白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究[J]. 中国媒介生物学及控制杂志, 2023, 34(2): 196-203.

HUA Dong-dong, WANG Lei, XIAO Di, LI Wen-yu, ZHOU Xin-xin, LUN Xin-chang, MU Qun-zheng, LIU Qi-yong, MA Wei, MENG Feng-xia. A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2023, 34(2): 196-203.

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白纹伊蚊昌平种群高效氯氰菊酯抗性的比较蛋白质组学研究

A comparative proteomics analysis of beta-cypermethrin resistance in Aedes albopictus population in Changping, Beijing, China

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