海南省白纹伊蚊中肠感染沃尔巴克氏体种群结构调查研究

doi:10.11853/j.issn.1003.8280.2021.03.019

中国媒介生物学及控制杂志 ›› 2021, Vol. 32 ›› Issue (3): 355-360.DOI: 10.11853/j.issn.1003.8280.2021.03.019

海南省白纹伊蚊中肠感染沃尔巴克氏体种群结构调查研究

刘彪^1,2, 康迅^1,2, 刘琳^1,2, 赵秀娟^1,2, 符玉梅^1,2, 田国振^1,2, 康乐³, 夏乾峰^1,2

1. 海南医学院热带医学与检验医学院, 海南海口 571199;
2. 海南医学院热带转化医学教育部重点实验室, 海南海口 571199;
3. 中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101

收稿日期:2020-11-15 出版日期:2021-06-20 发布日期:2021-06-20
通讯作者: 康乐,E-mail:lkang@ioz.ac.cn;夏乾峰,E-mail:xiaqianfeng@hainmc.edu.cn
作者简介:刘彪,男,在读硕士,医师,主要从事病媒生物检测与防治工作,E-mail:luibiao198876@qq.com;康迅,女,硕士,实验师,主要从事病媒生物检测与防治工作,E-mail:kangxun2017@163.com
基金资助:
国家科技重大专项（2018ZX10101003-001-009）

An investigation of population structure in the Aedes albopictus midgut infected with Wolbachia in Hainan province, China

LIU Biao^1,2, KANG Xun^1,2, LIU Lin^1,2, ZHAO Xiu-juan^1,2, FU Yu-mei^1,2, TIAN Guo-zhen^1,2, KANG Le³, XIA Qian-feng^1,2

1. Laboratory of Tropical Biomedicine and Biotechnology, Hainan Medical University, Haikou, Hainan 571199, China;
2. Key Laboratory of Tropical Translalional Medicine of Ministry of Education and School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, China;
3. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Received:2020-11-15 Online:2021-06-20 Published:2021-06-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2018ZX10101003-001-009)

摘要/Abstract

摘要： 目的了解海南省白纹伊蚊种群中沃尔巴克氏体（Wolbachia）的感染情况、丰度差异性及遗传亲缘关系，为蚊媒传染病的生物防制提供依据。方法 2018年7－9月，选取海南省陵水、定安、海口、文昌、三亚、乐东、屯昌和五指山8个县（市），在野外采集成蚊，应用形态学及细胞色素C氧化酶亚基Ⅰ基因对蚊种进行鉴定，通过16S rRNA的V3-V4高变区对中肠微生物菌种鉴定检测，利用χ²检验分析沃尔巴克氏体在不同地区白纹伊蚊中肠微生物种群结构的丰度差异是否有统计学意义，采用MEGA 7.0软件对所获基因序列进行遗传距离分析。结果海南省的白纹伊蚊中肠内，沃尔巴克氏体的感染占比以定安县、三亚市、陵水县和海口市较高，分别占84.67%、80.34%、68.77%和56.89%，为优势菌种，感染率极低的有乐东县和五指山市，分别占1.50%和0.01%。不同地区沃尔巴克氏体的丰度在肠道微生物构成中差异有统计学意义（χ²=322.950，P<0.001），遗传距离分析提示不同地区存在菌株的交换，同一地区基因也存在一定的差异。结论海南省的白纹伊蚊中肠沃尔巴克氏体感染率高，但在乐东和五指山地区感染率低，各地区的白纹伊蚊中肠感染沃尔巴克氏体的丰度存在一定的差异性，各地区菌株存在遗传多样性。

关键词: 白纹伊蚊, 中肠微生物, 16S rRNA, 沃尔巴克氏体, 海南省

Abstract: Objective To investigate the prevalence, abundance difference, and genetic relationship of Wolbachia in Aedes albopictus population in Hainan province, China, and to provide a basis for the biological control of mosquito-borne infectious diseases. Methods From July to September 2018, eight counties/cities in Hainan province, Lingshui, Ding'an, Haikou, Wenchang, Sanya, Ledong, Tunchang, and Wuzhishan, were selected in this study. After field collection of adult mosquitoes, morphology and cytochrome c oxidase subunit Ⅰ (COⅠ) gene were used to identify the mosquito species; the 16S rRNA V3-V4 hypervariable regions were used to identify the microbial species in their midgut; the chi-square test was used to analyze whether there was a significant difference in Wolbachia abundance in the midgut microbiota of Ae. albopictus between different areas. MEGA 7.0 software was used to analyze the genetic distances between the obtained gene sequences. Results Among the microorganisms in the midgut of Ae. albopictus in Hainan province, Wolbachia accounted for a relatively high proportion in Ding'an county (84.67%), Sanya city (80.34%), Lingshui county (68.77%), and Haikou city (56.89%), and was the dominant species; the infection rates in Ledong county (1.50%) and Wuzhishan city (0.01%) were extremely low. The abundance of Wolbachia in the midgut microbiota was significantly different between different areas (χ²=322.950, P<0.001). The genetic distance analysis suggested that there were exchanges of Wolbachia strains between different areas, and there were some differences in genes between the Wolbachia strains in the same region. Conclusion The midgut of Ae. albopictus has a high infection rate with Wolbachia in Hainan province, but there are low infection rates in Ledong county and Wuzhishan city; there are certain differences in the abundance of Wolbachia in the midgut of Ae. albopictus between different areas; there is genetic diversity among the Wolbachia strains in various areas.

Key words: Aedes albopictus, Midgut microbiota, 16S rRNA, Wolbachia, Hainan province

中图分类号:

刘彪, 康迅, 刘琳, 赵秀娟, 符玉梅, 田国振, 康乐, 夏乾峰. 海南省白纹伊蚊中肠感染沃尔巴克氏体种群结构调查研究[J]. 中国媒介生物学及控制杂志, 2021, 32(3): 355-360.

LIU Biao, KANG Xun, LIU Lin, ZHAO Xiu-juan, FU Yu-mei, TIAN Guo-zhen, KANG Le, XIA Qian-feng. An investigation of population structure in the Aedes albopictus midgut infected with Wolbachia in Hainan province, China[J]. Chines Journal of Vector Biology and Control, 2021, 32(3): 355-360.

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海南省白纹伊蚊中肠感染沃尔巴克氏体种群结构调查研究

An investigation of population structure in the Aedes albopictus midgut infected with Wolbachia in Hainan province, China

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