上海市虹口区2015-2019年重要病媒生物对常用杀虫剂的抗药性调查

doi:10.11853/j.issn.1003.8280.2020.02.005

摘要/Abstract

摘要： 目的了解上海市虹口区白纹伊蚊、家蝇及德国小蠊对常用杀虫剂的抗药性水平，为杀虫剂的合理选择和使用提供科学依据。方法白纹伊蚊幼蚊抗性测定采用世界卫生组织推荐的幼虫浸渍法，测定杀虫剂对幼蚊的半数致死浓度（LC₅₀）。家蝇抗性测定方法采用微量点滴法，测定杀虫剂对家蝇半数致死剂量（LD₅₀）。德国小蠊抗性测定采用药膜法，测定杀虫剂对德国小蠊的半数击倒时间（KT₅₀）。结果 2017和2019年上海市虹口区白纹伊蚊幼蚊对双硫磷的的抗性倍数分别为0.44和4.94倍，表现为敏感和低抗；2017年对高效氯氰菊酯的抗性倍数为16.89倍，表现为中抗；对溴氰菊酯的抗性倍数为34.25倍，表现为中抗；对残杀威的抗性倍数为1.59倍，表现为敏感；2019年对氯菊酯的抗性倍数为13.40倍，表现为中抗。2016和2018年家蝇对高效氯氰菊酯的抗性倍数为29.07和39.53倍，表现为高抗；2016年对溴氰菊酯和敌敌畏的抗性倍数为112.86和4.37倍，表现为高抗和低抗；2018年对甲基嘧啶磷和氯菊酯的抗性倍数为4.03和13.08倍，表现为低抗和中抗。2015和2018年德国小蠊对高效氯氰菊酯的抗性倍数为3.05和1.60倍，表现为低抗和敏感；对溴氰菊酯的抗性倍数为4.31和6.13倍，表现为低抗；对乙酰甲胺磷的抗性倍数为0.93和1.10倍，表现为敏感；2015年对残杀威的抗性倍数为2.69倍，表现为低抗。结论虹口区白纹伊蚊幼蚊、家蝇和德国小蠊对常用杀虫剂已表现出不同程度抗性，应加强对杀虫剂抗药性的监测，制定科学、合理的杀虫剂用药策略。

关键词: 白纹伊蚊, 家蝇, 德国小蠊, 杀虫剂, 抗药性

Abstract: Objective To investigate the resistance to common insecticides in Aedes albopictus, Musca domestica, and Blattella germanica in Hongkou district, Shanghai, China, and to provide a scientific basis for rational selection and use of insecticides. Methods The larval dipping method recommended by WHO was used to test the resistance of Ae. albopictus larvae to insecticides by measuring median lethal concentrations. Micro-topical application was used to test the resistance of M. domestica to insecticides by determining median lethal doses. The residual film method was applied to test the resistance of B. germanica to insecticides by recording median knockdown time. Results In 2017 and 2019, Ae. albopictus larvae in Hongkou district of Shanghai showed resistance ratios of 0.44 (low resistance) and 4.94 (moderate resistance) to temephos, respectively; in 2017, the resistance ratio to beta-cypermethrin was 16.89 (moderate resistance); deltamethrin resistance ratios were 34.25 (moderate resistance); the resistance ratio to propoxur was 1.59 (sensitive); in 2019, the larvae exhibited a moderate resistance level of 13.40 to permethrin. In 2016 and 2018, M. domestica were at high resistance levels of 29.07 and 39.53 to beta-cypermethrin, respectively; in 2016, the ratios for deltamethrin and dichlorvos were 112.86 (high resistance) and 4.37 (low resistance), respectively; in 2018, the ratios for pirimiphos-methyl and permethrin were 4.03 (low resistance) and 13.08 (moderate resistance), respectively. In 2015 and 2018, the resistance ratios of B. germanica to beta-cypermethrin were 3.05 (low resistance) and 1.60 (sensitive), respectively; the ratios for deltamethrin were 4.31 (low resistance) and 6.13 (low resistance), respectively; the ratios for acephate were 0.93 (low resistance) and 1.10 (low resistance), respectively; in 2015, B. germanica showed a low resistance ratio of 2.69 to propoxur. Conclusion Aedes albopictus, M. domestica, and B. germanica in Hongkou district have varying degrees of resistance to commonly used insecticides. It is necessary to strengthen insecticide resistance surveillance and formulate management strategies for scientific and rational use of insecticides.

Key words: Aedes albopictus, Musca domestica, Blattella germanica, Insecticide, Insecticide resistance

中图分类号:

王飞, 蒋璐, 黄慧嫦, 刘洪霞, 陈道湧. 上海市虹口区2015-2019年重要病媒生物对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 143-147.

WANG Fei, JIANG Lu, HUANG Hui-chang, LIU Hong-xia, CHEN Dao-yong. Resistance of main vectors to commonly used insecticides in Hongkou district, Shanghai, China, 2015-2019[J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 143-147.

参考文献

[1] 冷培恩. 重视病媒生物控制切断疾病传播途径[J]. 上海预防医学, 2019, 31(8):615-616. DOI:10.19428/j.cnki.sjpm.2019. 19704. Leng PE. Pay attention to vector biological control and cut off the transmission way of disease[J]. Shanghai J Prevent Med,2019,31(8):615-616. DOI:10.19428/j.cnki.sjpm.2019.19704.
[2] 国家卫生健康委员会. 全国健康城市评价指标体系(2018版)政策解读[J]. 医学信息学杂志,2018(4):94. National Health Commission of the People's Republic of China. Policy interpretation of national health city evaluation index system (2018 edition)[J]. J Med Informatics,2018(4):94.
[3] 任志华,潘引君,刘天,等. 上海市青浦区卫生杀虫剂使用状况及蚊蝇抗药性研究[J]. 上海预防医学,2019,31(8):632-636. DOI:10.19428/j.cnki.sjpm.2019.19529. Ren ZH,Pan YJ,Liu T,et al. Application of sanitary insecticides and drug resistance of Aedes albopictus L. and Musca domestica L. in Qingpu district of Shanghai[J]. Shanghai J Prevent Med,2019,31(8):632-636. DOI:10.19428/j.cnki.sjpm.2019.19529.
[4] 吕锡宏,刘洪霞,郭晓芹,等. 上海市松江区蚊虫对不同杀虫剂的抗性分析[J]. 上海预防医学,2015,27(6):315-317. DOI:10.19428/j.cnki.sjpm.2015.06.004. Lyu XH,Liu HX,Guo XQ,et al. Mosquito resistance against different insecticides in Songjiang district of Shanghai[J]. Shanghai J Prevent Med,2015,27(6):315-317. DOI:10.19428/j.cnki.sjpm.2015.06.004.
[5] 刘洪霞,冷培恩,徐仁权,等. 上海地区蚊虫对常用杀虫剂的抗性及防治对策[J]. 中华卫生杀虫药械,2009,15(2):112-115. DOI:10.19821/j.1671-2781.2009.02.011. Liu HX,Leng PE,Xu RQ,et al. Study on mosquito resistance to common used insecticides and its control measure in Shanghai[J]. Chin J Hyg Insect Equip,2009,15(2):112-115. DOI:10.19821/j.1671-2781.2009.02.011.
[6] 王飞,蒋璐,陆珏磊,等. 上海市虹口区白纹伊蚊幼虫对常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械,2016,21(5):513-514. DOI:10.19821/j.1671-2781.2016.05.031. Wang F,Jiang L,Lu JL,et al. Investigation of Aedes albopictus resistantce to commonly used insecticides in Hongkou[J]. Chin J Hyg Insect Equip,2016,21(5):513-514. DOI:10.19821/j.1671-2781.2016.05.031.
[7] 王飞,蒋璐,阮玉琦. 上海市虹口区家蝇对常用杀虫剂的抗药性测定[J]. 上海预防医学,2019,31(8):637-639. DOI:10.19428/j.cnki.sjpm.2019.19530. Wang F,Jiang L,Ruan YQ. Resistance of Musca domestica L. to commonly used insecticides measured in Hongkou district of Shanghai[J]. Shanghai J Prevent Med,2019,31(8):637-639. DOI:10.19428/j.cnki.sjpm.2019.19530.
[8] 蒋璐,王飞,王丹华,等. 上海市虹口区德国小蠊对5种杀虫剂的抗性调查[J]. 中华卫生杀虫药械,2016,22(6):622-623. DOI:10.19821/j.1671-2781.2016.06.032. Jiang L,Wang F,Wang DH,et al. Resistance of Blattella germanica to five insecticides in Hongkou district of Shanghai[J]. Chin J Hyg Insect Equip,2016,22(6):622-623. DOI:10.19821/j.1671-2781.2016.06.032.
[9] 王义冠,师灿南,林国松,等. 广东省潮州市白纹伊蚊对常用杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志,2016,27(3):228-231. DOI:10.11853/j.issn.1003.8280.2016.03.004. Wang YG,Shi CN,Lin GS,et al. Resistance of Aedes albopictus in Chaozhou city,China to commonly used insecticides[J]. Chin J Vector Biol Control,2016,27(3):228-231. DOI:10.11853/j.issn.1003.8280.2016.03.004.
[10] 亓云鹏,富小飞,查亦薇,等. 浙江省嘉兴市主要病媒生物对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2019,30(3):337-340. DOI:10.11853/j.issn.1003.8280. 2019.03.026. Qi YP,Fu XF,Zha YW,et al. Study on resistance of main vectors to commonly used insecticides in Jiaxing,China[J]. Chin J Vector Biol Control,2019,30(3):337-340. DOI:10.11853/j.issn.1003.8280.2019.03.026.
[11] 陆宝麟. 媒介蚊虫化学防治的思考[J]. 中华卫生杀虫药械,2002,8(1):3-5. Lu BL. Discussion on the chemical control of mosquitoes[J]. Chin J Hyg Insect Equip,2002,8(1):3-5.
[12] 冷培恩,高强,刘洪霞. 公共环境治理与蚊媒控制[J]. 上海预防医学,2016,28(10):687-692. DOI:10.19428/j.cnki.sjpm.2016. 10.005. Leng PE,Gao Q,Liu HX. Environmental management and mosquito control[J]. Shanghai J Prevent Med,2016,28(10):687-692. DOI:10.19428/j.cnki.sjpm.2016.10.005.
[13] 冷培恩,刘洪霞,吴寰宇,等. 上海市病媒生物控制30年回顾[J]. 上海预防医学,2019,31(2):125-133. DOI:10.19428/j.cnki.sjpm.2019.18999. Leng PE,Liu HX,Wu HY,et al. Thirty-year review of vector control in Shanghai[J]. Shanghai J Prevent Med,2019,31(2):125-133. DOI:10.19428/j.cnki.sjpm.2019.18999.
[14] 刘洪霞,朱江,刘曜,等. 上海地区2015-2016年白纹伊蚊幼虫监测及抗药性调查[J]. 中国媒介生物学及控制杂志,2017,28(4):305-307. DOI:10.11853/j.issn.1003.8280.2017.04.001. Liu HX,Zhu J,Liu Y,et al. Study on the seasonal dynamics and insecticides resistance of Aedes albopictus larvae,in Shanghai,2015-2016[J]. Chin J Vector Biol Control,2017,28(4):305-307. DOI:10.11853/j.issn.1003.8280.2017.04.001.
[15] 王飞,高强,吕锡宏,等. 上海市地面排水系统2017年蚊虫孳生状况调查研究[J]. 中国媒介生物学及控制杂志,2018,29(3):259-262. DOI:10.11853/j.issn.1003.8280.2018.03.010. Wang F,Gao Q,Lyu XH,et al. Breeding status of mosquitoes in municipal drainage system of Shanghai,China in 2017[J]. Chin J Vector Biol Control,2018,29(3):259-262. DOI:10.11853/j.issn.1003.8280.2018.03.010.
[16] 冷培恩,周毅彬,徐劲秋. 上海地区蝇类抗药性现状及防治对策[J]. 中华卫生杀虫药械,2004,10(3):142-147. Leng PE,Zhou YB,Xu JQ. Resistance and control countermeasures of flies in Shanghai[J]. Chin J Hyg Insect Equip,2004,10(3):142-147.
[17] 吴红照,刘营,黄文忠,等. 浙江省浦江县"无蚊村"建设的效果评价[J]. 中国媒介生物学及控制杂志,2018,29(3):283-286. DOI:10.11853/j.issn.1003.8280.2018.03.016. Wu HZ,Liu Y,Huang WZ,et al. Evaluation on construction of "mosquito-free village" in Pujiang county,Zhejiang,China[J]. Chin J Vector Biol Control,2018,29(3):283-286. DOI:10.11853/j.issn.1003.8280.2018.03.016.
[18] 冷培恩,刘洪霞,徐劲秋,等. 有害生物防治的量化控虫技术[J]. 中华卫生杀虫药械,2016,21(3):223-225. DOI:10. 19821/j.1671-2781.2015.03.002. Leng PE,Liu HX,Xu JQ,et al. Quantitative pest control technology in pest control operation[J]. Chin J Hyg Insect Equip,2016,21(3):223-225. DOI:10.19821/j.1671-2781. 2015.03.002.