我国不同地理种群白纹伊蚊乙酰胆碱酯酶基因分布调查

doi:10.11853/j.issn.1003.8280.2020.02.003

中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (2): 133-136.DOI: 10.11853/j.issn.1003.8280.2020.02.003

所属专题：抗药性监测专题

我国不同地理种群白纹伊蚊乙酰胆碱酯酶基因分布调查

朱彩英¹, 赵春春¹, 刘起勇¹, 李贵昌¹, 闫冬明^1,2, 宋秀平¹, 王君¹, 孟凤霞¹

1 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
2山东第一医科大学公共卫生学院, 山东泰安 271016

收稿日期:2020-03-03 出版日期:2020-04-20 发布日期:2020-04-20
通讯作者: 孟凤霞,Email:mengfengxia@icdc.cn
作者简介:朱彩英,女,在读硕士,主要从事蚊虫抗性分子监测研究,Email:zhucaiying_ivy@163.com
基金资助:
国家科技重大专项（2017ZX10303404002005，2018ZX10101002-002）

Distribution of the ace gene of Aedes albopictus in different geographical populations in China

ZHU Cai-ying¹, ZHAO Chun-chun¹, LIU Qi-yong¹, LI Gui-chang¹, YAN Dong-ming^1,2, SONG Xiu-ping¹, WANG Jun¹, MENG Feng-xia¹

1 Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
2 School of Public Health, Shandong First Medical University

Received:2020-03-03 Online:2020-04-20 Published:2020-04-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404002005, 2018ZX10101002-002)

摘要/Abstract

摘要： 目的了解登革热媒介白纹伊蚊野外种群的乙酰胆碱酯酶（ace）基因在不同区域的基因型及分布特点，从而进行白纹伊蚊对有机磷和氨基甲酸酯类杀虫剂抗药性机制的初步探索。方法 2018年6-9月在海南省海口市、广东省广州市、云南省景洪市和贵州省兴义市的14个采样点（地理种群），共采集白纹伊蚊幼虫、蛹和成蚊422只，经形态学或分子生物学方法鉴定后，用无水乙醇浸泡，并于-20℃冻存备用。提取单只蚊虫的DNA，PCR扩增ace基因部分片段，测序后分析ace基因G119位点突变情况。结果针对上述4个省的14个种群白纹伊蚊ace基因测定，发现白纹伊蚊ace基因G119位点仅存在个别碱基突变，并未发现氨基酸突变。共检测到2种等位基因，即野生型GGA/G（832/98.58%）和突变型GGG/G（12/1.42%）；1种基因型，即野生型纯合子G/G（422/100%）。结论仅有少部分白纹伊蚊个体ace基因G119位点发生了碱基突变，但该碱基替换并未引起相应的氨基酸突变。因此，白纹伊蚊对有机磷和氨基甲酸酯类杀虫剂的靶标抗药性机制亟需进一步探索。

关键词: 白纹伊蚊, 抗药性, 乙酰胆碱脂酶基因, 基因突变

Abstract: Objective To investigate the genotypes and distribution characteristics of the ace gene of the dengue vector Aedes albopictus (field population) in different regions of China, and to preliminarily explore their resistance mechanism to organophosphorus and carbamate insecticides. Methods A total of 422 larvae, pupae, or adults of Ae. albopictus were collected at 14 sampling sites (14 geographical populations) in Haikou of Hainan province, Guangzhou of Guangdong province, Jinghong of Yunnan province, and Xingyi of Guizhou province from June to September 2018, and they were identified by morphology or molecular biology, soaked in anhydrous ethanol, and stored at -20℃. DNA was extracted from individual mosquito, and the partial fragment of the ace gene was amplified by PCR, sequenced, and analyzed for mutation at G119. Results There were only a few base mutations but no amino acid mutations at G119 of the ace gene. Two alleles were detected, namely wild-type GGA/G (832/98.58%) and mutant-type GGG/G (12/1.42%), and one genotype was detected, namely wild-type homozygote G/G (422/100%). Conclusion Only a few individuals of Ae. albopictus showed a base mutation at G119 of the ace gene, but the base substitution did not cause the corresponding amino acid mutation. Therefore, the resistance mechanism of Ae. albopictus to organophosphorus and carbamate insecticides needs to be further explored.

Key words: Aedes albopictus, Insecticide resistance, Ace gene, Gene mutation

中图分类号:

朱彩英, 赵春春, 刘起勇, 李贵昌, 闫冬明, 宋秀平, 王君, 孟凤霞. 我国不同地理种群白纹伊蚊乙酰胆碱酯酶基因分布调查[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 133-136.

ZHU Cai-ying, ZHAO Chun-chun, LIU Qi-yong, LI Gui-chang, YAN Dong-ming, SONG Xiu-ping, WANG Jun, MENG Feng-xia. Distribution of the ace gene of Aedes albopictus in different geographical populations in China[J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 133-136.

参考文献

[1] 吴凡. 中国白纹伊蚊的分布和影响因素及登革热的风险评估研究[D]. 北京:中国疾病预防控制中心,2009. Wu F. A study on the potential distribution of Aedes albopictus and risk forecasting for future epidemics of dengue in China[D]. Beijing:Chinese Center for Disease Control and Prevention,2009.
[2] 李国太,付鸿,郭玉红,等. 甘肃省长江流域地区白纹伊蚊地理分布调查[J]. 中国媒介生物学及控制杂志,2010,21(3):248-249. Li GT,Fu H,Guo YH,et al. Geographical distribution of Aedes albopictus in the Yangtze River basin in Gansu province[J]. Chin J Vector Biol Control,2010,21(3):248-249.
[3] 孙养信,佘建军,孙亮,等. 陕西省白纹伊蚊分布边界及影响因素调查[J]. 中国媒介生物学及控制杂志,2009,20(5):430-432. Sun YX,She JJ,Sun L,et al. Study on dividing line and influence factor of Aedes albopictus in Shaanxi province[J]. Chin J Vector Biol Control,2009,20(5):430-432.
[4] 施耀勇,张军民. 西北地区白纹伊蚊地理分布初步调查[J]. 中华卫生杀虫药械,2012,18(5):420-421. DOI:10.19821/j.1671-2781.2012.05.018. Shi YY,Zhang JM. Preliminary investigation on geographic distribution of Aedes albopictus in Northwest China[J]. Chin J Hyg Insect Equip,2012,18(5):420-421. DOI:10.19821/j.1671-2781.2012.05.018.
[5] 刘转转. 白纹伊蚊感染寨卡病毒和登革病毒的媒介能力研究[D]. 广州:南方医科大学,2017. Liu ZZ. Vector competence of Aedes albopictus infected with Zika and dengue virus[D]. Guangzhou:Southern Medical University,2017.
[6] 张海林,米竹青,张云智. 白纹伊蚊和埃及伊蚊对基孔肯雅病毒的易感性和传播性的研究[J]. 中国病毒学,1994,9(3):195-200. Zhang HL,Mi ZQ,Zhang YZ. Susceptibility and transmissibility of Aedes albopictus and Aedes aegypti mosquitoes to Chikungunya virus[J]. Virol Sin,1994,9(3):195-200.
[7] 黄慕嫦,盛钊君,区博文,等. 江门市白纹伊蚊幼虫的抗药性现状调查[J]. 中华卫生杀虫药械,2018,24(4):345-347. Huang MC,Sheng ZJ,Ou BW,et al. Investigation on insecticide resistance of Aedes albopictus in Jiangmen city[J]. Chin J Hyg Insect Equip,2018,24(4):345-347.
[8] Xia XF,Sun BT,Gurr GM,et al. Gut microbiota mediate insecticide resistance in the diamondback moth,Plutella xylostella (L.)[J]. Front Microbiol,2018,9:25. DOI:10.3389/fmicb.2018.00025.
[9] Dang K,Doggett SL,Singham GV,et al. Insecticide resistance and resistance mechanisms in bed bugs,Cimex spp. (Hemiptera:Cimicidae)[J]. Parasit Vector,2017,10(1):318. DOI:10.1186/s13071-017-2232-3.
[10] 刘宏美,代玉华,公茂庆. 蚊虫抗药性分子机制研究进展[J]. 中国媒介生物学及控制杂志,2012,23(4):366-369. DOI:10.11853/j.issn.1003.8280.2016.05.028. Liu HM,Dai YH,Gong MQ. Progress in molecular mechanisms of mosquito resistance to insecticides[J]. Chin J Vector Biol Control,2012,23(4):366-369. DOI:10.11853/j.issn.1003.8280. 2016.05.028.
[11] Weill M,Lutfalla G,Mogensen K,et al. Insecticide resistance in mosquito vectors[J]. Nature,2003,423(6936):136-137. DOI:10.1038/423136b.
[12] Holt RA,Subramanian GM,Halpern A,et al. The genome sequence of the malaria mosquito Anopheles gambiae[J]. Science,2002,298(5591):129-149. DOI:10.1126/science.1076181.
[13] 张玲敏,吴明玮. 白纹伊蚊乙酰胆碱酯酶基因片段克隆及序列分析[J]. 中国寄生虫病防治杂志,2003,16(1):47-51. DOI:10.3969/j.issn.1673-5234.2003.01. 016. Zhang LM,Wu MW. Clone and sequence analysis of the acetylcholinesterase gene fragment from Aedes albopictus[J]. Chin J Para Dis Control,2003,16(1):47-51. DOI:10.3969/j.issn.1673-5234.2003.01.016.
[14] 邱星辉. 白纹伊蚊抗药性分子机制研究进展[J]. 寄生虫与医学昆虫学报,2019,26(3):194-198. Qiu XH. Current knowledge about the molecular mechanisms underlying insecticide resistance in Aedes albopictus[J]. Acta Parasitol Med Entomol Sin,2019,26(3):194-198.
[15] Alout H,Labbé P,Berthomieu A,et al. Multiple duplications of the rare ace-1 mutation F290V in Culex pipiens natural populations[J]. Insect Biochem Mol Biol,2009,39(12):884-891. DOI:10.1016/j.ibmb.2009.10.005.
[16] Cheung J,Mahmood A,Kalathur R,et al. Structure of the G119S mutant acetylcholinesterase of the malaria vector Anopheles gambiae reveals basis of insecticide resistance[J]. Structure,2018,26(1/2):130-136.e2. DOI:10.1016/j.str.2017.11.021.
[17] 张恒端. 南京市白纹伊蚊种群遗传学研究[D]. 北京:中国人民解放军军事医学科学院,2015. Zhang HD. Study on population genetics characteristics of Aedes albopictus in Nanjing city[D]. Beijing:Academy of Military Medical Sciences,2015.
[18] 李春晓,董言德,赵彤言. 淡色库蚊对残杀威抗性与酯酶和乙酰胆碱酯酶的关系研究[J]. 中国媒介生物学及控制杂志,2007,18(1):9-11. DOI:10.3969/j.issn.1003-4692.2007.01.003. Li CX,Dong YD,Zhao TY. Relationship of esterase,acetylcholinesterase and propoxur-resistance to Culex pipiens pallens[J]. Chin J Vector Biol Control,2007,18(1):9-11. DOI:10.3969/j.issn.1003-4692.2007.01.003.
[19] Feng XY,Yang C,Yang YC,et al. Distribution and frequency of G119S mutation in ace-1 gene within Anopheles sinensis populations from Guangxi,China[J]. Malaria J,2015,14(1):470. Doi:10.1186/s12936-015-1000-0.

我国不同地理种群白纹伊蚊乙酰胆碱酯酶基因分布调查

Distribution of the ace gene of Aedes albopictus in different geographical populations in China

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	孙钦同, 韩英男, 刘言, 王学军, 刘峰, 赖世宏, 景晓. 山东省登革热媒介白纹伊蚊生态学调查分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 16-20.
[2]	李天奇, 刘钦梅, 吴瑜燕, 罗明宇, 王金娜, 龚震宇. 浙江省登革热重点地区2020年白纹伊蚊幼蚊种群密度监测分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 21-24.
[3]	魏超, 马海芳, 廖宁, 熊绮梦. 广西壮族自治区2020－2021年登革热媒介伊蚊密度专项调查结果分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 25-29.
[4]	赵春春, 周欣欣, 李文玉, 伦辛畅, 刘小波, 吴海霞, 王君, 刘起勇, 孟凤霞. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 30-37.
[5]	郑宇婷, 杨春梅, 杨明东, 姜进勇. 云南边境地区登革热媒介伊蚊生态学及抗药性监测[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 38-43.
[6]	涂涛田, 肖汉森, 孟凤霞, 刘起勇, 刘小波, 何亚明, 季恒青. 重庆市2019年登革热暴发疫情应急控制后媒介伊蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 44-47.
[7]	吕文祥, 程鹏, 彭荟, 王海洋, 王海防, 郭秀霞, 张崇星, 刘宏美, 公茂庆, 刘丽娟. 山东省东平湖地区2021年淡色库蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 104-107.
[8]	周欣欣, 李芬, 段文波, 马欣然, 宋秀平, 吴少英, 孟凤霞. 白纹伊蚊电压门控钠离子通道基因克隆及其生物信息学分析[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 672-679.
[9]	薛海滨, 孟金霞, 任乐, 景晓. 山东省临沂市2018－2020年白纹伊蚊幼蚊监测与风险评估[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 686-690.
[10]	张育富, 张守刚, 朱丁, 顾灯安, 葛小伍, 袁建明, 崔倩, 褚宏亮. 江苏省多层住宅居民区不同楼层白纹伊蚊分布调查[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 700-704.
[11]	周毅彬, 朱奕奕, 朱伟, 姚隽一, 朱江, 冷培恩, 吴寰宇. 诱蚊诱卵器监测的空间抽样方法研究[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 749-755.
[12]	朱伟, 刘翔宇, 周毅彬. 绿篱喷洒控制白纹伊蚊密度的时空变化研究[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 772-778.
[13]	郭秀霞, 程鹏, 刘丽娟, 王海防, 张崇星, 王怀位, 公茂庆. 白纹伊蚊紫外光敏感视蛋白基因的克隆及序列特征[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 553-559.
[14]	张家林, 黄发源. 安徽省2015－2019年登革热媒介伊蚊监测结果分析[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 582-585.
[15]	胡远峰, 谭梁飞. 湖北省鄂州市2016－2020年蜚蠊密度及抗药性监测结果分析[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 599-603.