关键词: 蜚蠊, 分子鉴定, 细胞色素C氧化酶亚基Ⅰ

Abstract: Objective To provide basis for future molecular identification methods of cockroach species by analyzing gene sequences of cytochrome C oxidase subunitⅠ(COⅠ) among 15 cockroach species. Methods The mitochondrial COⅠ gene sequences of cockroaches were searched by NCBI and the sequence alignment was carried out by DNAStar software. An evolutionary divergence and a Neighbor-Joining tree were constructed using MEGA 6.0 software. Results The average interspecific genetic distances among 15 species of cockroaches in China was 0.166, and the genetic distances among species were significant. Phylogenetic tree analysis showed that COⅠ molecular markers were the same as traditional morphological classification, and the confidentiality more than 91. Conclusion The COⅠ gene can be effectively used to distinguish some species of domestic cockroaches. The value of 658 bp region of the mitochondrial COⅠ gene for identification of genus or subspecies is limited.

Key words: Cockroach, Molecular identification, Cytochrome C oxidase subunit Ⅰ