云南省蛛蝇和蝠蝇感染沃尔巴克氏体初步调查研究

doi:10.11853/j.issn.1003.8280.2019.01.004

摘要/Abstract

摘要： 目的了解云南省蛛蝇和蝠蝇中沃尔巴克氏体（Wolbachia）的感染状况及其基因型别。方法 2012－2017年，在云南省芒市、腾冲、畹町、孟连、勐腊、祥云、墨江、永德县和保山市隆阳区共9个采样点采集蝙蝠体表蛛蝇和蝠蝇，PCR扩增沃尔巴克氏体的核糖体小亚基16S（16S rRNA）和细菌表皮蛋白（wsp）基因并测序，对所有16S rRNA和wsp基因进行系统进化分析。结果 2012－2017年，在云南省9个采集点采集蝙蝠体表蛛蝇747只（48批）和蝠蝇19只（6批），检测54批标本获得沃尔巴克氏体16S rRNA和wsp基因序列各24条。云南省蝙蝠体表蛛蝇和蝠蝇中，蛛虱蝇属（Phthiridium）6批和Eucampsipoda属37批中检出沃尔巴克氏体阳性分别为2批（2/6）和19批（19/37），蛛虱蝇属（Nycteribia）（0/4）和笔虱蝇属（Penicillidia）（0/1）未检出；蝠蝇均属于蝠虱蝇属（Brachytarsina），6批中检出沃尔巴克氏体阳性的有3批（3/6）。16S rRNA基因系统进化结果提示，24株沃尔巴克氏体均位于一个大的分支中，与1株来源于白蚁（DQ837204）的同源性最高达98.80%～100%。wsp基因的系统进化分析提示，24株沃尔巴克氏体位于B组和F组基因型。结论沃尔巴克氏体在云南省蛛蝇和蝠蝇中广泛而长期存在，wsp基因系统进化分析属于B组和F组基因型。

关键词: 沃尔巴克氏体, 蛛蝇, 蝠蝇

Abstract: Objective To investigate the infection status and genotypes of Wolbachia in Nycteribiidae and Streblidae in Yunnan province, China. Methods From 2012 to 2017, Nycteribiidae and Streblidae were collected from bats in 9 spots in Yunnan province, i.e., Mangshi, Tengchong, Wanding, Menglian, Mengla, Xiangyun, Mojiang, Yongde county, and Baoshan city. The 16S rRNA and wsp genes of Wolbachia were amplified and sequenced by PCR. Phylogenetic analyses were performed on all the 16S rRNA and wsp genes. Results From 2012 to 2017, a total of 747 (48 batches) Nycteribiidae flies and 19 (6 batches) Streblidae flies were collected from the fur of bats in the 9 collection spots in Yunnan province. A total of 24 pairs of 16S rRNA and wsp genes of Wolbachia were identified in the 54 batches of samples. For the Nycteribiidae from the fur of bats in Yunnan province, Wolbachia infection was positive in 2 out of 6 batches of Phthiridium and 19 out of 37 batches of Eucampsipoda, but negative in any batch of Nycteribia (0/4) and Penicillidia (0/1). All 6 batches of Streblidae belonged to Brachytarsina, 3 out of which were positive for Wolbachia infection. Phylogenetic analysis of the 16S rRNA gene showed that the 24 Wolbachia strains shared the same evolutionary branch with one from termites (DQ837204), with a 98.80%-100% homology in nucleotide sequence. Phylogenetic analysis of the wsp gene revealed that the 24 Wolbachia strains belonged to the supergroups B and F. Conclusion Wolbachia has been widely distributed in Nycteribiidae and Streblidae in Yunnan province for a long time. Phylogenetic analysis of the wsp sequences suggests that all identified Wolbachia strains belong to the supergroups B and F.

Key words: Wolbachia, Nycteribiidae, Streblidae

中图分类号:

R384.2
R376

韩茜, 冯云, 杨卫红, 张婧, 潘虹, 杨丽芬, 王娟. 云南省蛛蝇和蝠蝇感染沃尔巴克氏体初步调查研究[J]. 中国媒介生物学及控制杂志, 2019, 30(1): 18-24.

HAN Xi, FENG Yun, YANG Wei-hong, ZHANG Jing, PAN Hong, YANG Li-fen, WANG Juan. A preliminary study of Wolbachia infection in Nycteribiidae and Streblidae in Yunnan province, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 18-24.

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