中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (5): 484-486.DOI: 10.11853/j.issn.1003.8280.2017.05.020

• 调查研究 • 上一篇    下一篇

黑龙江省同江口岸首次发现花鼠携带Bartonella washoensis的分子生物学证据

石金峰1, 耿聪2, 张坤1, 鞠文东2, 王力翔1, 王红霞1, 李振江1, 黄晓明1, 程成2   

  1. 1 同江出入境检验检疫局, 黑龙江 同江 156401;
    2 黑龙江国际旅行卫生保健中心, 哈尔滨 150001
  • 收稿日期:2017-06-23 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: 程成,Email:ate_0428@126.com
  • 作者简介:石金峰,男,助理兽医师,从事动物医学研究,Email:tongjiangciq@126.com
  • 基金资助:
    国家质量监督检验检疫总局科研基金(2016K115);黑龙江出入境检验检疫局科研基金(2016HK001,2015HK005)

Molecular evidence of Bartonella washoensis carried by Tamias sibiricus in Heilongjiang Tongjiang port

SHI Jin-feng1, GENG Cong2, ZHANG Kun1, JU Wen-dong2, WANG Li-xiang1, WANG Hong-xia1, LI Zhen-jiang1, HUANG Xiao-ming1, CHENG Cheng2   

  1. 1 Tongjiang Entry-Exit Inspection and Quarantine Bureau, Tongjiang 156401, Heilongjiang Province, China;
    2 Heilongjiang International Travel Healthcare Center
  • Received:2017-06-23 Online:2017-10-20 Published:2017-10-20
  • Supported by:
    Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China(AQSIQ)Scientific Research Subject(No. 2016K115)and Heilongjiang Entry-Exit Inspection and Quarantine Bureau Scientific Research Subject(No. 2016HK001, 2015HK005)

摘要: 目的 探索黑龙江省同江口岸鼠类中可能携带Bartonella washoensis的分子生物学证据。方法 2016年6月,在黑龙江省同江口岸林缘地中,采用夹捕法和笼捕法捕获啮齿动物,采集啮齿动物脾样本,利用巴尔通体rpoB基因序列保守区引物,用PCR法检测巴尔通体,获得的阳性样本通过基因测序进一步分析。结果 在捕获的113只鼠中共获得69份阳性样本,获得的序列与GenBank中的序列进行比对,在花鼠脾脏中获得的巴尔通体rpoB基因片段(编号:TJ2016-107)与同样是分离自花鼠的B. washoensis SR22-1株和SR25-1株同源性最高,分别为99%和98%。提示黑龙江省同江口岸花鼠中可能携带B. washoensis结论 首次在花鼠脾脏中扩增出与B. washoensis同源性较高的rpoB基因片段,这也是黑龙江省可能存在B. washoensis的直接证据。

关键词: 黑龙江省同江口岸, 花鼠, Bartonella washoensis

Abstract: Objective To explore the molecular biological evidence that rodents may carry Bartonella washoensis in Heilongjiang Tongjiang port. Methods Rodents were captured by night snaptrap and cagetrap method in June 2016. Spleen samples were aseptically collected to detect Bartonella by PCR of which primers were designed using the conserved region of the rpoB gene sequence of Bartonella spp. The positive samples obtained were further identified through gene sequencing. Results The results showed that 69 positive samples in all rodents, sequence alignment in GenBank showed that Bartonella rpoB gene fragment(No. TJ2016-107)obtained from Tamias sibiricus had high homology with SR22-1 and SR25-1 strain of B. washoensis, 99% and 98%. Conclusion It is the first time that the Bartonella rpoB gene positive fragments were amplified in the spleen of T. sibiricus, which is a high homology with B. washoensis. It is also the direct evidence that B. washoensis may exist in Heilongjiang.

Key words: Heilongjiang Tongjiang port, Tamias sibiricus, Bartonella washoensis

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