关键词: 热带臭虫, 温带臭虫, 形态与分子鉴定

Abstract: Objective To establish a method for the morphological and molecular identification of Cimex hemipterus and C. lectularius. Methods In July 2017, C. hemipterus were found and collected in a dormitory in a university in Shenzhen and C. lectularius were found and collected in a dormitory in a university in Tai'an, Shandong. The morphological characteristics of C. hemipterus and C. lectularius were compared and described. Neighbor-Joining (NJ) phylogenetic tree were constructed with mitochondrial gene COI, 16S rDNA, and ribosomal genes 28S rDNA, ITS2. Results Although the adults of C. hemipterus and C. lectularius were similar in external morphology, but they still can be identified and separated by the detailed characteristics differences on their prodorsum, elytra, leg and abdomen. The differences of intra-species DNA sequences based on four genes were less than 2%, and differences of inter-species DNA sequence based on four genes were more than 2%. In all the four NJ trees, C. hemipterus and C. lectularius were clustered into one single branch separately, and the support rate were 100%. Conclusion Cimex hemipterus and C. lectularius can be identified by their external morphology, and can be identified with the common adopted molecular marker. Molecular identification is a good tool to test the results of morphology. Combining the morphology and molecular identification together can provide a conclusive evidence for the morphological closely related species.

Key words: Cimex hemipterus, Cimex lectularius, Morphology and molecular identification