内蒙古地区全沟硬蜱细菌群落结构及多样性分析

doi:10.11853/j.issn.1003.8280.2019.06.003

摘要/Abstract

摘要： 目的了解全沟硬蜱细菌的群落结构及种群多样性。方法应用罗氏454高通量测序技术对内蒙古地区全沟硬蜱细菌16S rDNA基因的V3高变异区段进行测定，分析其细菌的群落结构、种群多样性、优势菌群以及常见病原体的分布。结果获得用于分析的序列和操作分类单元（OTUs）数目分别为22 727条和1 570个；所有样本的覆盖度均≥ 0.95，表明本次测序量充分，基本可覆盖所有细菌物种。其中，雄蜱样本的丰富度指数、多样性指数最高，分别为2 431和4.11；其次为雌蜱和卵。全沟硬蜱中优势菌群依次为变形菌门、厚壁菌门、放线菌门，其中以α-变形菌纲相对丰度最高；在属水平上，不同类别样本中相对丰度较高的菌属组成差异较大。在样本中检测到致病菌塔拉塞维奇立克次体、螺原体和宫本疏螺旋体，环境细菌如金黄杆菌属、欧文菌、短波单胞菌等，以及母系遗传共生菌（Montezuma）。结论内蒙古地区全沟硬蜱不同样本间细菌群落和丰度具有较大差异。

关键词: 全沟硬蜱, 细菌群落结构, 多样性, 16S rDNA, 罗氏454高通量测序

Abstract: Objective To investigate the bacterial community composition and population diversity in Ixodes persulcatus. Methods Roche 454 high-throughput sequencing technology was applied to determine the highly variable V3 segment of 16S rDNA gene of I. persulcatus in Inner Mongolia. Meanwhile, analyses were performed on bacterial community composition, population diversity, dominant bacterial population, and distribution of common pathogens. Results A total of 22 727 sequences and 1 570 operational taxonomic units were obtained for analysis. The coverage of all samples was greater than or equal to 0.95, which indicated that the number of sequences for sequencing was sufficient for covering basically all the bacterial species. Among the samples, male ticks had the highest richness index and diversity index (2 431 and 4.11, respectively), followed by female ticks and eggs. The dominant bacteria in I. persulcatus were Proteobacteria, Firmicutes, and Actinobacteria, sequentially, among which Alphaproteobacteria showed the highest relative abundance. On the genus level, the composition of genera with higher relative abundance varied greatly between different types of samples. Bacteria found in the samples were pathogens (i.e., Candidatus Rickettsia tarasevichiae, Spiroplasma, and Borrelia miyamotoi), environmental bacteria (i.e., Chryseobacterium, Erwinia, and Brevundimonas), and maternally inherited symbiont (i.e., Montezuma). Conclusion There are relatively significant differences in bacterial community composition and abundance between different I. persulcatus samples in Inner Mongolia.

Key words: Ixodes persulcatus, Bacterial community composition, Diversity, 16S rDNA, Roche 454 high-throughput sequencing

中图分类号:

R384.4
R37

李思思, 张晓雨, 张艳凯, 刘敬泽. 内蒙古地区全沟硬蜱细菌群落结构及多样性分析[J]. 中国媒介生物学及控制杂志, 2019, 30(6): 607-612.

LI Si-si, ZHANG Xiao-yu, ZHANG Yan-kai, LIU Jing-ze. An analysis of bacterial community composition and diversity in Ixodes persulcatus in Inner Mongolia, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(6): 607-612.

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