关键词: 巴尔通体, 实时荧光定量PCR, 古尔图地区, 感染调查

Abstract: Objective To understand the infection status of Bartonella in Citellus undulatus from Guertu, and to provide the basis for risk early warning and control measures of rodent-borne diseases. Methods In total, 86 C. undulatus were collected from Chagangguole, Bulanbulake and Baishitou by bow-shaped clip method during 24 hours, the kidneys of the captured rodents were sampled under aseptic condition, genome DNA was extracted and detected by real-time fluorescence quantitative PCR targeting ssrA of Bartonella. The rate of infection were calculated and analyzed by cp test (cp value). Results A total of 86 C. undulatus were tested, the infection rate of Bartonella in those rodents was 48.84% (42/86). The cp of positive samples had the highest value of 37.83 and the lowest value of 27.49, 88.10% of positive samples was in the "35.00-37.83" range. The infection rate of Chagangguole, Bulanlulake and Baishitou were 48.48%, 60.87%, 40.00%, respectively. The flea infection rate and flea index in Bulanlulake were higher than those of other places, the infection rate had no correlation with the flea infection in Guertu (P>0.05). Conclusion There were natural infection of Bartonella among C. undulatus in Guertu, with tendency of wide spread, but pathogen loads were relatively low in wild rodents.

Key words: Bartonella, Real-time fluorescence quantitative PCR, Guertu township, Infection survey