丽蝇蛹集金小蜂和球孢白僵菌对家蝇的协同控制作用

doi:10.11853/j.issn.1003.8280.2018.04.003

中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (4): 336-339.DOI: 10.11853/j.issn.1003.8280.2018.04.003

丽蝇蛹集金小蜂和球孢白僵菌对家蝇的协同控制作用

黄振东^1,2, 万晴^1,2, 庄桂芬^1,2, 薛志静^1,2, 张瑞玲^1,2, 张忠^1,2

1 山东省新发传染病溯源与防控协同创新中心, 山东泰安 271016;
2 泰山医学院基础医学院, 山东泰安 271016

收稿日期:2018-02-14 出版日期:2018-08-20 发布日期:2018-08-20
通讯作者: 张忠,Email:nasonia@163.com
作者简介:黄振东,在读硕士,主要从事病媒生物与虫媒病相关研究,Email:731878607@qq.com
基金资助:
国家自然科学基金（81401693，81572028，81271874）；山东省科技发展计划项目（2014GSF121007）

Synergestic control efficacy of Nasonia vitripennis and Beauveria bassiana against Musca domestica

HUANG Zhen-dong^1,2, WAN Qing^1,2, ZHUANG Gui-fen^1,2, XUE Zhi-jing^1,2, ZHANG Rui-ling^1,2, ZHANG Zhong^1,2

1 Shandong Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Tai'an 271016, Shandong Province, China;
2 School of Basic Medical Sciences of Taishan Medical University

Received:2018-02-14 Online:2018-08-20 Published:2018-08-20
Supported by:
Supported by the National Natural Science Foundation of China(No. 81401693, 81572028, 81271874) and the Science and Technology Development Projects of Shandong Province(No. 2014GSF121007)

摘要/Abstract

摘要： 目的探讨丽蝇蛹集金小蜂和球孢白僵菌对家蝇的协同控制作用，为家蝇防制提供科学依据。方法通过"Y"形管气味选择和寄生选择实验，研究表面黏附球孢白僵菌孢子对丽蝇蛹集金小蜂雌蜂寄主选择性和寄生的影响；利用生态模拟实验研究蝇蛹表面喷洒孢子对羽化家蝇生殖能力和寿命的影响。数据分析采用SPSS 20.0软件进行单因素方差分析。结果丽蝇蛹集金小蜂雌蜂对表面喷洒球孢白僵菌孢子后0、1、3、5、7 d蝇蛹的选择性与对照蝇蛹比较差异无统计学意义（P>0.05）；在表面喷洒球孢白僵菌孢子蝇蛹中羽化出的家蝇雌虫平均产卵前期为（6.00±0.94） d，对照组为（6.07±0.78） d，差异无统计学意义（P=0.200）；表面喷洒球孢白僵菌孢子蝇蛹中羽化出的家蝇和对照组家蝇的单雌产卵量分别为（152.20±14.37）和（633.17±17.08）枚，卵孵化率分别为（20.64±1.74）%和（88.63±2.05）%，雌蝇寿命分别为（4.93±1.74）和（28.93±2.70） d。家蝇蛹表面喷洒球孢白僵菌孢子可使其单雌产卵量和卵孵化率均下降为对照组的1/4，雌蝇寿命下降为对照组的1/6，生殖潜力下降为对照组的1%。结论在蝇蛹孳生地周围释放丽蝇蛹集金小蜂可控制80%以上的蝇蛹，同时在孳生地表面喷洒球孢白僵菌孢子，可使未被寄生蝇蛹中羽化出家蝇的生殖潜力下降近99%，从而达到双重控制家蝇的目的。

关键词: 丽蝇蛹集金小蜂, 球孢白僵菌, 家蝇, 综合生物防治

Abstract: Objective To provide scientific evidence for integrated control of Musca domestica, study the synergism of Nasonia vitripennis and Beauveria bassiana. Methods The "Y" olfactometer and host-selection tests were used to research the selectivity of female N. vitripennis to M. domestica pupae exposed to B. bassiana spores. Ecological simulation test was used to investigate the effects of B. bassiana on the reproductive potential and life-span of emerged M. domestica adults after being exposed to the spores at pupae stage. Difference of measurement data was compared with single factor analysis of variance using SPSS package. Results There was preference by female N. vitripennis to B. bassiana spores-treated M. domestica pupae and controls. The pre-oviposition period of M. domestica adults emerged from B. bassiana spores-treated pupae and controls were about(6.00±0.94) and(6.07±0.78) d, respectively, there were no statistical significant differences between them. The oviposition of female M. domestica adults emerged from B. bassiana spores-treated pupae and controls were(152.20) 14.37) and(633.17) 17.08) eggs, respectively. The hatchability of eggs ovipositted by flies treated with spores at pupae stage and controls were about(20.64±1.74)% and(88.63±2.05)%. The life span of female flies emerged from spore-exposed pupae and controls were about(4.93±1.74) and(28.93±2.70) d, respectively. So the female fecundity and egg hatchability of M. domestica treated with B. bassiana spores at pupae stage were reduced to 1/4 of controls, life span of female flies emerged from spore-exposed pupae dropped to 1/6 of controls, the reproductive potential of female flies emerged from spore-treated pupae dropped to 1% of control groups. Conclusion Beauveria bassiana spore treatment had no significant effects on the parasitic rate of female N. vitripennis to M. domestica pupae and the pre-oviposition period of emerged female flies. In the breeding grounds of M. domestica, releasing N. vitripennis could control 80% of the pupae. Spraying B. bassiana spores combined with releasing N. vitripennis could reduce the reproductive potential of M. domestica by 99%. The combined method is an effective means to the integrated biological control of M. domestica.

Key words: Nasonia vitripennis, Beauveria bassiana, Musca domestica, Integrated biological control

中图分类号:

R384.2
R376

黄振东, 万晴, 庄桂芬, 薛志静, 张瑞玲, 张忠. 丽蝇蛹集金小蜂和球孢白僵菌对家蝇的协同控制作用[J]. 中国媒介生物学及控制杂志, 2018, 29(4): 336-339.

HUANG Zhen-dong, WAN Qing, ZHUANG Gui-fen, XUE Zhi-jing, ZHANG Rui-ling, ZHANG Zhong. Synergestic control efficacy of Nasonia vitripennis and Beauveria bassiana against Musca domestica[J]. Chines Journal of Vector Biology and Control, 2018, 29(4): 336-339.

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丽蝇蛹集金小蜂和球孢白僵菌对家蝇的协同控制作用

Synergestic control efficacy of Nasonia vitripennis and Beauveria bassiana against Musca domestica

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