关键词: 家蝇, 盐酸环丙沙星, 总超氧化物歧化酶, 过氧化氢酶, 过氧化物酶

Abstract: Objective To investigate the effects of ciprofloxacin on the antioxidases activities of Musca domestica larvae and pupae. Methods T-SOD, CAT, and POD activities were detected and analyzed every day. The housefly larvae being fed with 0.1%, 0.3%, and 0.5% ciprofloxacin, named 0.l% ciprofloxacin group, 0.3% ciprofloxacin group and 0.5% ciprofloxacin group, respectively. Fly larvae fed with normal feed named control group. The detection kits of T-SOD, CAT, and POD were used to detect the enzymatic activity of different groups. The assay on enzymes enzymatic activity of different samples was repeated five times. The statistical methods was used for multiple comparison. Results The enzymatic activities of T-SOD, CAT, and POD were had no significant differences among control groups, 0.1% and 0.3% ciprofloxacin groups. But the enzymatic activities of T-SOD, CAT, and POD at 0.5% ciprofloxacin group were significantly higher than the other three groups. After being fed with 0.5% ciprofloxacin, the T-SOD activity of larvae and pupae were up to 30 and 40 times higher than the control groups, respectively. The activity of CAT in 0.5% ciprofloxacin group of larvae and pupae were up to 3 and 6 times higher than the control groups. In 0.5% ciprofloxacin group, the POD activity of larvae and pupae were up to 2 and 7 times higher than the control groups, respectively. Conclusion Low concentrations of ciprofloxacin had no significant impact on the antioxidase activities of housefly larvae and pupae, high concentration of ciprofloxacin did activate the antioxidase activity.

Key words: Musca domestica, Ciprofloxacin, Total superoxide dismutase, Catalase, Peroxidase