中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (5): 582-585.DOI: 10.11853/j.issn.1003.8280.2019.05.025

• 调查研究 • 上一篇    下一篇

云南省登革热重点地区埃及伊蚊对11种杀虫剂的抗药性调查

兰学梅1, 朱进2, 李华昌3, 高阳2, 邹建红4, 谭力滔4, 普金文5, 杨明东1   

  1. 1 云南省寄生虫病防治所, 云南省虫媒病毒研究中心, 云南省虫媒传染病防控研究重点实验室, 金宁一院士工作站, 面向南亚东南亚热带病国际科技人员交流与教育培训基地, 云南 普洱 665099;
    2 西双版纳傣族自治州疾病预防控制中心, 云南 景洪 666100;
    3 临沧市疾病预防控制中心, 云南 临沧 677000;
    4 景洪市疾病预防控制中心, 云南 景洪 666100;
    5 耿马傣族佤族自治县疾病预防控制中心, 云南 耿马 677500
  • 收稿日期:2019-06-14 出版日期:2019-10-20 发布日期:2019-10-20
  • 通讯作者: 杨明东,Email:quanjueyang@163.com
  • 作者简介:兰学梅,女,医师,从事媒介生物学与控制研究,Email:lanxuemei0715@163.com
  • 基金资助:
    云南省科技计划项目青年项目(2017FD189);云南省生物医药重大专项(2017ZF007);国家重点研发计划(2016YFC1200500)

An investigation of the resistance of Aedes aegypti to 11 insecticides in key areas of dengue fever in Yunnan province, China

LAN Xue-mei1, ZHU Jin2, LI Hua-chang3, GAO Yang2, ZOU Jian-hong4, TAN Li-tao4, PU Jin-wen5, YANG Ming-dong1   

  1. 1 Yunnan Institute of Parasitic Diseases, Yunnan Provincial Center of Arborvirus Research, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Academician Workstation of Professor Jin Ningyi, Training Base of International Scientific Exchange and Education in Tropical Diseases for South and Southeast Asia, Pu'er 665099, Yunnan Province, China;
    2 Xishuangbanna Dai Autonomous Prefecture Center for Disease Control and Prevention;
    3 Lincang Center for Disease Control and Prevention;
    4 Jinghong Center for Disease Control and Prevention;
    5 Gengma Dai and Wa Autonomous County Center for Disease Control and Prevention
  • Received:2019-06-14 Online:2019-10-20 Published:2019-10-20
  • Supported by:
    Supported by the Yunnan Science and Technology Plan Project Youth Project (No. 2017FD189), Yunnan Province Biomedical Major Project (No. 2017ZF007) and National Key Research and Development Plan (No. 2016YFC1200500)

摘要: 目的 了解云南省登革热重点地区埃及伊蚊对11种杀虫剂的抗药性水平。方法 2017年8-9月分别从景洪市城区和耿马傣族佤族自治县(耿马县)孟定镇采集埃及伊蚊蛹或幼虫,在实验室繁殖至子一代(F1),采用成蚊滤纸接触筒法,测定其对11种杀虫剂的抗药性。结果 景洪市城区埃及伊蚊对氯菊酯、氯氟氰菊酯、高效氯氰菊酯、溴氰菊酯、顺式氯氰菊酯、马拉硫磷、杀螟硫磷、恶虫威、残杀威、氟虫腈和滴滴涕(DDT)的24 h后校正死亡率分别为0.92%、0、3.19%、91.40%、20.43%、85.86%、100%、97.71%、96.67%、99.41%和1.96%,KT50分别为>60、>60、53.83、45.39、48.25、>60、>60、36.34、32.13、>60 min和KT50无法计算;耿马县孟定镇埃及伊蚊对上述常用杀虫剂的24 h后校正死亡率分别为1.43%、3.01%、11.35%、60.27%、5.96%、100%、100%、100%、95.68%、95.95%和0.74%,KT50分别为>60、>60、26.48、16.35、22.67、>60、>60、35.03、40.17、>60 min和KT50无法计算。结论 景洪市和孟定镇埃及伊蚊对拟除虫菊酯类和有机氯类(DDT)杀虫剂均产生了不同程度的抗药性,对有机磷类、氨基甲酸酯类和苯基吡唑类(氟虫腈)杀虫剂仍然敏感。应因地制宜,停用或者限制使用已经产生抗性的杀虫剂。

关键词: 埃及伊蚊, 生物学测定, 抗药性

Abstract: Objective To investigate the resistance level of Aedes aegypti to 11 insecticides in the key areas of dengue fever in Yunnan province, China. Methods From August 2017 to September 2017, the pupae or larvae of Ae. aegypti were collected in Jinghong city and Mengding town, Gengma Dai and Wa Autonomous County, and then they were bred to the first generation (F1) in the laboratory. The resistance of the first generation to 11 insecticides was determined by the filter paper contact method. Results The 24 h corrected mortality rates of adult Ae. aegypti in Jinghong city to permethrin, λ-cyhalothrin, beta-cypermethrin, deltamethrin, α-cypermethrin, malathion, fenitrothion, bendiocarb, propoxur, fipronil, and DDT were 0.92%, 0, 3.19%, 91.40%, 20.43%, 85.86%, 100%, 97.71%, 96.67%, 99.41%, and 1.96%, respectively, and KT50 was >60, >60, 53.83, 45.39, 48.25, >60, >60, 36.34, 32.13, >60 min, and not calculable, respectively. The 24 h mortality rates of adult Ae. aegypti in Mengding town to the above mentioned insecticides were 1.43%, 3.01%, 11.35%, 60.27%, 5.96%, 100%, 100%, 100%, 95.68%, 95.95%, and 0.74%, respectively, and the corresponding KT50 was >60, >60, 26.48, 16.35, 22.67, >60, >60, 35.03, 40.17, >60 min, and not calculable, respectively. Conclusion Aedes aegypti in Jinghong city and Mengding town have developed different degrees of resistance to pyrethroids and DDT insecticides, but still present high sensitivity to malathion, fenitrothion, propoxur, bendiocarb and fipronil. Measures should be carried out accordingly to limit or stop the use of insecticides to which Ae. aegypti has already developed resistance.

Key words: Aedes aegypti, Bioassay, Insecticide resistance

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