黑龙江省桦南县2020年蜱媒病毒调查

doi:10.11853/j.issn.1003.8280.2022.05.005

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (5): 637-641.DOI: 10.11853/j.issn.1003.8280.2022.05.005

黑龙江省桦南县2020年蜱媒病毒调查

李樊¹, 刘凤明², 冯盼盼¹, 王东强³, 付士红¹, 聂凯¹, 殷启凯¹, 何英¹, 殷佳宇¹, 许松涛¹, 包名家², 王环宇¹

1. 中国疾病预防控制中心病毒病预防控制所虫媒室, 传染病预防控制国家重点实验室, 北京 102206;
2. 佳木斯市疾病预防控制中心, 黑龙江佳木斯 154000;
3. 桦南县疾病预防控制中心, 黑龙江佳木斯 154400

收稿日期:2022-04-23 出版日期:2022-10-20 发布日期:2022-10-14
通讯作者: 王环宇,E-mail:wanghy@ivdc.chinacdc.cn;包名家,E-mail:cdcbmj@126.com
作者简介:李樊,女,博士,副研究员,主要从事虫媒病毒的分离鉴定和遗传变异研究,E-mail:lifan@ivdc.chinacdc.cn;刘凤明,男,主治医师,主要从事媒介生物监测研究,E-mail:15945445933@163.com
基金资助:
国家自然科学基金(31900156);传染病预防控制国家重点实验室重点项目(2015SKLID505);美国NIH项目(U01 AI151810)

Investigation of tick-borne viruses in Huanan county,Heilongjiang province,China,2020

LI Fan¹, LIU Feng-ming², FENG Pan-pan¹, WANG Dong-qiang³, FU Shi-hong¹, NIE Kai¹, YIN Qi-kai¹, HE Ying¹, YIN Jia-yu¹, XU Song-tao¹, BAO Ming-jia², WANG Huan-yu¹

1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Arbovirus, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Jiamusi Center for Disease Control and Prevention, Jiamusi, Heilongjiang 154000, China;
3. Hua'nan Center for Disease Control and Prevention, Jiamusi, Heilongjiang 154400, China

Received:2022-04-23 Online:2022-10-20 Published:2022-10-14
Supported by:
National Natural Science Foundation of China (No.31900156);Development Grant of State Key Laboratory of Infectious Disease Prevention and Control (No.2015SKLID505);The United States National Institutes of Health Foundation (No.U01 AI151810)

摘要/Abstract

摘要： 目的调查黑龙江省佳木斯市桦南县蜱及蜱媒病毒种类。方法于2020年5月在桦南县采集蜱标本，并进行形态学鉴定。利用实时荧光定量PCR方法对采集的蜱标本进行蜱传脑炎病毒（TBEV）、发热伴血小板减少综合征病毒、鄂木斯克出血热病毒、兰加特病毒、波瓦森病毒、阿龙山病毒核酸检测，并使用高通量测序法获得阳性病毒基因组序列。结果共采获890只蜱，包括全沟硬蜱475只（雄蜱210只、雌蜱265只），森林革蜱360只（雄蜱260只、雌蜱100只），嗜群血蜱55只（雄蜱5只、雌蜱50只）。1批（10只蜱混合）标本（JMS20）TBEV核酸阳性，由雌性全沟硬蜱携带。其他病毒核酸全部阴性。系统进化显示，该株蜱传脑炎病毒与远东型TBEV亲缘关系较近。结论佳木斯地区蜱媒样本仍携带蜱传脑炎病毒，研究结果为该地区蜱传病毒病的防控提供了基础数据。

关键词: 蜱, 蜱媒病毒, 蜱传脑炎病毒

Abstract: Objective To investigate the species of ticks and tick-borne viruses in Huanan county,Jiamusi city,Heilongjiang province,China.Methods Ticks were collected in Huanan in May 2020 and identified according to morphological keys.Real-time PCR was used to detect the RNA of viruses in the collected ticks,i.e.,tick-borne encephalitis virus (TBEV),severe fever with thrombocytopenia syndrome virus (SFTSV),Omsk hemorrhagic fever virus (OHFV),Langat virus (LGTV),Powassan virus (POWV),and Alongshan virus (ALSV).High-throughput sequencing was used to obtain the genome sequences of detected viruses.Results A total of 890 ticks were collected,including 475 Ixodes persulcatus(210 males and 265 females),360 Dermacentor silvarum(260 males and 100 females),and 55 Haemaphysalis concinna(5 males and 50 females).One batch of tick specimens (n=10;JMS20) was positive for TBEV,which was carried by female I. persulcatus.Other viruses were not detectable.Phylogenetic analysis showed that this TBEV strain was closely related to the Far Eastern subtype of TBEV.Conclusion Ticks in Jiamusi city still carry TBEV,and our results provide basic data for the prevention and control of tick-borne virus diseases in the region.

Key words: Tick, Tick-borne virus, Tick-borne encephalitis virus

中图分类号:

李樊, 刘凤明, 冯盼盼, 王东强, 付士红, 聂凯, 殷启凯, 何英, 殷佳宇, 许松涛, 包名家, 王环宇. 黑龙江省桦南县2020年蜱媒病毒调查[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 637-641.

LI Fan, LIU Feng-ming, FENG Pan-pan, WANG Dong-qiang, FU Shi-hong, NIE Kai, YIN Qi-kai, HE Ying, YIN Jia-yu, XU Song-tao, BAO Ming-jia, WANG Huan-yu. Investigation of tick-borne viruses in Huanan county,Heilongjiang province,China,2020[J]. Chinese Journal of Vector Biology and Control, 2022, 33(5): 637-641.

参考文献

[1] Boulanger N,Boyer P,Talagrand-Reboul E,et al. Ticks and tick-borne diseases[J]. Méd Mal Infect,2019,49(2):87-97. DOI:10.1016/j.medmal.2019.01.007.
[2] Shi JM,Hu ZH,Deng F,et al. Tick-borne viruses[J]. Virol Sin,2018,33(1):21-43. DOI:10.1007/s12250-018-0019-0.
[3] 石华,王玥,韩华,等.蜱媒疾病风险评估中标本采集方法的探讨[J].中华卫生杀虫药械,2013,19(4):308-310. DOI:10.19821/j.1671-2781.2013.04.010. Shi H,Wang Y,Han H,et al. Discussion of methods for specimen collection in the tick-borne risk evaluation[J]. Chin J Hyg Insect Equip,2013,19(4):308-310. DOI:10.19821/j.1671-2781.2013. 04.010.(in Chinese)
[4] 王倩莹,刘凤明,付士红,等.黑龙江边境地区蜱虫携带病原体调查[J].中华实验和临床病毒学杂志,2019,33(5):504-508. DOI:10.3760/cma.j.issn.1003-9279.2019.05.013. Wang QY,Liu FM,Fu SH,et al. Investigation of tick-borne pathogens in Heilongjiang border area[J]. Chin J Exp Clin Viral,2019,33(5):504-508. DOI:10.3760/cma.j.issn.1003-9279. 2019.05.013.(in Chinese)
[5] Langmead B,Salzberg SL. Fast gapped-read alignment with Bowtie 2[J]. Nat Methods,2012,9(4):357-359. DOI:10.1038/nmeth.1923.
[6] Rozewicki J,Li SL,Amada KM,et al. MAFFT-DASH:Integrated protein sequence and structural alignment[J]. Nucleic Acids Res,2019,47(W1):W5-W10. DOI:10.1093/nar/gkz342.
[7] Jia N,Wang JF,Shi WQ,et al. Large-scale comparative analyses of tick genomes elucidate their genetic diversity and vector capacities[J]. Cell,2020,182(5):1328-1340.e13. DOI:10. 1016/j.cell.2020.07.023.
[8] Varlacher JF,Hägglund S,Juremalm M,et al. Tick-borne encephalitis[J]. Rev Sci Tech,2015,34(2):453-466. DOI:10.20506/rst.34.2.2371.
[9] Chen XJ,Li F,Yin QK,et al. Epidemiology of tick-borne encephalitis in China,2007-2018[J]. PLoS One,2019,14(12):e0226712. DOI:10.1371/journal.pone.0226712.
[10] 吕志,梁国栋.中国蜱传脑炎研究进展[J].中华流行病学杂志,2009,30(6):641-643. DOI:10.3760/cma.j.issn.0254-6450. 2009.06.026. Lyu Z,Liang GD. Advances in research on tick-borne encephalitis in China[J]. Chin J Epidemiol,2009,30(6):641-643. DOI:10.3760/cma.j.issn.0254-6450.2009.06.026.(in Chinese)
[11] Dumpis U,Crook D,Oksi J. Tick-borne encephalitis[J]. Clin Infect Dis,1999,28(4):882-890. DOI:10.1086/515195.
[12] Süss J. Tick-borne encephalitis 2010:Epidemiology,risk areas,and virus strains in Europe and Asia-an overview[J]. Ticks Tick Borne Dis,2011,2(1):2-15. DOI:10.1016/j.ttbdis.2010.10.007.
[13] Xing Y,Schmitt HJ,Arguedas A,et al. Tick-borne encephalitis in China:A review of epidemiology and vaccines[J]. Vaccine,2017,35(9):1227-1237. DOI:10.1016/j.vaccine.2017.01.015.
[14] Subbotina EL,Loktev VB. Molecular evolution of the tick-borne encephalitis and Powassan viruses[J]. Mol Biol,2012,46(1):75-84. DOI:10.1134/S0026893311060148.
[15] Deviatkin AA,Karganova GG,Vakulenko YA,et al. TBEV subtyping in terms of genetic distance[J]. Viruses,2020,12(11):1240. DOI:10.3390/v12111240.
[16] Zhan JB,Wang Q,Cheng J,et al. Current status of severe fever with thrombocytopenia syndrome in China[J]. Virol Sin,2017,32(1):51-62. DOI:10.1007/s12250-016-3931-1.
[17] Wang ZD,Wang B,Wei F,et al. A new segmented virus associated with human febrile illness in China[J]. N Engl J Med,2019,380(22):2116-2125. DOI:10.1056/NEJMoa1805068.
[18] Kuivanen S,Levanov L,Kareinen L,et al. Detection of novel tick-borne pathogen,Alongshan virus,in Ixodes ricinus ticks,south-eastern Finland,2019[J]. Euro Surveill,2019,24(27):1900394. DOI:10.2807/1560-7917.ES.2019.24.27.1900394.
[19] Kholodilov IS,Litov AG,Klimentov AS,et al. Isolation and characterisation of Alongshan virus in Russia[J]. Viruses,2020,12(4):362. DOI:10.3390/v12040362.

黑龙江省桦南县2020年蜱媒病毒调查

Investigation of tick-borne viruses in Huanan county,Heilongjiang province,China,2020

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	崔莹莹, 王俊刚, 孙琬琬, 王君, 吴海霞, 刘起勇. 长角血蜱哈氏器对几种气味物质的触角电位试验[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 622-626.
[2]	郑卫青, 付仁龙, 陶卉英, 刘仰青, 陈盛恩. 实验室长角血蜱吸食小鼠血方法与评价[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 627-631.
[3]	高杨, 许士奇, 王丽娜, 郭文平, 刘铭. 河北省塞罕坝国家级自然保护区蜱类调查研究[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 632-636.
[4]	段立科, 侯学霞, 张琳, 包子豪, 刘泽梁, 施淇源, 周海健, 董爱英, 郝琴. 内蒙古阿尔山地区游离蜱携带伯氏疏螺旋体的检测及基因分型研究[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 642-647.
[5]	赵聪, 张文举, 宁晓冬, 黄斌斌, 菅忆晨, 岳治光, 菅复春, 张龙现, 张素梅, 宁长申. 不同浓度大蒜素长效驱避剂对长角血蜱幼蜱的驱避效果研究[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 648-654.
[6]	董红猛, 武亚雪, 胡永红. 抗蜱亚单位疫苗作用途径解析[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 655-660.
[7]	王晗, 曹淦, 孙刚, 杨晓, 田诚. 我国常见蜱传疾病的流行特征和检测方法研究进展[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 765-770.
[8]	王金娜, 李天奇, 罗明宇, 吴瑜燕, 刘钦梅, 龚震宇. 浙江省2017－2020年蜱生态学监测结果分析[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 466-470.
[9]	张乾通, 孙继民, 凌锋, 施旭光, 任江萍, 郭颂, 张蓉, 刘营. 浙江省2021年发热伴血小板减少综合征报告病例及蜱媒监测结果分析[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 485-488.
[10]	朱心红, 吴因平, 李天奇, 王金娜, 侯娟. 浙江省义乌市2021年3种不同生境中山羊寄生蜱类调查[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 493-498.
[11]	孟娇, 陈醒醒, 张霞, 余福勋, 吴家红, 杨光红, 江佳富, 孙毅, 曹务春, 詹琳. 贵阳市乌当区宠物中心犬体表寄生血红扇头蜱检出劳氏立克次体[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 536-542.
[12]	赵聪, 岳治光, 宁晓冬, 菅忆晨, 黄斌斌, 卢晨阳, 张文举, 张凯慧, 菅复春, 宁长申. 一种节肢动物体外驱避试验装置的研制及初步应用[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 573-579.
[13]	郑逢云, 陈锋岭, 黄河. 15%高氯·残杀威悬浮剂等5种杀虫剂对马陆毒杀效果比较研究[J]. 中国媒介生物学及控制杂志, 2022, 33(3): 438-441.
[14]	李伊娜, 陈宇飞, 云托娅, 白潇, 刘丽飞, 郝艳阳, 吕楠男, 张胜, 李宏, 李彬, 靳木子. 内蒙古自治区中西部口岸地区2020年蜱类优势种及其病原携带情况的调查研究[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 216-220.
[15]	朱心红, 吴因平, 王金娜, 董选军, 陈莫娇, 楼挺. 浙江省义乌市2020年山羊寄生蜱监测结果分析[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 221-224.