关键词: 家蝇, 乙酰胆碱酯酶, 残杀威, 残存活性

Abstract: Objective To measure the acetylcholinesterase (AChE) activity of Musca domestica in Hangzhou city, finding its correlation with the high resistance to propoxur. Methods Determiningpropoxur resistance level of M. domestica with topical application method, using colorimetric method to calculate the hydrolysis activity of susceptible strains and field strains, analyzing the residual activity of field strains. Results The LD₅₀ of field strains was higher than 400 μg, and the resistance coefficient was higher than 2 500. The AChE hydrolysis activity of susceptible strains and field strains were (0.291±0.114) and (0.360±0.104) μmol/(mg·min), respectively (t=2.692, P=0.009). The field strains in the proportion of 38.89% were insusceptible to propoxur. Conclusion The biochemical properties of AChE in Hangzhou city field strains were greatly changed as compared with the susceptible strains, the AChE hydrolysis activity increased and its susceptibility to propoxur declined, suggesting those may be the main causes of the high propoxurresistance of M. domestica in Hangzhou city.

Key words: Musca domestica, Acetylcholinesterase, Propoxur, Residual activity