不同浓度大蒜素长效驱避剂对长角血蜱幼蜱的驱避效果研究

doi:10.11853/j.issn.1003.8280.2022.05.007

摘要/Abstract

摘要： 目的研究大蒜素对长角血蜱幼蜱的驱避效果及其长效驱避剂的作用时间。方法以长角血蜱幼蜱为测试对象，利用滤纸片体外驱避装置比较研究0.5、1.0、1.5、2.0、3.0、4.0 mg/ml 6种浓度大蒜素溶液对长角血蜱幼蜱的驱避效果，并计算半数驱避浓度（RC₅₀）、全数驱避浓度（RC₁₀₀）；同时使用Kruskal-Wallis H检验研究不同浓度不同剂型大蒜素与3种市售驱避剂（1%驱蚊酯长效驱避剂、OFF驱蚊液、六神驱蚊花露水）每48 h的驱避效果，确定其作用时间。结果大蒜素浓度在0.5～4.0 mg/ml内，随着浓度的增加，其对长角血蜱幼蜱的驱避率也逐渐上升，在4.0 mg/ml时达到最大，为100%，RC₅₀及RC₁₀₀分别为1.79和3.52 mg/ml；0.5%、1.0%、3.0%大蒜素长效驱避剂的驱避活性在10 d内显著大于各自浓度水乳液处理组（均P<0.05），与市售驱避剂相比，1.0%、3.0%大蒜素长效驱避剂在12 d内对长角血蜱幼蜱的驱避活性更好。结论 0.5%、1.0%、3.0%大蒜素长效驱避剂的最长作用时间分别为12、14和16 d，有效保护时间分别为2、4和6 d，驱避效果优于市售驱避剂。

关键词: 大蒜素, 驱避试验, 驱避剂, 长角血蜱, 幼蜱

Abstract: Objective To study the repellent effect of allicin on larvae of Haemaphysalis longicornis and the duration of action of allicin long-acting repellent.Methods The repellent effect of allicin solution (0.5,1.0,1.5,2.0,3.0,and 4.0 mg/ml) on larvae of H. longicornis was studied using an in vitro filter paper repellent device,and the repellent concentration 50(RC₅₀) and repellent concentration 100(RC₁₀₀) were calculated.The Kruskal-Wallis H test was used to compare the repellent effect every 48 h between different concentrations and dosage forms of allicin and three commercially available repellents (1% repellent ester long-lasting repellent,OFF repellent liquid,and Liushen Florida Water) and to determine its duration of action.Results When the concentration of allicin ranged from 0.5 mg/ml to 4.0 mg/ml,the repellent rate of allicin to larvae of H. longicornis increased gradually with the increase in concentration,and reached the maximum (100%) at 4.0 mg/ml;the RC₅₀ and RC₁₀₀ were 1.79 mg/ml and 3.52 mg/ml,respectively.The repellent efficacy of 0.5%,1.0%,and 3.0% allicin long-acting repellents was significantly higher than that of the respective water emulsion treatment group within 10 days (all P<0.05).Compared with the commercially available repellents,1.0% and 3.0% allicin long-acting repellents showed a better repellent activity against larvae of H. longicornis within 12 days.Conclusion 0.5%,1.0%,and 3.0% allicin long-acting repellents have the longest duration of action of 12,14,and 16 days,respectively and the effective protection duration of 2,4,and 6 days,respectively,and their repellent effect is better than that of commercially available repellents.

Key words: Allicin, Repellent test, Repellent, Haemaphysalis longicornis, Larva

中图分类号:

赵聪, 张文举, 宁晓冬, 黄斌斌, 菅忆晨, 岳治光, 菅复春, 张龙现, 张素梅, 宁长申. 不同浓度大蒜素长效驱避剂对长角血蜱幼蜱的驱避效果研究[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 648-654.

ZHAO Cong, ZHANG Wen-ju, NING Xiao-dong, HUANG Bin-bin, JIAN Yi-chen, YUE Zhi-guang, JIAN Fu-chun, ZHANG Long-xian, ZHANG Su-mei, NING Chang-shen. Repellent efficacy of allicin long-acting repellents at different concentrations against larvae of Haemaphysalis longicornis[J]. Chinese Journal of Vector Biology and Control, 2022, 33(5): 648-654.

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