细胞色素P450介导的蚊虫抗药性研究进展

doi:10.11853/j.issn.1003.8280.2019.05.027

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (5): 589-592.DOI: 10.11853/j.issn.1003.8280.2019.05.027

细胞色素P450介导的蚊虫抗药性研究进展

王洋^1,2, 宋晓^1,2, 程鹏², 公茂庆²

1 济南大学, 山东省医学科学院医学与生命科学学院, 山东济南 250062;
2 山东省寄生虫病防治研究所, 山东第一医科大学(山东省医学科学院), 山东济宁 272033

收稿日期:2019-04-18 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: 公茂庆,Email:gmq2005@163.com
作者简介:王洋,男,在读硕士,主要从事媒介昆虫抗药性治理与监测工作,Email:wyyang1221@163.com
基金资助:
国家自然科学基金（81672059，81871685）；山东省重点研发计划（2018GSF118040，2019GSF111006）；山东省医学科学院医药卫生科技创新工程

Research advances in mosquito resistance to insecticides mediated by cytochrome P450

WANG Yang^1,2, SONG Xiao^1,2, CHENG Peng², GONG Mao-qing²

1 School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, Shandong Province, China;
2 Shandong Institute of Parasitic Diseases, Shandong First Medical University & Shandong Academy of Medical Sciences

Received:2019-04-18 Online:2019-10-20 Published:2019-10-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81672059, 81871685), Shandong Province Key Research and Development Projects (No. 2018GSF118040, 2019GSF111006) and the Innovation Project of Shandong Academy of Medical Sciences

摘要/Abstract

摘要： 细胞色素P450是一类广泛分布于生物体内的重要酶系，能够代谢多种内源性和外源性物质，在昆虫对杀虫剂代谢解毒方面起着重要作用。P450介导的杀虫剂代谢解毒作用具有普遍性和交互抗性的特点，其机制涉及酶表达量增加和酶活性改变。该文以蚊虫为例综述了P450介导的抗药性一些新进展。

关键词: 蚊虫, 细胞色素P450, 抗药性, 分子机制

Abstract: Cytochrome P450 is an important family of enzymes ubiquitously expressed in organisms. It can metabolize a variety of endogenous and exogenous substances, and plays an important role in the metabolism and detoxification of insecticides in insects. The metabolism and detoxification of insecticides mediated by P450 are characterized by universality and cross resistance, and the mechanisms involve an increase in enzyme expression and a change in enzyme activity. This article reviews some new research advances in the P450-mediated insecticide resistance of mosquitoes.

Key words: Mosquitoes, Cytochrome P450, Insecticide resistance, Molecular mechanism

中图分类号:

王洋, 宋晓, 程鹏, 公茂庆. 细胞色素P450介导的蚊虫抗药性研究进展[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 589-592.

WANG Yang, SONG Xiao, CHENG Peng, GONG Mao-qing. Research advances in mosquito resistance to insecticides mediated by cytochrome P450[J]. Chines Journal of Vector Biology and Control, 2019, 30(5): 589-592.

参考文献

[1] Wang YL,Wang X,Liu XB,et al. Epidemiology of imported infectious diseases,China,2005-2016[J]. Emerg Infect Dis,2019,25(1):33-41. DOI:10.3201/eid2501.180178.
[2] Zhu F,Lavine L,O'Neal S,et al. Insecticide resistance and management strategies in urban ecosystems[J]. Insects,2016,7(1):2. DOI:10.3390/insects7010002.
[3] 邱星辉. 细胞色素P450介导的昆虫抗药性的分子机制[J]. 昆虫学报,2014,57(4):477-482.
[4] Chiu TL,Wen ZM,Rupasinghe SG,et al. Comparative molecular modeling of Anopheles gambiae CYP6Z1,a mosquito P450 capable of metabolizing DDT[J]. Proc Natl Acad Sci USA,2008,105(26):8855-8860. DOI:10.1073/pnas.0709249105.
[5] Ishak IH,Riveron JM,Ibrahim SS,et al. The Cytochrome P450 gene CYP6P12 confers pyrethroid resistance in kdr-free Malaysian populations of the dengue vector Aedes albopictus[J]. Sci Rep,2016,6:24707. DOI:10.1038/srep24707.
[6] 公茂庆,刘波,胡小邦,等. 淡色库蚊CYP6F1基因的克隆及表达差异的鉴定[J]. 中华卫生杀虫药械,2008,14(2):100-104.
[7] Yahouédo GA,Chandre F,Rossignol M,et al. Contributions of cuticle permeability and enzyme detoxification to pyrethroid resistance in the major malaria vector Anopheles gambiae[J]. Sci Rep,2017,7:11091. DOI:10.1038/s41598-017-11357-z.
[8] Ishak IH,Kamgang B,Ibrahim SS,et al. Pyrethroid resistance in Malaysian populations of dengue vector Aedes aegypti is mediated by CYP9 family of cytochrome P450 genes[J]. PLoS Negl Trop Dis,2017,11(1):e0005302. DOI:10.1371/journal.pntd.0005302.
[9] Stevenson BJ,Pignatelli P,Nikou D,et al. Pinpointing P450s associated with pyrethroid metabolism in the dengue vector,Aedes aegypti:developing new tools to combat insecticide resistance[J]. PLoS Negl Trop Dis,2012,6(3):e1595. DOI:10.1371/journal.pntd.0001595.
[10] Goindin D,Delannay C,Gelasse A,et al. Levels of insecticide resistance to deltamethrin,malathion,and temephos,and associated mechanisms in Aedes aegypti mosquitoes from the Guadeloupe and Saint Martin islands (French West Indies)[J]. Infect Dis Poverty,2017,6:38. DOI:10.1186/s40249-017-0254-x.
[11] Riaz MA,Chandor-Proust A,Dauphin-villemant C,et al. Molecular mechanisms associated with increased tolerance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti[J]. Aquat Toxicol,2013,126:326-337. DOI:10.1016/j.aquatox.2012.09.010.
[12] Nardini L,Hunt RH,Dahan-Moss YL,et al. Malaria vectors in the democratic republic of the Congo:the mechanisms that confer insecticide resistance in Anopheles gambiae and Anopheles funestus[J]. Malar J,2017,16:448. DOI:10.1186/s12936-017-2099-y.
[13] Edi CV,Djogbénou L,Jenkins AM,et al. CYP6 P450 enzymes and ACE-1 duplication produce extreme and multiple insecticide resistance in the malaria mosquito Anopheles gambiae[J]. PLoS Genet,2014,10(3):e1004236. DOI:10.1371/journal.pgen. 1004236.
[14] Ibrahim SS,Amvongo-Adjia N,Wondji MJ,et al. Pyrethroid resistance in the major malaria vector Anopheles funestus is exacerbated by overexpression and overactivity of the P450 CYP6AA1 across Africa[J]. Genes,2018,9(3):140. DOI:10.3390/genes9030140.
[15] Ibrahim SS,Ndula M,Riveron JM,et al. The P450 CYP6Z1 confers carbamate/pyrethroid cross-resistance in a major African malaria vector beside a novel carbamate-insensitive N485I acetylcholinesterase-1 mutation[J]. Mol Ecol,2016,25(14):3436-3452. DOI:10.1111/mec.13673.
[16] Li M,Reid WR,Zhang L,et al. A whole transcriptomal linkage analysis of gene co-regulation in insecticide resistant house flies,Musca domestica[J]. BMC Genomics,2013,14:803. DOI:10.1186/1471-2164-14-803.
[17] 周国理,黄炯烈,吴瑜,等. 白纹伊蚊CYP6N3基因启动子序列的分子克隆与功能分析[J]. 寄生虫与医学昆虫学报,2002,9(1):12-20. DOI:10.3969/j.issn.1005-0507.2002.01.003.
[18] Weedall GD,Mugenzi LMJ,Menze BD,et al. A cytochrome P450 allele confers pyrethroid resistance on a major African malaria vector,reducing insecticide-treated bednet efficacy[J]. Sci Transl Med,2019,11(484):eaat7386. DOI:10.1126/scitranslmed.aat 7386.
[19] Ingham VA,Pignatelli P,Moore JD,et al. The transcription factor Maf-S regulates metabolic resistance to insecticides in the malaria vector Anopheles gambiae[J]. BMC Genomics,2017,18:669. DOI:10.1186/s12864-017-4086-7.
[20] Smith LB,Tyagi R,Kasai S,et al. CYP-mediated permethrin resistance in Aedes aegypti and evidence for trans-regulation[J]. PLoS Negl Trop Dis,2018,12(11):e0006933. DOI:10.1371/journal.pntd.0006933.
[21] Kasai S,Komagata O,Itokawa K,et al. Mechanisms of pyrethroid resistance in the dengue mosquito vector,Aedes aegypti:target site insensitivity,penetration,and metabolism[J]. PLoS Negl Trop Dis,2014,8(6):e2948. DOI:10.1371/journal.pntd. 0002948.
[22] Bariami V,Jones CM,Poupardin R,et al. Gene amplification,ABC transporters and cytochrome P450s:unraveling the molecular basis of pyrethroid resistance in the dengue vector,Aedes aegypti[J]. PLoS Negl Trop Dis,2012,6(6):e1692. DOI:10.1371/journal.pntd.0001692.
[23] Riveron JM,Irving H,Ndula M,et al. Directionally selected cytochrome P450 alleles are driving the spread of pyrethroid resistance in the major malaria vector Anopheles funestus[J]. Proc Natl Acad Sci USA,2013,110(1):252-257. DOI:10.1073/pnas.1216705110.
[24] Ibrahim SS,Riveron JM,Bibby J,et al. Allelic variation of cytochrome P450s drives resistance to bednet insecticides in a major malaria vector[J]. PLoS Genet,2015,11(10):e1005618. DOI:10.1371/journal.pgen.1005618.
[25] Irving H,Riveron JM,Ibrahim SS,et al. Positional cloning of rp2 QTL associates the P450 genes CYP6Z1,CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus[J]. Heredity (Edinb),2012,109(6):383-392. DOI:10.1038/hdy.2012.53.
[26] Audsley N,Down RE. G protein coupled receptors as targets for next generation pesticides[J]. Insect Biochem Mol Biol,2015,67:27-37. DOI:10.1016/j.ibmb.2015.07.014.
[27] Li T,Liu LN,Zhang L,et al. Role of G-protein-coupled receptor-related genes in insecticide resistance of the mosquito,Culex quinquefasciatus[J]. Sci Rep,2014,4:6474. DOI:10. 1038/srep06474.
[28] 孙艳. 视蛋白基因参与蚊抗药性机制的研究[D]. 南京:南京医科大学,2012.
[29] Zhou D,Duan BY,Xu Y,et al. NYD-OP7/PLC regulatory signaling pathway regulates deltamethrin resistance in Culex pipiens pallens (Diptera:Culicidae)[J]. Parasit Vectors,2018,11:419. DOI:10.1186/s13071-018-3011-5.
[30] Liu NN,Li M,Gong YH,et al. Cytochrome P450s-their expression,regulation,and role in insecticide resistance[J]. Pestic Biochem Physiol,2015,120:77-81. DOI:10.1016/j.pestbp. 2015.01.006.
[31] Suwanchaichinda C,Brattsten LB. Genomic and bioinformatic analysis of NADPH-cytochrome P450 reductase in Anopheles stephensi (Diptera:Culicidae)[J]. J Insect Sci,2014,14(1):165. DOI:10.1093/jisesa/ieu027.
[32] Ismail HM,O'Neill PM,Hong DW,et al. Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions[J]. Proc Natl Acad Sci USA,2013,110(49):19766-19771. DOI:10.1073/pnas.1320185110.
[33] Holt RA,Subramanian GM,Halpern A,et al. The genome sequence of the malaria mosquito Anopheles gambiae[J]. Science,2002,298(5591):129-149. DOI:10.1126/science.1076181.
[34] Nene V,Wortman JR,Lawson D,et al. Genome sequence of Aedes aegypti,a major arbovirus vector[J]. Science,2007,316(5832):1718-1723. DOI:10.1126/science.1138878.
[35] Arensburger P,Megy K,Waterhouse RM,et al. Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics[J]. Science,2010,330(6000):86-88. DOI:10.1126/science.1191864.
[36] Chen XG,Jiang XT,Gu JB,et al. Genome sequence of the Asian Tiger mosquito,Aedes albopictus,reveals insights into its biology,genetics,and evolution[J]. Proc Natl Acad Sci USA,2015,112(44):E5907-5915. DOI:10.1073/pnas.1516410112.
[37] Yin CL,Shen GY,Guo DH,et al. InsectBase:a resource for insect genomes and transcriptomes[J]. Nucleic Acids Res,2016,44(D1):D801-807. DOI:10.1093/nar/gkv1204.

细胞色素P450介导的蚊虫抗药性研究进展

Research advances in mosquito resistance to insecticides mediated by cytochrome P450

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