福建省厦门市2020年白纹伊蚊对拟除虫菊酯类杀虫剂敏感性及击倒抗性基因研究

doi:10.11853/j.issn.1003.8280.2022.02.003

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (2): 177-182.DOI: 10.11853/j.issn.1003.8280.2022.02.003

福建省厦门市2020年白纹伊蚊对拟除虫菊酯类杀虫剂敏感性及击倒抗性基因研究

伍思翰¹, 周欣欣², 柯雪梅¹, 李玉伟³, 陈斌³, 黄婧雯³, 陈华芳¹, 林敏珍¹, 雷蕾¹, 郭志南¹, 孟凤霞²

1. 厦门市疾病预防控制中心消毒与病媒传染病预防控制科, 福建厦门 361021;
2. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 北京 102206;
3. 福建省疾病预防控制中心, 福建福州 350001

收稿日期:2021-11-10 出版日期:2022-04-20 发布日期:2022-05-09
通讯作者: 郭志南,E-mail:121644942@qq.com;孟凤霞,E-mail:mengfengxia@icdc.cn
作者简介:伍思翰,男,主管医师,主要从事病媒生物防制研究,E-mail:xmyzwsh@outlook.com;周欣欣,女,在读硕士,主要从事媒介生物抗药性研究,E-mail:zhouxinxin2021@126.com
基金资助:
福建省卫生健康科研人才培养项目-青年科研课题（2019-2-59）；厦门市医疗卫生指导性项目（3502Z20214ZD1297）；福建省科技厅自然科学基金（面上）（2020D033）；厦门市医疗卫生科技计划项目（3502Z20194096）；福建省科技计划项目引导性项目（2019D014）

Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020

WU Si-han¹, ZHOU Xin-xin², KE Xue-mei¹, LI Yu-wei³, CHEN Bin³, HUANG Jing-wen³, CHEN Hua-fang¹, LIN Min-zhen¹, LEI Lei¹, GUO Zhi-nan¹, MENG Feng-xia²

1. Department of Disinfection and Vector-borne Disease Prevention and Control, Xiamen Center for Disease Control and Prevention, Xiamen, Fujian 361021, China;
2. Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
3. Fujian Center for Disease Control and Prevention, Fuzhou, Fujian 350001, China

Received:2021-11-10 Online:2022-04-20 Published:2022-05-09
Supported by:
Health Research Talents Training Project of Fujian Province-youth Research Project (No. 2019-2-59);Xiamen City Medical and Health Guidance Project (No.3502Z20214ZD1297); Natural Science Foundation of Fujian Science and Technology Department (No. 2020D033); Xiamen Medical and Health Science and Technology Project (No. 3502Z20194096); Fujian Science and Technology Program Leading Project (No. 2019D014)

摘要/Abstract

摘要： 目的了解厦门市白纹伊蚊对拟除虫菊酯类杀虫剂的敏感性，检测击倒抗性基因（kdr）突变，为该地区白纹伊蚊防治提供科学依据。方法 2020年7月在厦门市湖里和翔安区不同方位采集白纹伊蚊幼蚊和蛹，在实验室饲养繁殖，按照世界卫生组织推荐的成蚊接触法测定其对拟除虫菊酯类杀虫剂的抗药性；提取单只白纹伊蚊基因组DNA，采用普通PCR扩增后直接测序的方法检测击倒抗性基因突变。结果厦门市湖里和翔安区白纹伊蚊接触0.03%溴氰菊酯、0.4%氯菊酯、0.08%高效氯氰菊酯、0.4%高效氯氰菊酯和0.07%高效氯氟氰菊酯24 h死亡率分别为84.33%、72.60%、26.91%、51.69%、71.68%和30.92%、53.33%、33.06%、71.53%、70.74%。除湖里区白纹伊蚊对溴氰菊酯产生可疑抗性外，其余均为抗性群体。共检测171只白纹伊蚊kdr基因，其中S989、I1011和L1014位点均未发现突变，V1016、I1532和F1534位点均存在一定程度的突变。以F1534位点突变频率最高，其次是V1016，I1532位点突变频率最低。湖里和翔安区1016G突变等位基因频率分别为13.79%和10.71%；1532T突变频率较低，翔安区为1.19%，湖里区未检测到该突变。F1534位点存在3种突变等位基因型，以1534S为主，翔安区突变频率为36.31%，湖里区为29.31%；1534C突变频率2个区差异较大，翔安区为1.79%，湖里区为25.29%；1534L突变频率最低，翔安和湖里区分别为0.60%和0.57%。结论厦门市湖里和翔安区白纹伊蚊成蚊对溴氰菊酯、氯菊酯、高效氯氰菊酯和高效氯氟氰菊酯均产生了抗性，且kdr基因也存在较高频率的突变。应加强对厦门市白纹伊蚊抗药性监测，合理使用化学杀虫剂，采取科学的综合防制措施。

关键词: 白纹伊蚊, 拟除虫菊酯类杀虫剂, 敏感性, 击倒抗性基因

Abstract: Objective To understand the sensitivity of Aedes albopictus to pyrethroid insecticides in Xiamen, China, and to detect the mutants of knockdown resistance genes (kdr), so as to provide a scientific basis for the control of Ae. albopictus in this area. Methods In July 2020, the larvae and pupae of Ae. albopictus were collected in different sampling sites in Huli district and Xiang'an district of Xiamen, and reared in the laboratory. The resistance of larvae and pupae to pyrethroid insecticides was determined according to the WHO tube test. The genomic DNA of individual Ae. albopictus was extracted and the knockdown resistance gene mutation was detected by PCR amplification followed by sequencing. Results The mosquitoes collected in Huli district and Xiang'an district of Xiamen were exposed to 0.03% deltamethrin, 0.4% permethrin, 0.08% beta-cypermethrin, 0.4% beta-cypermethrin, or 0.07% lambda-cyhalothrin for 24 h, and showed mortality rates of 84.33%, 72.60%, 26.91%, 51.69%, 71.68%, 30.92%, 53.33%, 33.06%,71.53%, and 70.74%, respectively. All the populations were resistant except that the population from Huli showed suspected resistance to deltamethrin. The kdr gene was detectable in 171 mosquitos, which showed no mutation at S989, I1011, and L1014, but had mutations at V1016, I1532, and F1534. The mutation frequency was the highest at F1534, followed by V1016, and the lowest at I1532. The allele mutation frequency of 1016G in Huli and Xiang'an was 13.79% and 10.71%, respectively. The 1532T showed low mutation frequency of 1.19% in Xiang'an and zero in Huli. There were three mutant alleles at F1534 (mainly 1534S), and the mutation frequency was 36.31% in Xiang'an and 29.31% in Huli. The mutation frequency of 1534C varied greatly between Xiang'an (1.79%) and Huli (25.29%). The mutation frequency of 1534L was the lowest, which was 0.60% in Xiang'an and 0.57% in Huli. Conclusion Adult Ae. albopictus in Huli district and Xiang'an district of Xiamen has developed resistance to deltamethrin, permethrin, beta-cypermethrin, and lambda-cyhalothrin, and shows high frequency of mutations in the kdr gene. The monitoring of drug resistance of Ae. albopictus in Xiamen should be strengthened, chemical insecticides should be used reasonably, and scientific integrated control measures should be taken.

Key words: Aedes albopictus, Pyrethroid insecticide, Susceptibility, kdr gene

中图分类号:

伍思翰, 周欣欣, 柯雪梅, 李玉伟, 陈斌, 黄婧雯, 陈华芳, 林敏珍, 雷蕾, 郭志南, 孟凤霞. 福建省厦门市2020年白纹伊蚊对拟除虫菊酯类杀虫剂敏感性及击倒抗性基因研究[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 177-182.

WU Si-han, ZHOU Xin-xin, KE Xue-mei, LI Yu-wei, CHEN Bin, HUANG Jing-wen, CHEN Hua-fang, LIN Min-zhen, LEI Lei, GUO Zhi-nan, MENG Feng-xia. Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020[J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 177-182.

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福建省厦门市2020年白纹伊蚊对拟除虫菊酯类杀虫剂敏感性及击倒抗性基因研究

Study on sensitivity and knockdown resistance genes of Aedes albopictus to pyrethroid insecticides in Xiamen, Fujian province, China, 2020

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