黑龙江省黑河市部分地区啮齿动物体表寄生恙螨调查

doi:10.11853/j.issn.1003.8280.2021.05.012

中国媒介生物学及控制杂志 ›› 2021, Vol. 32 ›› Issue (5): 570-575.DOI: 10.11853/j.issn.1003.8280.2021.05.012

黑龙江省黑河市部分地区啮齿动物体表寄生恙螨调查

母群征¹, 李欣颖², 刘蓉蓉¹, 杨军³, 栗冬梅¹, 鲁亮¹, 李贵昌¹, 侯咏⁴, 李明⁴, 宋秀平¹, 孟凤霞¹, 刘起勇¹

1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
2. 甘肃省疾病预防控制中心, 甘肃兰州 730000;
3. 逊克海关, 黑龙江黑河 164400;
4. 哈尔滨海关, 黑龙江哈尔滨 150001

收稿日期:2021-04-26 出版日期:2021-10-20 发布日期:2021-10-20
通讯作者: 李贵昌,E-mail:liguichang@icdc.cn
作者简介:母群征,男,在读硕士,主要从事病媒生物及相关传染病控制工作,E-mail:1484553691@qq.com
基金资助:
国家科技重大专项（2017ZX10303404006）

An investigation of parasitic chigger mites on the rodents in some areas of Heihe, Heilongjiang province, China

MU Qun-zheng¹, LI Xin-ying², LIU Rong-rong¹, YANG Jun³, LI Dong-mei¹, LU Liang¹, LI Gui-chang¹, HOU Yong⁴, LI Ming⁴, SONG Xiu-ping¹, MENG Feng-xia¹, LIU Qi-yong¹

1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
2. Gansu Provincial Center for Disease Control and Prevention, Lanzhou, Gansu 730000, China;
3. Xunke Customs House, Heihe, Heilongjiang 164400, China;
4. Harbin Customs District, Harbin, Heilongjiang 150001, China

Received:2021-04-26 Online:2021-10-20 Published:2021-10-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404006)

摘要/Abstract

摘要： 目的调查了解黑龙江省黑河市部分地区啮齿动物体表寄生恙螨的种类组成及分布。方法 2015年9月中旬在黑河市瑷珲区、逊克县、五大连池市采用鼠夹法捕获啮齿动物，捡取体表寄生恙螨；依据形态特征鉴定恙螨种类，通过形态特征和DNA条形码技术鉴定鼠和鼠兔种类；计算各地区、生境的啮齿动物种类构成、带恙螨率、恙螨指数并进行统计分析；通过巢式PCR检测鼠恙虫病东方体病原核酸。结果本次共捕获啮齿动物170只，包括2目3科6属8种。逊克县的火山地质公园以红背䶄（38.26%）、满洲里鼠兔（34.78%）和棕背䶄（14.78%）为主，五大连池市和瑷珲区农田周边以黑线姬鼠为主，分别占76.67%和64.00%。啮齿动物恙虫病东方体检测均为阴性。五大连池市和瑷珲区鼠体均未捡到恙螨，逊克啮齿动物检获11 256只恙螨，包括2属5种，高丽新恙螨为优势螨种（87.50%）。满洲里鼠兔、红背䶄和棕背䶄是恙螨的主要寄生宿主。结论黑河市不同调查地点啮齿动物种群构成及不同鼠种携带恙螨数量存在明显差异。

关键词: 啮齿动物, 恙螨, 满洲里鼠兔, 恙虫病东方体

Abstract: Objective To investigate the species composition and distribution of ectoparasitic chigger mites on the rodents in some areas of Heihe, Heilongjiang province, China. Methods Rodents were captured using rat snap traps in Aihui district, Xunke county, and Wudalianchi of Heihe in mid-September, 2015. Chigger mites were collected from the body surface of rodents. The species of chigger mites was identified according to their morphologic characteristics, and the species of rodents and pikas were identified by morphology and DNA barcoding technique. The species composition, chigger infestation rate, chigger index in each area and habitat were calculated and analyzed. The nucleic acid of Orientia tsutsugamushi was detected by nested PCR. Results A total of 170 rodents were captured, involving 8 species of 6 genera from 3 families under 2 orders. Myodes rutilus (38.26%), Ochotona mantchurica (34.78%), and M. rufocanus (14.78%) were the dominant species in the volcanic geopark of Xunke county. Apodemus agrarius was the dominant species in the farmland of Wudalianchi (76.67%) and Aihui district (64.00%). Or. tsutsugamushi was negative in all rodents captured. No chigger mite was found on the bodies of rodents from Wudalianchi and Aihui district, while 11 256 chigger mites were found on rodents from Xunke county, belonging to 5 species of 2 genera, with the dominant mite species Neotrombicula gardellai (87.50%). The main hosts of chigger mites were O. mantchurica, M. rutilus, and M. rufocanus. Conclusion The different areas of Heihe have distinct species compositions of rodents, and different species of rodents carry distinct numbers of chigger mite.

Key words: Rodent, Chigger mite, Ochotona mantchurica, Orientia tsutsugamushi

中图分类号:

母群征, 李欣颖, 刘蓉蓉, 杨军, 栗冬梅, 鲁亮, 李贵昌, 侯咏, 李明, 宋秀平, 孟凤霞, 刘起勇. 黑龙江省黑河市部分地区啮齿动物体表寄生恙螨调查[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 570-575.

MU Qun-zheng, LI Xin-ying, LIU Rong-rong, YANG Jun, LI Dong-mei, LU Liang, LI Gui-chang, HOU Yong, LI Ming, SONG Xiu-ping, MENG Feng-xia, LIU Qi-yong. An investigation of parasitic chigger mites on the rodents in some areas of Heihe, Heilongjiang province, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(5): 570-575.

参考文献

[1] 李明,曹晓梅,张晓龙,等. 2006-2009年黑龙江省中俄双边口岸鼠类调查名录[J]. 中国媒介生物学及控制杂志,2011,22(1):55-56.Li M,Cao XM,Zhang XL,et al. List of rodents at bilateral ports along the China-Russia border in Heilongjiang province from 2006 to 2009[J]. Chin J Vector Biol Control,2011,22(1):55-56.
[2] 任清明,刘国平,杨国平,等. 黑龙江省中俄边境地区鼠类的调查研究[J]. 中国媒介生物学及控制杂志,2009,20(3):219-220.Ren QM,Liu GP,Yang GP,et al. Investigation of rodents from China-Russia frontier of Heilongjiang province[J]. Chin J Vector Biol Control,2009,20(3):219-220.
[3] 张晓龙,赵婷婷,魏莲,等. 2009年中俄边境黑龙江四口岸啮齿动物及其感染病原调查研究[J]. 中国国境卫生检疫杂志,2010,33(6):387-390. DOI:10.16408/j.1004-9770.2010.06.006.Zhang XL,Zhao TT,Wei L,et al. The ectoparasite and possible pathogens of rodent infections at four ports in Heilongjiang province along the Sino-Russia boundary[J]. Chin J Front Health Quarantine,2010,33(6):387-390. DOI:10.16408/j.1004-9770.2010.06.006.
[4] 杨军,关承春,温占清,等. 逊克口岸鼠类本底调查报告[J]. 中国国境卫生检疫杂志,2006,29(6):350-351. DOI:10.3969/j.issn.1004-9770.2006.06.012.Yang J,Guan CC,Wen ZQ,et al. Investigation on background of rat at Xunke port[J]. Chin J Frontier Health Quarantine,2006,29(6):350-351. DOI:10.3969/j.issn.1004-9770.2006.06.012.
[5] 王亚玉,刘国平,李东力,等. 东北中朝俄边境地区鼠体外寄生虫种类组成研究[J]. 中国媒介生物学及控制杂志,2005,16(1):44-46. DOI:10.3969/j.issn.1003-4692.2005.01.015.Wang YY,Liu GP,Li DL,et al. Study of species composition on the ectoparasites of the rodent in frontiers of Korea,Russia and northeast China[J]. Chin J Vector Biol Control,2005,16(1):44-46. DOI:10.3969/j.issn.1003-4692.2005.01.015.
[6] 曹晓梅,胡孔新,王静,等. 中俄双边口岸鼠类及体表寄生虫调查[J]. 寄生虫与医学昆虫学报,2011,18(4):230-235. DOI:10.3969/j.issn.1005-0507.2011.04.007.Cao XM,Hu KX,Wang J,et al. A survey of rodent and rodent-borne ectozoa in ports between China and Russia[J]. Acta Parasitol Med Entomol Sin,2011,18(4):230-235. DOI:10.3969/j.issn.1005-0507.2011.04.007.
[7] 张晓龙,赵臻君,李明,等. 中俄边境中部地区不同生境鼠类初步调查研究[J]. 中华卫生杀虫药械,2011,17(1):34-36. DOI:10.19821/j.1671-2781.2011.01.011.Zhang XL,Zhao ZJ,Li M,et al. Survey of rodent diversity in different habitats in the central border area of China and Russia[J]. Chin J Hyg Insect Equip,2011,17(1):34-36. DOI:10.19821/j.1671-2781.2011.01.011.
[8] Luce-Fedrow A,Lehman ML,Kelly DJ,et al. A review of scrub typhus (Orientia tsutsugamushi and related organisms):then,now,and tomorrow[J]. Trop Med Infect Dis,2018,3(1):8. DOI:10.3390/tropicalmed3010008.
[9] 李贵昌,刘起勇. 恙虫病的流行现状[J]. 疾病监测,2018,33(2):129-138. DOI:10.3784/j.issn.1003-9961.2018.02.006.Li GC,Liu QY. Epidemic trend of scrub typhus in the world[J]. Dis Surveill,2018,33(2):129-138. DOI:10.3784/j.issn.1003-9961.2018.02.006.
[10] 李贵昌,栗冬梅,李焱,等. 2006-2016年中国恙虫病流行特征分析[J]. 疾病监测,2018,33(2):139-143. DOI:10.3784/j.issn.1003-9961.2018.02.007.Li GC,Li DM,Li Y,et al. Epidemiology of scrub typhus in China,2006-2016[J]. Dis Surveill,2018,33(2):139-143. DOI:10.3784/j.issn.1003-9961.2018.02.007.
[11] Weitzel T,Makepeace BL,Elliott I,et al. Marginalized mites:neglected vectors of neglected diseases[J]. PLoS Negl Trop Dis,2020,14(7):e0008297. DOI:10.1371/journal.pntd.0008297.
[12] 郑智民,姜志宽,陈安国. 啮齿动物学[M]. 2版. 上海:上海交通大学出版社,2012:33-142.Zheng ZM,Jiang ZK,Chen AG. Rodent biology[M]. 2nd ed. Shanghai:Shanghai Jiaotong University Press,2012:33-142.
[13] Stekolnikov AA. Leptotrombidium (Acari:Trombiculidae) of the world[J]. Zootaxa,2013,3728(1):1-173. DOI:10.11646/zootaxa.3728.1.1.
[14] Lissovsky AA,Yatsentyuk SP,Ge DY. Phylogeny and taxonomic reassessment of pikas Ochotona pallasii and O. argentata (Mammalia,Lagomorpha)[J]. Zoologica Scripta,2016,45(6):583-594. DOI:10.1111/zsc.12180.
[15] Wang XY,Liang D,Jin W,et al. Out of Tibet:genomic perspectives on the evolutionary history of extant pikas[J]. Mol Biol Evol,2020,37(6):1577-1592. DOI:10.1093/molbev/msaa026.
[16] 杨军,鞠文东,焦丹,等. 黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查[J]. 中国媒介生物学及控制杂志,2015,26(3):238-241. DOI:10.11853/j.issn.1003.4692.2015.03.005.Yang J,Ju WD,Jiao D,et al. Investigation on rodent-borne pathogens in volcano lava areas of Xunke county,Heilongjiang China[J]. Chin J Vector Biol Control,2015,26(3):238-241. DOI:10.11853/j.issn.1003.4692.2015.03.005.
[17] 冯祚建,郑昌琳. 中国鼠兔属(Ochotona)的研究:分类与分布[J]. 兽类学报,1985,5(4):269-289. DOI:10.16829/j.slxb. 1985.04.006.Feng ZJ,Zheng CL. Studies on the pikas (genus:Ochotona) of China-taxonomic notes and distribution[J]. Acta Theriol Sin,1985,5(4):269-289. DOI:10.16829/j.slxb.1985.04.006.
[18] Lissovsky AA. A new subspecies of Manchurian pika Ochotona mantchurica (Lagomorpha,Ochotonidae) from the Lesser Khinggan Range,China[J]. Russian J Theriol,2015,14(2):145-152. DOI:10.15298/rusjtheriol.14.2.03.
[19] Lissovsky AA. Taxonomic revision of pikas Ochotona (Lagomorpha,Mammalia) at the species level[J]. Mammalia,2014,78(2):199-216. DOI:10.1515/mammalia-2012-0134.
[20] 温廷桓. 中国沙螨[恙螨] [M]. 上海:学林出版社,1984:62,167,170.Wen TH. Sand mites of China[M]. Shanghai:Xue Lin Publishing House,1984:62,167,170.
[21] 刘国平,李东力,陈春田,等. 我国东北三省的恙螨[J]. 中国媒介生物学及控制杂志,2003,14(6):444-446. DOI:10.3969/j.issn.1003-4692.2003.06.018.Liu GP,Li DL,Chen CT,et al. Chigger mites from three provinces of northeast China[J]. Chin J Vector Biol Control,2003,14(6):444-446. DOI:10.3969/j.issn.1003-4692.2003. 06.018.
[22] 刘国平,鲁志新. 我国东北地区的恙螨和恙虫病[J]. 中国公共卫生,2000,16(9):779-781. DOI:10.3321/j.issn:1001-0580. 2000.09.004.Liu GP,Lu ZX. Chigger mites and scrub typhus in northeast China[J]. China Public Health,2000,16(9):779-781. DOI:10.3321/j.issn:1001-0580.2000.09.004.
[23] Vercammen-Grandjean PH,Langston RL. The chigger mites of the world (Acarina:Trombiculidae & Leeuwenhoekiidae). III Leptotrombidium complex[M]. San Francisco:George Williams Hooper Foundation,University of California,1976:290-292.
[24] 鲁志新,温青莉,解志刚,等. 东北三省部分地区人血清恙虫病立克次体抗体调查[J]. 中国人兽共患病杂志,1999,15(4):106. DOI:10.3969/j.issn.1002-2694.1999.04.033.Lu ZX,Wen QL,Xie ZG,et al. Investigation on Rickttsia tsutsugamushi antibodies of human serum from northeast of China[J]. Chin J Zoonoses,1999,15(4):106. DOI:10.3969/j.issn.1002-2694.1999.04.033.
[25] 刘国平,陶增珖,全理华,等. 我国东北边境地区恙螨区系和生态调查[J]. 生态学杂志,1988,7(2):13-16.Liu GP,Tao ZG,Quan LH,et al. Faunal and ecological investigations of chigger mites in frontiers of northeast China[J]. J Ecol,1988,7(2):13-16.
[26] 李贵昌,李欣颖,刘晶,等. 黑龙江省庆安县小兽体表恙螨及恙虫病东方体感染调查[J]. 中国媒介生物学及控制杂志,2017,28(1):1-3,15. DOI:10.11853/j.issn.1003.8280.2017. 01.001.Li GC,Li XY,Liu J,et al. Investigation of chigger mites and infection of Orientia tsutsugamushi in small mammals in Qing'an county[J]. Chin J Vector Biol Control,2017,28(1):1-3,15. DOI:10.11853/j.issn.1003.8280.2017.01.001.

黑龙江省黑河市部分地区啮齿动物体表寄生恙螨调查

An investigation of parasitic chigger mites on the rodents in some areas of Heihe, Heilongjiang province, China

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