应用TaqMan探针实时荧光定量PCR法调查北京市部分城郊区鼠传病原体流行状况

doi:10.11853/j.issn.1003.8280.2019.01.003

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (1): 12-17.DOI: 10.11853/j.issn.1003.8280.2019.01.003

应用TaqMan探针实时荧光定量PCR法调查北京市部分城郊区鼠传病原体流行状况

栗冬梅¹, 徐兆楠², 朱彩英¹, 张政², 康央^1,3, 张洪江², 宋秀平¹, 唐承军², 葛军旗²

1 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 感染性疾病诊治协同创新中心, 北京 102206;
2 北京市朝阳区疾病预防控制中心, 北京 100021;
3 山东大学生命科学学院, 山东济南 250100

收稿日期:2018-09-04 出版日期:2019-02-20 发布日期:2019-02-20
通讯作者: 葛军旗,Email:18785550@qq.com;栗冬梅,Email:lidongmei@icdc.cn
作者简介:栗冬梅,女,研究员,主要从事巴尔通体及相关传染病防治研究,Email:lidongmei@icdc.cn
基金资助:
北京市自然科学基金（7173262）；国家科技重大专项（2017ZX10303404）；传染病预防控制国家重点实验室面上项目（2018SKLID802）

An epidemiologic investigation of rodent-borne pathogens in some suburban areas of Beijing, China

LI Dong-mei¹, XU Zhao-nan², ZHU Cai-ying¹, ZHANG Zheng², KANG Yang^1,3, ZHANG Hong-jiang², SONG Xiu-ping¹, TANG Cheng-jun², GE Jun-qi²

1 State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Chaoyang District Center for Disease Control and Prevention of Beijing;
3 School of Life Sciences, Shandong University

Received:2018-09-04 Online:2019-02-20 Published:2019-02-20
Supported by:
Supported by the Municipal Natural Science Foundation of Beijing of China (No. 7173262), National Science and Technology Major Project of China (No. 2017ZX10303404) and the Project of State Key Laboratory of Infectious Disease Prevention and Control (No. 2018KLID802)

摘要/Abstract

摘要： 目的调查北京市部分城郊区6种鼠传病原体流行状况。方法将已报道的TaqMan探针实时荧光定量PCR（qPCR）检测方法在本实验室进行评估，制备质粒标准品和绘制标准曲线，依据重新评估的数据确定结果的检测阈值。2017年1－6月应用夹夜法在北京市朝阳、怀柔、门头沟和平谷4个地区捕获啮齿动物，采集组织样品提取核酸，应用上述qPCR方法检测病原体。结果共捕获啮齿动物160只，其中褐家鼠59只、小家鼠69只、朝鲜姬鼠4只、北社鼠25只和鼩鼱3只。检测动物脾组织结果显示，除鼩鼱外其他啮齿动物均分别感染莫氏立克次体（8.1%）、恙虫病东方体（1.3%）、嗜吞噬细胞无形体（5.6%）、钩端螺旋体（18.7%）和巴尔通体（5.0%）；所有动物中均未检测到土拉弗朗西斯菌。褐家鼠和小家鼠中存在钩端螺旋体、嗜吞噬细胞无形体等多种病原体复合感染情况。结论北京地区的啮齿动物可携带多种常见、新发及再发传染病病原体，应关注并加强该地区这些病原体动物宿主和传播媒介的分布调查及相关传染病的防制工作。

关键词: 啮齿动物, 鼠传病原体, 新发传染病, 实时荧光定量PCR

Abstract: Objective To investigate the prevalence of 6 rodent-borne pathogens, in some suburban areas of Beijing, China. Methods TaqMan fluorescent probe-based quantitative real-time PCR (qPCR) was used for laboratory evaluation. Plasmid standards were prepared and standard curves were plotted. Detection thresholds were determined according to the reevaluated data. From January to June, 2017, the trap-at-night method was used to capture rodents in Chaoyang, Huairou, Mentougou, and Pinggu districts of Beijing, tissue samples were collected to extract nucleic acids, and qPCR was used to detect pathogens. Results A total of 160 rodents were captured, among which there were 59 Rattus norvegicus rodents, 69 Mus musculus rodents, 4 Apodemus peninsulae rodents, 25 Niviventer confucianus, and 3 Sorex spp.. The examination of spleen tissues showed that all rodents except Sorex were infected with Rickettsia typhi (8.1%), Orientia tsutsugamushi (1.3%), Anaplasma phagocytophilum (5.6%), Leptospira interrogans (18.7%), or Bartonella spp. (5.0%). No Francisella tularensis was detected in all rodents. There were co-infections with various pathogens including Leptospira interrogans and A. phagocytophilum in R. norvegicus and M. musculus. Conclusion Rodents in Beijing carry the pathogens of common, emerging and reemerging infectious diseases. The distribution investigation of animal hosts and vectors of these pathogens in these areas and prevention and treatment of related infectious diseases should be strengthened.

Key words: Rodent, Rodent-borne pathogen, Emerging infectious disease, Quantitative real-time PCR

中图分类号:

S443
R37

栗冬梅, 徐兆楠, 朱彩英, 张政, 康央, 张洪江, 宋秀平, 唐承军, 葛军旗. 应用TaqMan探针实时荧光定量PCR法调查北京市部分城郊区鼠传病原体流行状况[J]. 中国媒介生物学及控制杂志, 2019, 30(1): 12-17.

LI Dong-mei, XU Zhao-nan, ZHU Cai-ying, ZHANG Zheng, KANG Yang, ZHANG Hong-jiang, SONG Xiu-ping, TANG Cheng-jun, GE Jun-qi. An epidemiologic investigation of rodent-borne pathogens in some suburban areas of Beijing, China[J]. Chines Journal of Vector Biology and Control, 2019, 30(1): 12-17.

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应用TaqMan探针实时荧光定量PCR法调查北京市部分城郊区鼠传病原体流行状况

An epidemiologic investigation of rodent-borne pathogens in some suburban areas of Beijing, China

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