上海地区不同生境游离蜱分布调查

doi:10.11853/j.issn.1003.8280.2022.06.022

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (6): 879-887.DOI: 10.11853/j.issn.1003.8280.2022.06.022

上海地区不同生境游离蜱分布调查

曾文博¹, 李中秋¹, 魏子昕¹, 程东慧¹, 杨丽敏¹, 方圆^1,2, 张仪^1,2

1. 中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心), 国家卫生健康委员会寄生虫病原与媒介生物学重点实验室, 世界卫生组织热带病合作中心, 国家级热带病国际联合研究中心, 上海 200025;
2. 上海交通大学医学院, 国家热带病研究中心全球健康学院, 上海 200025

收稿日期:2022-08-18 出版日期:2022-12-20 发布日期:2022-12-09
通讯作者: 张仪,E-mail:zhangyi@nipd.chinacdc.cn
作者简介:曾文博,男,在读硕士,从事医学媒介生物防制研究,E-mail:wenbozenger@163.com
基金资助:
上海市第五轮公共卫生体系建设三年行动计划重点学科项目（GWV-10.1-XK13）；国家科技基础资源调查专项（2017FY101203）

An investigation of questing ticks collected from different habitats in Shanghai, China

ZENG Wen-bo¹, LI Zhong-qiu¹, WEI Zi-xin¹, CHENG Dong-hui¹, YANG Li-min¹, FANG Yuan^1,2, ZHANG Yi^1,2

1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China;
2. School of Global Health, Shanghai Jiao Tong University School of Medicine and Chinese Center for Tropical Diseases Research, Shanghai 200025, China

Received:2022-08-18 Online:2022-12-20 Published:2022-12-09
Supported by:
Fifth Round of Three-year Action for Public Health System Construction in Shanghai (No. GWV-10.1-XK13); Special Foundation of Basic Science and Technology Resources Survey of Ministry of Science and Technology of China (No. 2017FY101203)

摘要/Abstract

摘要： 目的调查上海地区不同地点、不同生境游离媒介蜱的种类及分布，为蜱媒疾病防制提供依据。方法采用布旗法，于2021年4－7和9－11月在松江、黄浦、崇明、嘉定、宝山、静安、普陀和闵行区等13个调查点采集植被中的游离蜱；形态鉴定后扩增16S rRNA和细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列，利用获得的序列鉴定蜱种并构建系统发育树。结果共采获游离蜱1 937只，隶属2属3种，分别为血蜱属的褐黄血蜱、长角血蜱和扇头蜱属的微小扇头蜱，分别占采获总数的92.36%、6.66%和0.98%。山坡林地采集褐黄血蜱和微小扇头蜱2种400只，占20.65%；公园/景区采集褐黄血蜱和长角血蜱2种1 341只，占69.23%；农村荒地采集褐黄血蜱1种82只，占4.23%；城市绿地采集褐黄血蜱1种114只，占5.89%。系统进化树显示，采获的同一蜱种位于同一进化分支上。结论上海地区不同生境中游离蜱共有3种，不同生境中蜱的种类和数量分布不同，褐黄血蜱为各类生境中的优势种。

关键词: 蜱, 分布, 16S rRNA基因, 细胞色素C氧化酶亚基Ⅰ基因, 系统发育分析

Abstract: Objective To investigate tick species and distribution in different locations and habitats in Shanghai, China, and to provide basic data for the prevention and control of tick-borne diseases. Methods From April to November, except August, in 2021, the drag-flag method was used to collect questing ticks in vegetation at 13 investigation sites in Songjiang, Huangpu, Chongming, Jiading, Baoshan, Jing’an, Putuo, and Minhang districts. After morphological identification, the 16S rRNA and cytochrome c oxidase subunit Ⅰ (COⅠ) gene were amplified to identify the tick species and construct a phylogenetic tree. Results A total of 1 937 questing ticks were collected, which belonged to 3 species from 2 genera, i.e., Haemaphysalis flava (92.36%), H. longicornis (6.66%), and Rhipicephalus microplus (0.98%). Specifically, 400 ticks (20.65%) belonging to H. flava and R. microplus were collected from wooded hillsides; 1 341 (69.23%) belonging to H. flava and H. longicornis were collected from parks and scenic spots; 82 (4.23%) belonging to H. flava were collected from rural wild lands; 114 (5.89%) belonging to H. flava were collected from urban green lands. The phylogenetic tree showed that the collected same tick species were in the same evolutionary branch. Conclusion There are three species of questing ticks in different habitats in Shanghai. The species and number of ticks vary across habitats. H. flava is the dominant species in each type of habitat.

Key words: Tick, Distribution, 16S rRNA gene, COⅠ gene, Phylogenetic analysis

中图分类号:

R384.4

曾文博, 李中秋, 魏子昕, 程东慧, 杨丽敏, 方圆, 张仪. 上海地区不同生境游离蜱分布调查[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 879-887.

ZENG Wen-bo, LI Zhong-qiu, WEI Zi-xin, CHENG Dong-hui, YANG Li-min, FANG Yuan, ZHANG Yi. An investigation of questing ticks collected from different habitats in Shanghai, China[J]. Chinese Journal of Vector Biology and Control, 2022, 33(6): 879-887.

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上海地区不同生境游离蜱分布调查

An investigation of questing ticks collected from different habitats in Shanghai, China

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