关键词: 种群遗传, 同源性, 遗传距离, 系统发生分析

Abstract: Objective To investigate the difference in the sequence of cytochrome C oxidase subunit I (COI) gene in Ixodes persulcatus from the Heilongjiang Suifenhe and Jilin Yanbian ports in China and foreign countries, and to provide a basis for the prevention and control of tick-borne diseases. Methods From June 2013 to August 2016, a total of 106 I. persulcatus ticks were captured at Heilongjiang Suifenhe and Jilin Yanbian ports, among which 31 were selected to extract genomic DNA. PCR was used for the amplification of the COI gene, and then a homology analysis was performed, a phylogenetic tree was established, and a genetic distance analysis was performed for two geographical populations of I. persulcatus. Results The sequence of the COI gene in I. persulcatus from Heilongjiang Suifenhe and Jilin Yanbian ports had a homology of 99%-100% with the sequence of the COI gene in I. persulcatus from Russia and the Altai region in the border of Kazakhstan. The genetic distance of I. persulcatus from Suifenhe and Yanbian ports was ≤ 0.003. Conclusion The sequence of the COI gene in I. persulcatus from Suifenhe and Yanbian ports in China is highly consistent with that from neighboring countries. There may exist a high level of gene exchange or migration.

Key words: Population genetics, Homology, Genetic distance, Phylogenetic analysis