北京市温带臭虫击倒抗性基因突变检测

doi:10.11853/j.issn.1003.8280.2019.05.010

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (5): 524-527.DOI: 10.11853/j.issn.1003.8280.2019.05.010

北京市温带臭虫击倒抗性基因突变检测

刘念¹, 周小洁², 戚广浩³, 李梅¹, 邱星辉¹

1 中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101;
2 北京市疾病预防控制中心, 北京 100013;
3 北京市通州区疾病预防控制中心, 北京 101100

收稿日期:2019-04-25 出版日期:2019-10-20 发布日期:2019-10-20
通讯作者: 邱星辉,Email:qiuxh@ioz.ac.cn
作者简介:刘念,女,在读硕士,主要从事昆虫抗药性研究,Email:nianliu95@163.com

Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China

LIU Nian¹, ZHOU Xiao-jie², QI Guang-hao³, LI Mei¹, QIU Xing-hui¹

1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
2 Beijing Center for Disease Control and Prevention;
3 Tongzhou District Center for Disease Control and Prevention

Received:2019-04-25 Online:2019-10-20 Published:2019-10-20

摘要/Abstract

摘要： 目的对温带臭虫电压门控钠离子通道（vgsc）基因进行测序分析，检测抗药性相关的基因突变，为该地区的臭虫治理提供依据。方法利用DNA测序技术，获得vgsc基因片段序列，分析击倒抗性相关位点的基因型。结果与参照品系相应的序列比对，现场采集的6只温带臭虫的vgsc基因序列均存在有义突变，导致编码的电压门控钠离子通道（VGSC）的氨基酸替换，即V419L和L925I突变，突变频率为100%。结论调查的样品均携带可导致击倒抗性的VGSC在419和925两个位点的突变，表明该温带臭虫种群对拟除虫菊酯类杀虫剂具有较高水平抗性。

关键词: 温带臭虫, 电压门控钠离子通道, 基因突变, 击倒抗性, 拟除虫菊酯类杀虫剂

Abstract: Objective To analyze genetic mutations, predict the level of resistance to pyrethroid insecticides in the bedbug Cimex lectularius and provide a guide for bed bug control in this region. Methods Partial sequences of vgsc gene that encodes the target of pyrethroid insecticides (voltage gated sodium channel) were obtained by DNA sequencing, and the knockdown resistance-related mutations were examined. Results The six bed bugs collected on site were found to have genetic mutations at codons 419 and 925, leading to double amino acid substitutions (V419L and L925I) with a frequency of 100%. Conclusion All the investigated bed bugs carry kdr mutations at positions 419 and 925 of the voltage gated sodium channel, predicting that this bed bug population has a higher level of resistance to pyrethroid insecticides.

Key words: Cimex lectularius, Voltage-gated sodium channel, Genetic mutation, Knockdown resistance, Pyrethroid insecticides

中图分类号:

刘念, 周小洁, 戚广浩, 李梅, 邱星辉. 北京市温带臭虫击倒抗性基因突变检测[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 524-527.

LIU Nian, ZHOU Xiao-jie, QI Guang-hao, LI Mei, QIU Xing-hui. Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(5): 524-527.

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北京市温带臭虫击倒抗性基因突变检测

Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China

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