嗜吞噬细胞无形体在不同组织细胞间抗原差异研究

doi:10.11853/j.issn.1003.8280.2017.03.015

中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (3): 255-257.DOI: 10.11853/j.issn.1003.8280.2017.03.015

嗜吞噬细胞无形体在不同组织细胞间抗原差异研究

高娃¹, 殷旭红¹, 郭生春¹, 乌兰图雅¹, 曹民治²

1 河套学院医学系, 内蒙古巴彦淖尔 015000;
2 巴彦淖尔市疾病预防控制中心, 内蒙古巴彦淖尔 015000

收稿日期:2016-12-29 出版日期:2017-06-20 发布日期:2017-06-20
作者简介:高娃,女,蒙古族,博士,讲师,主要从事蜱媒人兽共患疾病研究,Email:melody_gaowa@yahoo.com
基金资助:
巴彦淖尔市科技计划；河套学院引进人才科研启动基金

Analysis of antigenic diversity of Anaplasma phagocytophilum grown in different cells

Gaowa¹, YIN Xu-hong¹, GUO Sheng-chun¹, Wulantuya¹, CAO Min-zhi²

1 Department of Medicine, Hetao College, Bayannur 015000, Inner Mongolia Autonomous Region, China;
2 Byannur Center for Disease Control and Prevention

Received:2016-12-29 Online:2017-06-20 Published:2017-06-20
Supported by:
Supported by the Science and Technology Project of Bayannur, Inner Mongolia Autonomous Region and Scientific Research Foundation for Introduced Talents, Hetao College, Inner Mongolia Autonomous Region

摘要/Abstract

摘要： 目的调查嗜吞噬细胞无形体在不同组织细胞间产生抗原差异的原因。方法采用RT PCR法对嗜吞噬细胞无形体在不同组织细胞中的抗原蛋白表达差异进行分析，并利用真核无细胞蛋白表达系统合成重组蛋白，利用蛋白质印迹法进行测试。结果组织培养细胞不同，嗜吞噬细胞无形体菌体表面可产生不同的P44外膜蛋白。通过检测发现，3种rP44蛋白易与患者血清中抗体结合，可作为人粒细胞无形体病的诊断用抗原。结论不同组织细胞间存在抗原性差异的不同嗜吞噬细胞无形体。

关键词: 嗜吞噬细胞无形体, P44外膜蛋白, 无形体病

Abstract: Objective To analyze the reasons for antigen diversity of Anaplasma phagocytophilum grown in different cells. Methods The P44 transcripts were analyzed using nested RT-PCR, and the P44 proteins were produced as recombinant protein in vitro by insect cell-free protein synthesis system. Results We detected and characterized P44 transcript variants of A. phagocytophilum from THP-1 and HL60 cells. And our study demonstrated the presence of specific antibodies against the 3 type of rP44 proteins that prevailed in infected THP-1 and HL60 cells, probably being suitable as protein antigens for sero-diagnosis of human granulocytic anaplasmosis. Conclusion We demonstrated antigenic diversity of a variety of A. phagocytophilum grown in different cells.

Key words: Anaplasma phagocytophilum, P44 outer membrane protein, Anaplasmosis

中图分类号:

R376

高娃, 殷旭红, 郭生春, 乌兰图雅, 曹民治. 嗜吞噬细胞无形体在不同组织细胞间抗原差异研究[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 255-257.

Gaowa, YIN Xu-hong, GUO Sheng-chun, Wulantuya, CAO Min-zhi. Analysis of antigenic diversity of Anaplasma phagocytophilum grown in different cells[J]. Chines Journal of Vector Biology and Control, 2017, 28(3): 255-257.

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嗜吞噬细胞无形体在不同组织细胞间抗原差异研究

Analysis of antigenic diversity of Anaplasma phagocytophilum grown in different cells

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