中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (6): 800-804.DOI: 10.11853/j.issn.1003.8280.2022.06.006

• 媒介生物监测 • 上一篇    下一篇

山东省部分地区2020-2021年小兽携带鼠传病原体的调查

韩英男, 刘言, 孙钦同, 孟蔚, 孙林, 赖世宏, 王学军, 康殿民   

  1. 山东省疾病预防控制中心消毒与病媒生物防制所, 山东 济南 250014
  • 收稿日期:2022-07-21 出版日期:2022-12-20 发布日期:2022-12-09
  • 通讯作者: 王学军,E-mail:bmfzs@126.com
  • 作者简介:韩英男,女,硕士,主管技师,主要从事媒介生物监测与控制工作,E-mail:747780760@qq.com
  • 基金资助:
    国家科技重大专项(2017ZX100303404);山东省医药卫生科技发展计划(202112070737)

An investigation of rodent-borne pathogens in small mammals in some areas of Shandong province, China

HAN Ying-nan, LIU Yan, SUN Qin-tong, MENG Wei, SUN Lin, LAI Shi-hong, WANG Xue-jun, KANG Dian-min   

  1. Institute of Disinfection & Vector Borne Disease Control, Shandong Provincial Center for Disease Control and Prevention, Ji'nan, Shandong 250014, China
  • Received:2022-07-21 Online:2022-12-20 Published:2022-12-09
  • Supported by:
    National Science and Technology Major Project of China (No. 2017ZX100303404); Medical Health Science and Technology Program of Shandong Province (No. 202112070737)

摘要: 目的 调查山东省部分地区小兽携带4种鼠传病原体情况,为鼠传疾病防制提供理论依据。方法 2020-2021年在山东省东营、潍坊、威海、临沂、滨州和菏泽市采集小兽肝、肾、脾和肺组织样本,应用TaqMan探针荧光PCR法检测钩端螺旋体(钩体)、巴尔通体,应用TaqMan探针反转录荧光定量聚合酶链式反应(RT-qPCR)法检测汉坦病毒、发热伴血小板减少综合征病毒(SFTSV)。采用Excel 2016和SPSS 20.0软件进行统计分析。结果 共捕获小兽973只,其中褐家鼠454只,小家鼠441只,黄胸鼠21只,黑线姬鼠52只,鼩鼱4只,小仓鼠1只。动物组织检测结果显示,肺组织汉坦病毒阳性26份(2.67%),肝、肾、脾组织钩体阳性13份(1.34%)、巴尔通体阳性36份(3.70%),未检出SFTSV。褐家鼠的汉坦病毒和巴尔通体复合感染率为0.62%。结论 山东省小兽存在汉坦病毒、钩体、巴尔通体的感染,应加强小兽携带鼠传病原体监测,优化鼠传疾病的防制工作,降低人群感染风险。

关键词: 小兽, 汉坦病毒, 钩端螺旋体, 巴尔通体, TaqMan探针

Abstract: Objective To investigate the prevalence of 4 rodent-borne pathogens in small mammals in some areas of Shandong province, China, and to provide a scientific basis for the prevention and control of rodent-borne diseases. Methods From 2020 to 2021, small mammals’ liver, kidney, spleen, and lung samples were collected in Dongying, Weifang, Weihai, Linyi, Binzhou, and Heze of Shandong province. TaqMan fluorescent probe-based real-time polymerase chain reaction (PCR) was used to detect Leptospira and Bartonella. TaqMan fluorescent probe-based real-time reverse transcription PCR (RT-qPCR) was used to detect hantavirus and severe fever with thrombocytopenia syndrome virus (SFTSV). Excel 2016 and SPSS 20.0 softwares were used for statistical analysis. Results A total of 973 small mammals were collected, including 454 Rattus norvegicus, 441 Mus musculus, 21 R. tanezumi, 52 Apodemus agrarius, 4 Sorex spp., and 1 species of Cricetulus. The assay of lung tissues showed that 26 (2.67%) were positive for hantavirus. The assay of liver, spleen, and kidney tissues showed that 13 (1.34%) were positive for Leptospira and 36 (3.70%) were positive for Bartonella. No SFTSV was detected. The co-infection rate with hantavirus and Bartonella was 0.62% in R. norvegicus. Conclusion Small mammals in Shandong province carry a variety of pathogens including hantavirus, Leptospira, and Bartonella. It is necessary to strengthen the rodent-borne pathogen surveillance in small mammals and optimize the prevention and control of rodent-borne diseases to reduce the risk of human infection.

Key words: Small mammal, Hantavirus, Leptospira, Bartonella, TaqMan probe

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