抗凝血杀鼠剂选择压力下黄毛鼠抗药性相关解毒酶的研究

doi:10.11853/j.issn.1003.8280.2020.06.006

摘要/Abstract

摘要： 目的探讨抗凝血杀鼠剂选择压力下黄毛鼠抗药性与代谢解毒酶的关系，为阐明黄毛鼠抗药性机制奠定基础。方法采用酶标仪分别检测黄毛鼠血清和肝脏中相关解毒酶细胞色素P450（CYP450）、NAD（P）H醌氧化还原酶1（NQO1）、细胞色素b5（Cytb5）的含量和活性，并进行不同类群间的比对分析，分析这种差异和抗药性水平的相互关系。结果在广东省江门市黄毛鼠种群中，无论是敏感鼠还是抗药性鼠，肝脏中解毒酶的含量及活性均高于血清中的含量和活性，其中肝脏中NQO1的含量和活性、Cytb5的含量在敏感鼠和抗药性鼠之间差异均有统计学意义（t₁=2.408，P₁=0.037；t₂=2.515，P₂=0.031；t₃=2.281，P₃=0.046），且敏感鼠高于抗药性鼠，而血清中3种酶的含量和活性、肝脏中CYP450的含量和活性以及Cytb5的活性差异均无统计学意义（均P>0.05）。结论 3种酶中仅肝脏NQO1的含量和活性、Cytb5的含量与黄毛鼠的抗药性相关，推测鼠类对抗凝血类杀鼠剂的抗药性是一个多因素协同作用的结果，应将多个因素结合在一起进行综合分析，才能更好地阐明鼠类对抗凝血剂的抗药性作用机制。

关键词: 黄毛鼠, 抗药性, 细胞色素P450, NAD（P）H醌氧化还原酶1, 细胞色素b5

Abstract: Objective To explore the relationship between the resistance of Rattus losea and its metabolic detoxifying enzymes under the selective pressure of anticoagulant rodenticides, and to lay a foundation for elucidating the resistance mechanism. Methods A microplate reader was used to determine the contents and activities of cytochrome P450 (CYP450), NAD(P)H quinone oxidoreductase 1 (NQO1), and cytochrome b5 (Cytb5) in serum and liver of R. losea; meanwhile, comparative analyses were performed between different populations for the relationship of the difference with resistance level. Results The content and activity of detoxifying enzymes in liver were higher than those in serum in both sensitive and resistant R. losea populations in Jiangmen, Guangdong province, China. The content and activity of NQO1 and the content of Cytb5 in liver were significantly higher in the sensitive rodents than in the resistant rodents (t₁=2.408, P₁=0.037; t₂=2.515, P₂=0.031; t₃=2.281, P₃=0.046). However, there were no significant differences in the contents and activities of the three enzymes in serum, the content and activity of CYP450 in liver and the activity of Cytb5 (all P>0.05). Conclusion Among the three enzymes, only the content and activity of NQO1 and the content of Cytb5 in the liver are related to the insecticide resistance of R. losea. It is speculated that the resistance of rodents to anticoagulant rodenticides is a synergistic effect of multiple factors. Therefore, a comprehensive analysis of multiple factors should be performed to better clarify the mechanism of action of rodents' resistance to anticoagulant rodenticides.

Key words: Rattus losea, Insecticide resistance, Cytochrome P450, NAD(P)H quinone oxidoreductase 1, Cytochrome b5

中图分类号:

姚丹丹, 姜洪雪, 隋晶晶, 冯志勇. 抗凝血杀鼠剂选择压力下黄毛鼠抗药性相关解毒酶的研究[J]. 中国媒介生物学及控制杂志, 2020, 31(6): 657-661.

YAO Dan-dan, JIANG Hong-xue, SUI Jing-jing, FENG Zhi-yong. A study on detoxifying enzymes related to the resistance of Rattus losea to anticoagulant rodenticides under its selective pressure[J]. Chines Journal of Vector Biology and Control, 2020, 31(6): 657-661.

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