致倦库蚊抗性种群和敏感品系中转铁蛋白基因表达量的分析

doi:10.11853/j.issn.1003.8280.2019.01.011

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (1): 50-55.DOI: 10.11853/j.issn.1003.8280.2019.01.011

致倦库蚊抗性种群和敏感品系中转铁蛋白基因表达量的分析

杨会娜^1,2, 陆靖¹, 谭文彬³

1 济南大学, 山东省医学科学院医学与生命科学学院, 山东济南 250001;
2 济宁市第一人民医院, 山东济宁 272011;
3 济宁医学院基础医学院, 山东济宁 272067

收稿日期:2018-09-03 出版日期:2019-02-20 发布日期:2019-02-20
通讯作者: 谭文彬,Email:63256612@163.com
作者简介:杨会娜,女,在读硕士,主要从事病原生物学研究,Email:394818635@qq.com
基金资助:
国家自然科学基金（81271876，81471985，81672059）；山东省自然科学基金（ZR2015YL023）；山东省高等学校科技发展计划（J13LK12）；山东省高等学校青年骨干教师国内访问学者项目；山东省高校优秀科研创新团队计划

An analysis of transferrin gene expression in resistant population and susceptible population of Culex pipiens quinquefasciatus

YANG Hui-na^1,2, LU Jing¹, TAN Wen-bin³

1 School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250001, Shandong Province, China;
2 Jining First People's Hospital;
3 Academy of Basic Medicine, Jining Medical University

Received:2018-09-03 Online:2019-02-20 Published:2019-02-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81271876, 81471985, 81672059), the Natural Science Foundation of Shandong Province of China (No. ZR2015YL023), Shandong Province Higher Educational Science and Technology Program (No. J13LK12), Program of Domestic Study for Young Scholar of University in Shandong Province Sponsored by Shandong Provincial Educational Department and the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province

摘要/Abstract

摘要： 目的分析转铁蛋白基因在致倦库蚊敏感品系、抗性种群体内的表达量。方法设计引物、提取RNA、扩增目的基因部分基因片段、纯化测序、序列比对、荧光定量PCR。采用t检验对敏感品系和抗性种群致倦库蚊体内转铁蛋白的基因表达量进行统计分析。结果扩增致倦库蚊转铁蛋白部分基因片段，测序后序列比对，荧光定量PCR分析显示，致倦库蚊敏感品系转铁蛋白荧光定量（T_f敏）和抗性种群转铁蛋白荧光定量（T_f抗）平均值分别为15.21和18.41，内参基因荧光定量（β_敏和β_抗）平均值分别为20.53和19.10，转铁蛋白基因在致倦库蚊抗性种群中的表达量降低，2个样本体内转铁蛋白基因表达差异有统计学意义（t=10.390，P<0.001）。结论致倦库蚊转铁蛋白基因可作为蚊虫抗性检测及治理的靶标基因。

关键词: 致倦库蚊, 转铁蛋白, 荧光定量聚合酶链式反应

Abstract: Objective To analyze the expression level of transferrin gene in the susceptible population and resistant population of Culex pipiens quinquefasciatus. Methods RNA was extracted and was amplified by quantitative real-time PCR (qRT-PCR) to produce partial gene fragments of the target gene using the designed primers. After purification and sequencing, the sequence alignment was performed. The t test was used to statistically analyze the expression levels of transferrin gene in the susceptible population and resistant population of Cx. pipiens quinquefasciatus. Results The qRT-PCR analysis showed that the mean fluorescence quantification values of transferring gene in susceptible population and resistant population of Cx. pipiens quinquefasciatus were 15.21 and 18.41, respectively; the mean fluorescence quantification values of the internal reference gene in the two populations were 20.53 and 19.10, respectively. The expression level of transferrin gene in the resistant population of Cx. pipiens quinquefasciatus was reduced, and there was a significant difference in the expression level of transferrin gene between the two populations (t=10.390, P<0.001). Conclusion The transferrin gene of Cx. pipiens quinquefasciatus can be used as a target gene for the detection and treatment of mosquito resistance.

Key words: Culex pipiens quinquefasciatus, Transferrin, Quantitative real-time polymerase chain reaction

中图分类号:

杨会娜, 陆靖, 谭文彬. 致倦库蚊抗性种群和敏感品系中转铁蛋白基因表达量的分析[J]. 中国媒介生物学及控制杂志, 2019, 30(1): 50-55.

YANG Hui-na, LU Jing, TAN Wen-bin. An analysis of transferrin gene expression in resistant population and susceptible population of Culex pipiens quinquefasciatus[J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 50-55.

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致倦库蚊抗性种群和敏感品系中转铁蛋白基因表达量的分析

An analysis of transferrin gene expression in resistant population and susceptible population of Culex pipiens quinquefasciatus

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