中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (5): 458-461.DOI: 10.11853/j.issn.1003.8280.2018.05.009

• 论著 • 上一篇    下一篇

DNA条形码技术在臭虫物种鉴定中的应用

张瑞玲1,2, 黄振东1, 刘同凯1, 赵爱华3, 张忠1,2   

  1. 1 泰山医学院基础医学院病原生物学教研室, 山东 泰安 271016;
    2 泰山医学院新发传染病溯源及防控协同创新中心, 山东 泰安 271016;
    3 泰安市疾病预防控制中心, 山东 泰安 271000
  • 收稿日期:2018-06-12 出版日期:2018-10-20 发布日期:2018-10-20
  • 通讯作者: 张忠,Email:zhangz@tsmc.edu.cn
  • 作者简介:张瑞玲,女,讲师,主要从事媒介生物学的分子系统学研究,Email:zhangrl_06@126.com
  • 基金资助:
    国家自然科学基金(81401693,81572028)

Application of DNA barcoding in species identification of bedbugs

ZHANG Rui-ling1,2, HUANG Zhen-dong1, LIU Tong-kai1, ZHAO Ai-hua3, ZHANG Zhong1,2   

  1. 1 Department of Pathogenic Biology of Taishan Medical University, Tai'an 271016, Shandong Province, China;
    2 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University;
    3 Tai′an Center for Disease Control and Prevention
  • Received:2018-06-12 Online:2018-10-20 Published:2018-10-20
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. 81401693, 81572028)

摘要: 目的 准确鉴定臭虫样品,为制定和实施有效预防措施提供参考依据。方法 联合利用形态学鉴定和基于线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因片段遗传距离的DNA条形码技术对山东省泰安市某学校发现的臭虫样品进行鉴定。结果 臭虫样品的前胸背板宽约为身长的1/2,与温带臭虫的特征相符。遗传距离分析显示,实验样品与温带臭虫的遗传距离为0~1.3%,与热带臭虫的遗传距离为27.6%~28.0%。结论 形态鉴定和DNA条形码分析结果均表明该实验样品为温带臭虫。臭虫通常是群居生活,且易于借助人类活动进行传播,长期、合理的防制是减少或避免臭虫危害的关键。

关键词: 温带臭虫, 细胞色素C氧化酶亚基Ⅰ基因, 遗传距离

Abstract: Objective To make accurate identification of bedbug species and provide information for implementation of effective prevention and control measures. Methods In this study, morphological identification and DNA barcoding that distinguishes species based on the differential of genetic distances of mitochondrial Cytochrome C oxidase subunit Ⅰgene (COⅠ), were employed to identify the bedbugs collected from a school of Tai'an in Shandong province. Results Firstly, length of pronotum is two times of width, corresponding with the description of Cimex lectularius. Secondly, genetic distances of unidentified bedbug samples with C. lectularius were 0-1.3%, while 27.6%-28.0% with C. hemipterus. Conclusion Both morphological characters and genetic distances supported that these bedbug samples belong to C. lectularius. Bedbugs are social insects, which is usually spread by human activities. Implementation of long term effective prevention and control measures are crucial for reducing and avoiding the threat caused by bedbugs.

Key words: Cimex lectularius, Cytochrome C oxidase subunit Ⅰ gene, Genetic distance

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