关键词: 球孢白僵菌, 丽蝇蛹集金小蜂, 安全性, 生物防治

Abstract: Objective To evaluate the safety of Beauveria bassiana CF08 strain on the fly pupal parasitoid, Nasonia vitripennis. Methods Insect-dipping test and spore-feeding method were used to evaluate the safety of B. bassiana CF08 strain on N. vitripennis larvae and adults. Results After spore-dipping or spore-feeding, the longevities of N. vitripennis female adults were (11.09±0.15)and (10.92±0.15)d, the control group was about (10.99±0.19)d, there was no significant difference among them (F=0.893, P=0.446). The longevity of spore-dipped, spore-fed and control male parasitoids were (5.83±0.09), (5.88±0.03), and (5.75±0.09)d, respectively. There was also no significant difference among the three groups (F=2.614, P=0.152). When the parasitized fly pupae dipped in the spore suspensions of B. bassiana CF08 strain, the parasitoid emergence rate, number of parasitoids emerged per fly pupa and sex ratio were more than 80%, 43 and 90%, respectively. The three indicators of spore-dripping groups and control group had no significant difference. Nasonia vitripennis emerged from spore-dripping fly pupae and control pupae had the same parasitic capacity to B. peregrine pupae. The parasitic rate, parasitoid emergence rate, number of parasitoids emerged from per fly pupa and sex ratio were more than 90%, 80%, 43 and 90%, respectively. There was also no significant difference among the spore-dripping groups and control group. Conclusion Beauveria bassiana CF08 strain had no negative effects on the development of immature stage and longevity of N. vitripennis adults. Exposure to B. bassiana spores at immature stage had no significant effects on the offspring quality of N. vitripennis. The parasitoid and entomogenous fungus could be used in combination for biological control of flies.

Key words: Beauveria bassiana, Nasonia vitripennis, Safety, Biological control