关键词: 收集螺卵恒温孵化法, 直接孵化法, 改良直接孵化法, 钉螺, 螺卵

Abstract: Objective To explore an easy and feasible method for hatching snail eggs in soil where snails existed. Methods On May 8^th, 2016, 20 soil samples were taken from Qingpu district where snails existed, and 3 units of soil which weighed 500 g respectively were used to carry on the experiment to hatch snail eggs in each piece of soil. The three methods included hatching snail eggs directly in the natural conditions, hatching eggs with improved conditions and hatching eggs with stable temperature. Kappa test was used to analyze the consistence of the results of the three methods. Kruskal-Wallis test was used to compare the hatching time, and χ² test was used to compare the three rates of hatching eggs. Results There was a statistical significance between methods with stable temperature and improved conditions in temperature (t=7.309, P=0.000). There were statistical significances in periods of hatching snail eggs among the three methods by Kruskal-Wallis test (χ²=269.340, P<0.001). There was no statistical significance in hatching rates with the three methods (χ²=5.880, P=0.053). Conclusion All the three methods can hatch snail eggs in the soil where snails existed, while the method hatching snail eggs directly under natural conditions canbe used as a complementary method since it needed simple experiment conditions.

Key words: Hatching with stable temperature, Hatching directly in natural conditions, Hatching with improved conditions, Oncomelania hupensis, Snail eggs