实验室长角血蜱吸食小鼠血方法与评价

doi:10.11853/j.issn.1003.8280.2022.05.003

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (5): 627-631.DOI: 10.11853/j.issn.1003.8280.2022.05.003

实验室长角血蜱吸食小鼠血方法与评价

郑卫青, 付仁龙, 陶卉英, 刘仰青, 陈盛恩

传染病预防控制国家重点实验室研究基地, 江西省动物源与媒介生物性传染病重点实验室, 南昌市疾病预防控制中心消毒与病媒生物防制科, 江西南昌 330038

收稿日期:2022-05-20 出版日期:2022-10-20 发布日期:2022-10-14
通讯作者: 陈盛恩,E-mail:chenshengen2020@outlook.com
作者简介:郑卫青,男,博士,助理研究员,主要从事病媒生物学与病原体相互作用相关研究工作,E-mail:zhengweiqing2001@163.com
基金资助:
江西省重点研发计划一般项目(20192BBHL80013);江西省重点研发计划(20201BBG71010)

Laboratory method and evaluation of Haemaphysalis longicornis feeding on mouse blood

ZHENG Wei-qing, FU Ren-long, TAO Hui-ying, LIU Yang-qing, CHEN Sheng-en

The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, Jiangxi 330038 China

Received:2022-05-20 Online:2022-10-20 Published:2022-10-14
Supported by:
General Project of Key Research and Development Program of Jiangxi Province (No.20192BBHL80013);Key Research and Development Program of Jiangxi Province (No.20201BBG71010)

摘要/Abstract

摘要： 目的研发蜱实验室吸食小鼠血的方法。方法使用市售材料制备蜱吸血小室，评价吸食鼠血后的长角血蜱附着时长、饱血、生长和繁殖情况。结果通过蜱吸血小室供长角血蜱吸血，若蜱平均叮咬成功率为80.00%，叮咬时长1～4 d，吸血期4～6 d，平均饱血率为98.21%，蜕皮率为97.85%。成蜱饱血率为66.67%，吸血期中位数为6.5 d，饱血体质量比饥饿蜱增加了150多倍，每只雌蜱平均产卵数876粒，卵平均孵化率为78.90%，孵化期中位数为25 d。结论研制的蜱吸血小室可用于长角血蜱实验室饲养。

关键词: 蜱, 小鼠, 吸血小室, 饲养方法, 生活史

Abstract: Objective To develop the method for ticks to feed on mouse blood under laboratory conditions.Methods The blood feeding chamber for ticks was prepared with commercially available materials.The attachment,engorgement,growth,and reproduction of Haemaphysalis longicornis were evaluated after feeding on mouse blood.Results The chambers were used to feed H.longicornis.Nymphs used 1-4 d to attach themselves to mice and had a mean biting success rate of 80.00%,an engorgement duration of 4-6 d and a mean engorgement rate of 98.21%.On the average,97.85% nymphs molted to adults.Adult ticks spent 6.5 d (M) to engorge with an engorgement rate of 66.67%,and the body weight of engorged ticks increased>150-fold versus unfed ticks.Each female tick laid an average of 876 eggs.On the average,78.90% eggs hatched with the duration of 25 d (M).Conclusion The blood feeding chamber for ticks developed by the authors is suitable for rearing H.longicornis under laboratory conditions.

Key words: Tick, Mouse, Blood feeding chamber, Rearing method, Life cycle

中图分类号:

R384.4

郑卫青, 付仁龙, 陶卉英, 刘仰青, 陈盛恩. 实验室长角血蜱吸食小鼠血方法与评价[J]. 中国媒介生物学及控制杂志, 2022, 33(5): 627-631.

ZHENG Wei-qing, FU Ren-long, TAO Hui-ying, LIU Yang-qing, CHEN Sheng-en. Laboratory method and evaluation of Haemaphysalis longicornis feeding on mouse blood[J]. Chinese Journal of Vector Biology and Control, 2022, 33(5): 627-631.

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实验室长角血蜱吸食小鼠血方法与评价

Laboratory method and evaluation of Haemaphysalis longicornis feeding on mouse blood

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